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Dr3
Stranger


Registered: 05/23/19
Posts: 96
Last seen: 11 months, 16 days
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Help understanding my AGAR contam
#26887908 - 08/19/20 07:57 AM (3 years, 5 months ago) |
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Greetings folks, I've generally been succesful with agar, but recently I0ve been having wierd contamination all over my plates. Control plates (the ones I didn't transfer to) seem to be fine.
I try to follow proper sterile technique, scalpel was wimped with 70 ISo and flamed, etc. What I'd like to know is whether the contam might have transferred from the original plate. Perhaps you can shed some light on this.
Regardless, I'll prolly should try to transfer from a different plate. Maybe I waited too long and there are contams on top of the myc which are invisible. Though the spread on the receiving plates suggests to me that contam could be from a external vector.
These are the pics:

original plate:

thanks for your help as always
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dirtybirdx
getting better

Registered: 07/28/20
Posts: 250
Last seen: 2 years, 3 months
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Re: Help understanding my AGAR contam [Re: Dr3] 1
#26887969 - 08/19/20 08:45 AM (3 years, 5 months ago) |
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It’s hard to say.
But the point of agar is to assure your cultures and give you some extra confidence.
So when we aren’t sure, we transfer. We try all the time to target the one vector that seems to be giving us contam issues, but it’s often a mystery.
You have to remember the ever present battle, like good vs evil — mycelium vs contam.
There will always be contams, there will always be some vector(s) that you have to deal with.
Many of us deal with them not by perfectly escaping every contaminate vector and killing sterilizing 100%, but instead by starting with healthy mycelium.
Healthy mycelium will fight and eat some contaminates and it has a better chance of maturing to fruits. Can weak mycelium make it? Of course, but the risk for contam is higher within every vector.
Best thing to do is make some more plates, transfer the good looking stuff, leave behind the questionable stuff.
It’s ok to throw away questionable culture, even if it was fine. (IMO )
-------------------- Need help?

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Roger Clemency
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Registered: 03/23/20
Posts: 2,005
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Re: Help understanding my AGAR contam [Re: dirtybirdx] 1
#26888040 - 08/19/20 09:30 AM (3 years, 5 months ago) |
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I’m not sure what I’m looking at in those plates. Did you transfer just one wedge into them? The yellowish looking spot near the center, and all those others are satellite colonies?
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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dirtybirdx
getting better

Registered: 07/28/20
Posts: 250
Last seen: 2 years, 3 months
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Quote:
Roger Clemency said: I’m not sure what I’m looking at in those plates. Did you transfer just one wedge into them? The yellowish looking spot near the center, and all those others are satellite colonies?
They look like germ plates, but he said they were transfers 🤷♂️ And you can see the agar wedge.
Some funky plates....
-------------------- Need help?

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Dr3
Stranger


Registered: 05/23/19
Posts: 96
Last seen: 11 months, 16 days
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Re: Help understanding my AGAR contam [Re: dirtybirdx]
#26891167 - 08/21/20 07:06 AM (3 years, 5 months ago) |
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thank you all for your answers. Yes, they are small wedges, I figure the smallest they are the better. For my next attempt I'll transfer from a dif. plate and I'll see what happens.
A huge mistake I make is probably not using sleeves and exposing my forearms and such, so I'll also start using long sleeves. That'll help prolly.
Cheers
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