|
junior c
junior c


Registered: 04/08/20
Posts: 42
|
breeding shiitake
#26886677 - 08/18/20 12:41 PM (3 years, 5 months ago) |
|
|
Greetings shiitake heads. May 13 I inoculated a few jars with a mixed spore solution (via S75). Substrate in jars is a sterilized mix of hwfp, coir, gypsum and maybe a bit of wheat bran. Last week I slapped them around a bit to see how consolidated the sub was and if it would be possible to pop out of the jar intact. Was starting to notice browning and was/am considering putting them into fruiting conditions. Anyhow, this week I notice what at first I thought was pop-corning, but now think is a fruit (see pic). The current plan is to: 1)clone this potential fruit 2)move to grain 3)move to sub bags 4)monitor colonization and pray for a nice flush
My guess is banging the jars triggered the pin, but could be wrong. Jars have not gone below 68F, so it wasn't a cold shock. Any advice on this is appreciated. I recall reading RR say successfully breeding a good producing shiitake was a long shot. What say you? thanks shroom board.

-------------------- "Existence has overpowered books. Today, I slew a Mushroom" - Emily D
|
NPoB
Curious

Registered: 01/24/16
Posts: 93
Loc: Mendocino County
Last seen: 13 days, 13 hours
|
Re: breeding shiitake [Re: junior c]
#26886864 - 08/18/20 02:14 PM (3 years, 5 months ago) |
|
|
My best performing shiitake strain came into my hands through blind luck starting with a spore print so it *can* happen even if unlikely.
I'd suggest NOT trying to do anything with those jars. Starting again using grain in the jars and transferring to sawdust media in a bag well prior to the brown appearing will give you better results. Also, I'd suggest starting out on agar before taking it to grain. There are good reasons that is such a common approach.
|
Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 24 days, 17 hours
|
Re: breeding shiitake [Re: NPoB]
#26887020 - 08/18/20 04:01 PM (3 years, 5 months ago) |
|
|
I assume you did mean clone that fruit to agar first to make sure it's clean before grain, as mentioned.
In my experience the slap never did anything, it was always cold that got mine to pin, but you say it didn't get cold so who knows?
Interested to see how this goes for ya
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
|
junior c
junior c


Registered: 04/08/20
Posts: 42
|
Re: breeding shiitake [Re: Forrester]
#26887055 - 08/18/20 04:29 PM (3 years, 5 months ago) |
|
|
Thanks for the response. Happy to hear of the spore print success. It's encouraging. Way back in May I just had a little extra sub and thought I'd throw some spores in, see what happens. A nice zero effort experiment.
Sorry. I wasn't very clear. When I said clone, I did mean removing clean/inside tissue from the blob (fruit?) in the middle jar and cleaning it/monitoring it's growth on agar prior to transferring the mycelium to grain.
NPoB, are you suggesting: 1)add a mixed shiitake spore solution to grain 2)later inoculate sawdust bags with this grain 3)Grow out & attempt to fruit these sawdust blocks 4)Start new strain by cloning any successful mushrooms?
I'm very new to breeding mush. My assumption was these jars are currently a collection of competing dikaryon populations and the goal was to isolate one of these populations. Preferably obtaining the one dikaryon pop. that fruits nice mush, grows fast etc.
What are the best ways to find this? I'm guessing spores to agar and observing & selecting/combining successful hyphae has the highest batting average. But could you also potentially obtain a decent dikaryon isolate by carving out mycelial tissue (fruit body)?
Forrester. What kind of shiitake do you grow? It's funny you say that. I've noticed S75 doesn't need a cold shock.
-------------------- "Existence has overpowered books. Today, I slew a Mushroom" - Emily D
|
Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 24 days, 17 hours
|
Re: breeding shiitake [Re: junior c]
#26887167 - 08/18/20 05:37 PM (3 years, 5 months ago) |
|
|
Ah man it's been a few years, I can't even remember the names of the strains I had. 75 was probably my least favorite. I think it was 3750 I liked most, but I can't remember. All my cultures are gone, I will have to start over when I get back into it.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
|
NPoB
Curious

Registered: 01/24/16
Posts: 93
Loc: Mendocino County
Last seen: 13 days, 13 hours
|
Re: breeding shiitake [Re: junior c]
#26895048 - 08/23/20 09:22 AM (3 years, 5 months ago) |
|
|
What I'd suggest: Start out with agar on plates. Select the best looking sectors you see (this is what you are describing wanting) and transfer to agar on another plate. Move the growth from that to another plate to be sure you have nice uniform growth (if not, try transferring another nice looking sector to another plate). Your goal here should be getting a strain out of what grows from your spores and hoping it performs well when you test it through fruiting. (Make a duplicate plate, let it get a nice start, label it and move it into a refrigerator while you test its performance. As soon as you get a good strain be sure to make up some master slants. Do this for every sector you test.) Move the growth from that onto grain. Use the grain to either make more grain or else inoculate sawdust in bags. Fruit the sawdust in bags and keep good records on your results so that you can compare your strains.
Even grain in jars will form shiitakes if neglected long enough but for me the shiitakes seem to prefer forming at or near the bottom of the jar where they can't be harvested. John Holliday told me unused grain can be permitted to fruit by opening the jars and laying them on their side but that has never worked for me.
Roger Rabbit put out a nice tutorial called Let's Grow Mushrooms on DVD. Older versions are online. This is what got me started (and hooked on) growing mushrooms. It is well worth buying the DVD.
|
NPoB
Curious

Registered: 01/24/16
Posts: 93
Loc: Mendocino County
Last seen: 13 days, 13 hours
|
Re: breeding shiitake [Re: Forrester]
#26895052 - 08/23/20 09:25 AM (3 years, 5 months ago) |
|
|
75 was the single most overhyped shiitake I've grown.
|
|