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OfflinePsychedelicjunkie
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Registered: 07/05/20
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Soap or 70% iso for SAB
    #26881961 - 08/15/20 04:27 PM (3 years, 5 months ago)

Hello i have read that cleaning my SAB with soap water and letting Some water stay at the bottom, is the best way to prepare my SAB. Or is it Wrong? Maybe 70% iso is Better for it?


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OfflineBiscuits
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Re: Soap or 70% iso for SAB [Re: Psychedelicjunkie]
    #26882428 - 08/15/20 09:47 PM (3 years, 5 months ago)

Clean it with whatever you want -- soap, iso, whatever. As long as it cleans it.

Then usually a small spraying of soapy water on the walls and floor in order to catch airborne contaminants -- they will stick to the soapy water more than a dry plastic surface. It sounds like you're going to leave a puddle of dirty water in the bottom of the tub... I wouldn't do that.


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Offlinemeowjinx
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Re: Soap or 70% iso for SAB [Re: Biscuits]
    #26882499 - 08/15/20 11:17 PM (3 years, 5 months ago)

Most people who wet the bottom do so by using a towel. I use a bath towel, other types may work too

You'd place the SAB over the towel and spray it with soap water. This would usually involve not using the lid and putting something heavy on top of the SAB to ensure that it presses against the towel and creates a vacuum


Edited by meowjinx (08/16/20 09:46 AM)


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Offlinerocklobster
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Re: Soap or 70% iso for SAB [Re: meowjinx]
    #26882543 - 08/16/20 12:04 AM (3 years, 5 months ago)

I've been having good results using this tek from Bod. I clean the SAB and the table surface with ISO. Let it evaporate then use a soapy water mixture to prepare it. Spray the sides, top and floor of the SAB. I try to go lighter on the front so I can see what I'm doing a little better.


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Invisiblemaxmush
Always learning...
Registered: 06/13/20
Posts: 440
Re: Soap or 70% iso for SAB [Re: rocklobster]
    #26882550 - 08/16/20 12:13 AM (3 years, 5 months ago)

I have been cleaning with iso with final a mist (try a small travel hairspray mister) upwards through the arm holes with great success. I then leave it for several minutes before using (about 10min).

I do this for 2 reasons:

1. to "catch" any airborne particulates
2. to create a moist bottom layer for particulates to fall on (which can then be neutralized by he iso)

I will sometimes put an iso misted paper towel at the bottom but not always.


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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OfflineYour Dudeness
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Re: Soap or 70% iso for SAB [Re: maxmush]
    #26882586 - 08/16/20 12:43 AM (3 years, 5 months ago)

Water is full of contams its best to use isopropy you don't see the nurse wiping your arm down with soapy water do ya?  Naw she uses that alcohol son!


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RR sent me


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OfflineRapjack
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Re: Soap or 70% iso for SAB [Re: Your Dudeness]
    #26882765 - 08/16/20 04:36 AM (3 years, 5 months ago)

Quote:

Your Dudeness said:
Water is full of contams its best to use isopropy you don't see the nurse wiping your arm down with soapy water do ya?  Naw she uses that alcohol son!




That's the point!
Misting the air captures most of the nasties floating around and traps them. Sterile technique and sanitizing everything takes care of the rest. Using iso instead of soapy water which evaporates very quickly would be counterproductive. I do wipe the inside of my SAB with iso before using it though.

I just follow Bod's TEK, it always worked wonderfully once I got the technique down.


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Edited by Rapjack (08/16/20 04:36 AM)


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OfflinePsychedelicjunkie
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Re: Soap or 70% iso for SAB [Re: Rapjack]
    #26882915 - 08/16/20 07:46 AM (3 years, 5 months ago)

Okay thanks for the advice guys  :smile:


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OfflineEl Chupacabra
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Re: Soap or 70% iso for SAB [Re: Psychedelicjunkie]
    #26882946 - 08/16/20 08:11 AM (3 years, 5 months ago)

OP, just saying this since nobody else did, iso alcohol is flammable (errbody knows that tho). Some have accidentally made their SAB into arm incinerators by trapping all the alcohol fumes add flame and... BOOM!

Just wanted to share that word of caution with a newbie, don't be the next fool.

EDIT: I should have said that you can absolutely use it, and you should. Just use common sense and let the alcohol evaporate before enclosing it with your work inside and potentially your arms.


Edited by El Chupacabra (08/16/20 08:18 AM)


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OfflineItsaMeShroomio
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Re: Soap or 70% iso for SAB [Re: El Chupacabra]
    #26882994 - 08/16/20 08:38 AM (3 years, 5 months ago)

I used to use ISO, but I noticed donor plates tend to freak out after being exposed to iso-heavy air. The edges almost always start growing up into the air and turn blue after being in iso air.


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OfflinePsychedelicjunkie
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Re: Soap or 70% iso for SAB [Re: ItsaMeShroomio]
    #26883161 - 08/16/20 10:06 AM (3 years, 5 months ago)

Thanks for the warning


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InvisiblePastywhyteMDiscord
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Re: Soap or 70% iso for SAB [Re: Psychedelicjunkie]
    #26883168 - 08/16/20 10:11 AM (3 years, 5 months ago)

I’ve used both. Both work fine.


