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Unknonw
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Registered: 09/19/17
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Are spore syringes a crap shoot?
#26879133 - 08/13/20 07:19 PM (3 years, 6 months ago) |
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I knocked up 10 WBS jars two weeks ago, and three of them are almost at the 30% colonization point. While the other 7 jars are all lifeless, except for one that has a needles worth growing. Am I doing something wrong here?
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Mad Hatter
10/6



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Re: Are spore syringes a crap shoot? [Re: Unknonw]
#26879139 - 08/13/20 07:21 PM (3 years, 6 months ago) |
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Spores can take a while to germinate and spores from the same syringe will germinate at different rates. Just because they havent germinated yet doesnt mean they wont.
The reason they're consider crap shoots is that they arnt clean and you get random genetics.
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Tight Lunchbox
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Re: Are spore syringes a crap shoot? [Re: Unknonw]
#26879156 - 08/13/20 07:37 PM (3 years, 6 months ago) |
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If you have the means to sterilize grains I suggest buying a SAB and some agar supplies. It's stupid easy and it'll allow you to grow a pile of mushrooms a bunch of different ways.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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mushhead
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Spore syringes can carry bacteria and even mold sometimes. MS projects are also slow to germinate as well, which is why you should look into agar and build yourself an SAB; on the other hand you can get a flow hood which is imo the best way to go once you get better at agar. You should do one of two things imo with a MS Syringe. 1: Make up some BRF jars and allow to fruit to obtain spore which you then start on agar. 2: Drop small droplets of spores from the syringe directly onto an agar dish to germinate.
Number one is my go to as I like to see how the fungus will fruit and grow. I then use an SAB to collect spores and start a larger project with them . Number two is a very common practice amongst us here and in many cases succeeds enough to yield nice plates after a few transfers. Number two can also leave you with... lots of bacteria sometimes... too many times in my case which is the reason for number one.
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Unknonw
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Re: Are spore syringes a crap shoot? [Re: mushhead]
#26884978 - 08/17/20 02:03 PM (3 years, 5 months ago) |
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I ordered another syringe, as I do not have faith in 6 out of 10 of the jars. Reinject the WBS jars, or start over with fresh ones?
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Rhizomorph
Psychedelic Researcher



Registered: 04/24/20
Posts: 786
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Re: Are spore syringes a crap shoot? [Re: mushhead]
#26884996 - 08/17/20 02:13 PM (3 years, 5 months ago) |
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Why not MS syringe -> BRF -> cloning on agar? This seems like the best way to start out with better genetics on agar since you can pick the genetics from the best looking fruit.
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Unknonw
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Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
#26885015 - 08/17/20 02:21 PM (3 years, 5 months ago) |
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Wouldn't I have to first PF Tek and clone? Or no? Couldn't I just power through the wbs and clone.
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A.k.a
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Re: Are spore syringes a crap shoot? [Re: Unknonw]
#26885126 - 08/17/20 03:23 PM (3 years, 5 months ago) |
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If you have a clean syringe yes, I got four from one of the biggest vendors when I started and they were all totally clean. Later I found out itβs because they use centrifuges to separate spores which also removes contams.
You could use brf as agar, drip a tiny bit from the syringe and wait for clean myc to grow and then take that and use it to inoculate grains.
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LAGM2020     
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EntheoGod
Entheo



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Re: Are spore syringes a crap shoot? [Re: Unknonw]
#26885167 - 08/17/20 03:46 PM (3 years, 5 months ago) |
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I want to respond to this with a simple.. yes.
Spore syringes are always crapshoots unlessssssss made in perfectly sterile and clean conditions.
I may sound angry π lol but thatβs because I have a grudge against ms syringes because of so many bad experiences when put to grain. You can get great results out of spore syringes but I recommend using agar to clean the syringe up. ππ
Edited by EntheoGod (08/17/20 03:47 PM)
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Rhizomorph
Psychedelic Researcher



