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OfflineSumpfkroete
Myceliotropic
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Registered: 08/18/20
Posts: 8
Last seen: 1 year, 1 month
My second grow and again mold in the Spawn Bag? Help please
    #26886125 - 08/18/20 06:13 AM (3 years, 5 months ago)

Hey dear Shroom-friends!

First of all, I want to thank the universe for forums like this one, where beginners like me can come in contact with professionals like you.

As the Subject says, I made my second try to grow shrooms by my own in a monotube.

Unfortunately (like the last try 2 years ago) I fucked the inoculation process up and spotted (I guess) green mold in my Spawn bag...

Before I started to inoculate I informed myself with hundreds of Videos, methods, how to inoculate tutorials and whatever. Nevertheless somehow a not wanted spore made it's way into my Spawn bag and I really can't tell why...

Here is my Setup:

- I bought the Spawnbag from a Website here in the netherlands where I live.
It looked great and had no issues.

- To inoculate I used a Spore Syringe With Golden Teacher spores
  (and used the whole Syringe to inoculate faster) which I bought in
  a Shop in my City.

- I used 4 different Sterilization liquids (70% Isopropanol, 2 hand sanatizers, and a surface disinfectent.

- Also I had a hairnet, mouthmask and new sterile gloves.

- Before I started to Inoculate (In my little Bathroom I cleaned everything more than enough and disinfected everything after. Also the Spawnbag and the Syringe.

- I didn't touched the sterile syringe top and flame sterilized for 20 seconds and whiped the top with Isopropanol.

- I closed all windows and even had a small container in which I tried to keep everthing in so the Airflow is minimized (It's not a still-air container)

I really did my best to avoid any potential contamination and it took nearly 45 minutes to disinfect everything and get the stuff I don't need out of the way.

I was so sterile that I was a 100% sure It should work but as it seems I was not...

5 Days now after inoculating, the mycelium grows nicely and hyphae strings are going to colonize the grain. Besides the nice Mycelium there are green/blue looking spots in the mycelium which I can tell is absolutely no bruising or something (picture is included). Somehow the Spots are only where the mycelium is.

Could it be that the Syringe was contaminated?

Because the mold only appears where the mycelium appears. There are no spots where only the mold is showing.


Can you guys give me some tips to be even more sterile? This is the second time I fucked up 50€ for the syringe and spawnbag...

I really don't know what I do different from all the Videos I watched.

Thanks in advance and have a great day Guys!










--------------------
See them as tool, use them with wisdom :mushroom2:



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Offlinefumble1130
Shameless Hippie
Male
Registered: 07/26/20
Posts: 28
Loc: New Mexico, USA
Last seen: 9 months, 23 days
Re: My second grow and again mold in the Spawn Bag? Help please [Re: Sumpfkroete]
    #26886149 - 08/18/20 06:40 AM (3 years, 5 months ago)

Pics aren't showing for me, but I wouldn't trust grain bags bought online. I've had close to 50% failure rate with them.


Edited by fumble1130 (08/18/20 06:41 AM)


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InvisibleLadysKnight
Hello Ladies
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Registered: 10/09/15
Posts: 1,670
Re: My second grow and again mold in the Spawn Bag? Help please [Re: fumble1130]
    #26886187 - 08/18/20 07:17 AM (3 years, 5 months ago)

All prints and syringes are contaminated.
You cannot inoculate in open air, bathrooms are the worst.
You cannot wipe a sterile needle with alcohol.


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OfflineEntheoGod
Entheo
Male User Gallery


Registered: 03/06/15
Posts: 648
Last seen: 7 months, 27 days
Re: My second grow and again mold in the Spawn Bag? Help please [Re: LadysKnight]
    #26886206 - 08/18/20 07:28 AM (3 years, 5 months ago)

Look in my signature.

Learn agar my friend. It’ll be a much better experience for you. All sources of spores have potential for contamination and agar allows you to be sure you have clean inoculant. 👍

Also, don’t buy sterile supplies online. Swabs and scalpels are different than attempting to buy clean grain online.


Edited by EntheoGod (08/18/20 07:30 AM)


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OfflineSumpfkroete
Myceliotropic
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Registered: 08/18/20
Posts: 8
Last seen: 1 year, 1 month
Re: My second grow and again mold in the Spawn Bag? Help please [Re: EntheoGod]
    #26886223 - 08/18/20 07:41 AM (3 years, 5 months ago)

Thanks for your Response mates.

So it is not possible to do this without agar?

I don't have a pressure cooker and It would be much money I need to spend then...

Is it maybe better to build a still air box in which I can inoculate?

I have never informed myself about agar :confused:

Isn't it that I need even more patience with agar?

Thanks in advance :smile:

The pictures can be opened by right clicking them and click on "Show in new tab"


--------------------
See them as tool, use them with wisdom :mushroom2:



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Offlinefumble1130
Shameless Hippie
Male
Registered: 07/26/20
Posts: 28
Loc: New Mexico, USA
Last seen: 9 months, 23 days
Re: My second grow and again mold in the Spawn Bag? Help please [Re: Sumpfkroete]
    #26886235 - 08/18/20 07:50 AM (3 years, 5 months ago)

Have you tried PF Tek? From what I understand, it's pretty much the way to go if you don't have a pressure cooker.


Edited by fumble1130 (08/18/20 07:51 AM)


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