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Invisiblemuse_sick
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agar question
    #2688762 - 05/16/04 10:57 PM (12 years, 11 months ago)

can an already fully colonized plate of agar become contaminated?


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Re: agar question [Re: muse_sick]
    #2688983 - 05/16/04 11:37 PM (12 years, 11 months ago)

yes.


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Invisiblemuse_sick
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Re: agar question [Re: ]
    #2689517 - 05/17/04 01:28 AM (12 years, 11 months ago)

what does it look like?


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InvisibleATWAR
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Re: agar question [Re: muse_sick]
    #2689539 - 05/17/04 01:32 AM (12 years, 11 months ago)

Depends on what type of contamination you have. Bacteria contaminated mycelium would be very difficult to see, if not impossible...

Try looking here.


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Invisiblemuse_sick
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Re: agar question [Re: ATWAR]
    #2689581 - 05/17/04 01:42 AM (12 years, 11 months ago)

no it doesn't look like any of that
all of the contams shown on the faq on agar are very blatant
ie they are brown or green
this is more like white spots
it could almost be rhizomorphic growth
but it is more dense than the surrounding growth
i have seen contams on casings, in jars, etc.
and it doesn't appear to be like any of them
but this is my first time with agar


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Re: agar question [Re: muse_sick]
    #2689615 - 05/17/04 01:49 AM (12 years, 11 months ago)

Does it look like mycelium? Does it look like hyphal aggregates? You say it could be rhizomorphic growth, does it look like twisted strings? I have had white bacterial contamination on agar before, almost looks like drops of milk, not mycelium.

Sounds like the beginning of pins on agar to me... Perhaps you could describe it better, or post a picture. This belongs in the contamination forum anyway...


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Invisiblemuse_sick
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Re: agar question [Re: ATWAR]
    #2689682 - 05/17/04 02:12 AM (12 years, 11 months ago)

sorry i can't post a pic right now
i guess it doesn't really matter
i can just do a transfer anyway
but it does sound like hyphea
now that you mention it
i prob just couldn't recognize b/c i've never worked w agar before

the contam forum never gets browsed, ime
i would've had to wait till tmrw to get these answers
and another three days to get the dialogue we just had here


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InvisibleATWAR
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Re: agar question [Re: muse_sick]
    #2689707 - 05/17/04 02:22 AM (12 years, 11 months ago)

It most likely is the beginning of knots, which just goes to show you have an isolate that is very willing to fruit. I would select the best growth and transfer it to another plate. Keep an eye on the other one.

If it looks like little cotton balls, then it is hyphal aggregates (or knots).


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