Registered: 04/24/20
Posts: 786
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Re: Are spore syringes a crap shoot? [Re: Unknonw]
#26885254 - 08/17/20 04:23 PM (3 years, 5 months ago) |
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I was mainly responding to Mushhead's "second option".
You can continue with the wbs but I highly recommend at the end of your grow cloning to agar and then avoiding syringes where possible once some decent shrooms come up. You can even clone mushrooms that grow from contaminated substrate & then clean it up on agar.
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jcm4620
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Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
#26885424 - 08/17/20 05:30 PM (3 years, 5 months ago) |
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i lnow i have posted this pic a fuck ton but its a great example of why to use agar and it shows that even syringes from top tier vendors are not always clean. spores themselves are dirty to some extent unless the fruits they came from were grown in an absolutely sterile environment. for the most part all spores will contain what ever contams are in the air of the environment they were grown in but this syringe came from what is if u ask me the very best vendor there is
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shroomrookie
truth seeker



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Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
#26885682 - 08/17/20 08:22 PM (3 years, 5 months ago) |
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Quote:
Rhizomorph said: Why not MS syringe -> BRF -> cloning on agar? This seems like the best way to start out with better genetics on agar since you can pick the genetics from the best looking fruit.
This is exactly what I do. I collect my own spores and have forever. I've been doing brf PF tek so long. It so easy. Then clone and done. Its easy to get some relatively clean prints to work with. I only get 1/10 contam so this is easy and convenient for me. I've skipped the agar and went straight to LC before. Once you get your clean technique down its not hard.
If I buy a new syringe to try something new I do this. I've gotten scores of syringes from *(&&$^$% and never a one contam with pf brf tek. My luck I guess. Im not talking about bacteria. Bacteria doesn't stop you from cloning or printing your shrooms.
..and to tell the truth I don't get much or any bacteria either.
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Edited by shroomrookie (08/17/20 08:28 PM)
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Rhizomorph
Psychedelic Researcher



Registered: 04/24/20
Posts: 786
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Re: Are spore syringes a crap shoot? [Re: jcm4620]
#26885727 - 08/17/20 08:57 PM (3 years, 5 months ago) |
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Quote:
jcm4620 said: spores themselves are dirty to some extent unless the fruits they came from were grown in an absolutely sterile environment.
I.e pins growing in vitro on agar. If somebody really wanted a sterile print/syringe they should do this.
But then why not just do agar transfers haha. I suppose if one were still using BRF cakes but I don't have the patience to wait for them to colonize 
For the sake of mailing prints/syringes though...
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jcm4620
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Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
#26885732 - 08/17/20 08:59 PM (3 years, 5 months ago) |
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agar is the way to go always no matter what ππ
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Rhizomorph
Psychedelic Researcher



Registered: 04/24/20
Posts: 786
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Re: Are spore syringes a crap shoot? [Re: shroomrookie]
#26885735 - 08/17/20 09:00 PM (3 years, 5 months ago) |
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1/10 contam is a reasonably high rate of contamination if you ask some people on here. Not that my technique is much better, but both of us can definitely reduce that with better technique unless you're living in contamination central.
It does add up over time...
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Rhizomorph
Psychedelic Researcher



Registered: 04/24/20
Posts: 786
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Re: Are spore syringes a crap shoot? [Re: jcm4620]
#26885739 - 08/17/20 09:01 PM (3 years, 5 months ago) |
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All hail the lord of agar
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jcm4620
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Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
#26885745 - 08/17/20 09:07 PM (3 years, 5 months ago) |
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really u can take a steaming shit on your work table right next to your sab and as long as your methods and practices are good its not even an issue my house is trich fucking central i swear but i have a very very low contam rate so i dont think environment matters as much as good sab/fh practices matter know what im sayin
but im not sayin u should shit on ur table lolπ©π©ππ
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Celestialexplorer1



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Re: Are spore syringes a crap shoot? [Re: jcm4620]
#26885759 - 08/17/20 09:13 PM (3 years, 5 months ago) |
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I donβt know if it was the chocolates I ate earlier or the visual I got from reading that but it cracked me up.
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 To spend just one moment in eternity
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jcm4620
Stranger


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lol glad ya get my sick sense of humorππ
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shroomrookie
truth seeker



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Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
#26885764 - 08/17/20 09:16 PM (3 years, 5 months ago) |
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Quote:
Rhizomorph said: 1/10 contam is a reasonably high rate of contamination if you ask some people on here. Not that my technique is much better, but both of us can definitely reduce that with better technique unless you're living in contamination central.
It does add up over time... 
Maybe but Im only guessing at the 1/10. I do alot of syringes. I made one contaminated syringe back in feb. If I use prints only a few days old they colonize super fast. Agar is the way to go for bulk grows where the loss due to contamination is high. I wouldn't want to chance bulk with MS.
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