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InvisibleTheDoobskerS
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Registered: 05/22/20
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Wadup with this Nepal Chitwan shoebox
    #26886548 - 08/18/20 11:20 AM (3 years, 5 months ago)

I had a previous post about my cleaning of these spores on agar.
https://www.shroomery.org/forums/showflat.php/Number/26830642

I was informed that the plates were probably trash and had meshed in mold. I decided to go ahead and inoculate grain and spawn to a shoebox anyway for the experience (I've only done pf tek in the past). I just made sure to keep the jars and shoebox far away from my other grows.

Pulled my last transfer from this plate:


Inoculated oats from this plate (photos @ 11 & 33 days of growth):


Let the oats colonize for about 2 weeks (man, did they smell delish at spawn):


______________________________________________________________________
Spawned the 2 pints of colonized oats to coco coir using SFF tek:
https://www.shroomery.org/forums/showflat.php/Number/26009662

@ 6 days after spawn


today @ 8 days after spawn


______________________________________________________________________
I was expecting to get a moldy mess that needed to be thrown away asap. I have a few questions now that I've let this play out a bit:

1) How could it have mold that appears to grow at the exact same rate as the myc? Or did I successfully isolate the myc from mold with that last transfer?

2) I see a good amount of knots on the surface, but the bottom of the tub is going nuts with knots and pins compared to the rest. My coir was too dry when I spawned, and I didn't do a good enough job of tamping it all down. So I'm not surprised to see lots of side pins/knots, but why so much on the bottom?

3) If I end up getting a healthy flush, any good tricks on harvesting bottom shrooms? Or am I gonna need to just pick up the sub and flip it over while floating?

4) Why is it going so fast? Pins @ 8 days seems way too soon. It's been hovering between 72-80F in my house

5) The surface looks a bit dry to me in some areas, should I mist it or wait it out?


Anyways thanks in advance for any input


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OfflineOne of Us
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Registered: 03/12/12
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Re: Wadup with this Nepal Chitwan shoebox [Re: TheDoobsker]
    #26886598 - 08/18/20 11:58 AM (3 years, 5 months ago)

I can answer 4 and 5

4. It is probably fast because the mycelium senses a contam and is trying to hurry up and fruit/produce spores while it still can, before the contamination takes over.

5. It definitely looks dry. Give it a good misting. No need to wait.


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InvisibleGoatrider
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Registered: 04/08/20
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Re: Wadup with this Nepal Chitwan shoebox [Re: TheDoobsker]
    #26886618 - 08/18/20 12:13 PM (3 years, 5 months ago)

Let`s see if i can give some good answers :wink:

1. Hard to say, but the second plate, where you inoculated from, is nearly grown out to the border. High contam risk waiting there.

2. You see it on brf cakes. Lots are pinning at the bottom, as there is more moisture.
Best choice for me is the ShaperDreaming tek, with the sub under field capacity,
and a heavy misted layer on top, to provide best surface conditions.

3. If i don`t use a liner, i always get sides and bottoms due to heavy temperature swings in my house. After harvesting the top,
i take a sheet of aluminium foil and lay on surface.
I take a big book that fits in the box then and lay it on top, flip over and harvest sides and bottom.

4. They may want to get out of a contam situation i guess. 8 days is very fast.

5. Really hard for me to tell from pictures, but i always want huge amounts of tiny droplets until knots are forming.

Perhaps this helps a bit.

Edit: One of us a bit faster :thumbup:


          :cookiemonster:


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InvisibleTheDoobskerS
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Registered: 05/22/20
Posts: 799
Re: Wadup with this Nepal Chitwan shoebox [Re: Goatrider]
    #26886712 - 08/18/20 12:53 PM (3 years, 5 months ago)

Cool thanks for the replies.

So I guess the 8 days factor kinda answers the question of having contams or not. But, I'm wondering what kinda contam this would be? With mold, I only have experience with trich, and wouldn't that be growing out of control and taking over? Especially since the trich would have come from the plate and not a later stage. Whatever it is, they seemed to have grown really cooperatively.

Nothing seems to have slowed its growth at all during any stages. But my inoculation plate sure didn't look like like nice happy myc.

I think I can expect some fruits, but I've been really scratching my head about what's actually going on with that plate that I used.


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