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Offlinerocklobster
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Re: Soap or 70% iso for SAB [Re: El Chupacabra]
    #26883257 - 08/16/20 10:58 AM (3 years, 5 months ago)

That is a very important point and one I should have been clearer about in my response. I try to even be mindful of how much I'm spraying around when wiping down my arms and gloves. Safety is paramount with fire and explosive fumes. Thanks for pointing that out El Chupacabra!

Quote:

El Chupacabra said:
OP, just saying this since nobody else did, iso alcohol is flammable (errbody knows that tho). Some have accidentally made their SAB into arm incinerators by trapping all the alcohol fumes add flame and... BOOM!

Just wanted to share that word of caution with a newbie, don't be the next fool.

EDIT: I should have said that you can absolutely use it, and you should. Just use common sense and let the alcohol evaporate before enclosing it with your work inside and potentially your arms.




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Invisiblemaxmush
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Re: Soap or 70% iso for SAB [Re: rocklobster]
    #26883283 - 08/16/20 11:10 AM (3 years, 5 months ago)

Yes  - should have put a caution clause with iso use for noobs. Iso = flammable. Let it air out before using (usually 10min is enough but be careful)


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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OfflineYour Dudeness
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Re: Soap or 70% iso for SAB [Re: maxmush]
    #26883429 - 08/16/20 01:00 PM (3 years, 5 months ago)

Quote:

maxmush said:
Yes  - should have put a caution clause with iso use for noobs. Iso = flammable. Let it air out before using (usually 10min is enough but be careful)




Flour is flammable too.  They fight off cobweb with ISO so why not use the good stuff?


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RR sent me


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OfflineEl Chupacabra
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Re: Soap or 70% iso for SAB [Re: Your Dudeness]
    #26883570 - 08/16/20 02:33 PM (3 years, 5 months ago)

Flour isn't flammable but it does burn if it's airborne, basically everything can burn if it's capable of going airborne. It's a lot of fun to throw a handful through a bonfire


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OfflineYour Dudeness
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Re: Soap or 70% iso for SAB [Re: El Chupacabra]
    #26883624 - 08/16/20 03:12 PM (3 years, 5 months ago)

Quote:

El Chupacabra said:
Flour isn't flammable but it does burn if it's airborne, basically everything can burn if it's capable of going airborne. It's a lot of fun to throw a handful through a bonfire




Actually it's highly flammable when dispersed in air haven't you seen the news articles of flour factories going kaboom?


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RR sent me


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OfflineEl Chupacabra
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Re: Soap or 70% iso for SAB [Re: Your Dudeness]
    #26883630 - 08/16/20 03:16 PM (3 years, 5 months ago)

Right I knew what you meant when you said it. No argument here. But like I said you are referring to airborn flour.
Im sure mushrooms ground to powder is flammable in that sense too.

I like your screenname btw


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OfflineYour Dudeness
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Re: Soap or 70% iso for SAB [Re: El Chupacabra]
    #26883845 - 08/16/20 07:09 PM (3 years, 5 months ago)

Quote:

El Chupacabra said:
I like your screenname btw




Thanks man!  I've really been into the whole brevity thing and the occasional acid flashback. 

Before someone closes this thread you use soapy water to clean your equipment after use ; You use isopropy on surfaces before using the SAB.


--------------------
RR sent me


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OfflineJaydeTalbot
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Re: Soap or 70% iso for SAB [Re: Psychedelicjunkie]
    #27231040 - 02/28/21 05:56 PM (2 years, 10 months ago)

In my opinion, soap is still better. Previously, I did not give preference to soap and in general to everything I used for the body, but after the rash caused by poor-quality soap, I began to think about buying a good soap. As a result, I began to look at various sites and forums with descriptions of good products. Alaffia was my choice. I decided to try using their products and the result was really amazing, but I advise people who have not yet used their products to ask a dermatologist before using different creams, oils and shampoos. For your own health. https://www.nhc.com/brand/alaffia


Edited by JaydeTalbot (03/09/21 03:23 AM)


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OfflineShakedown Street
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Re: Soap or 70% iso for SAB [Re: JaydeTalbot]
    #27231093 - 02/28/21 06:41 PM (2 years, 10 months ago)

I set a bath towel down on the table that I know is slightly larger than my inverted SAB.    I spray the towel surface with Palmolive mixed in water in a spray bottle.
I set two wire baking racks atop the towel, so I have something to set
my agar dishes onto.
I spray that down with more Palmolive in water.    Then I spray the racks with ISO.
I set the inverted SAB onto the towel.
Next, I hold a bottle of spray Lysol into one of the armholes and spray the whole inside of the SAB.
I let that set with Saran wrap over the armholes until I get all the other tools and either agar or grain jars, or both,  prepared, along with any tools I will need to use in the SAB, like scalpel, or sterilized grain jars.  And the alcohol lamp.    And the rubber gloves sprayed with alchohol.  And the white LED that I set on top the SAB so I can see WTF I am doing.

I am on a gluten-free diet, so I have no flour in my apartment.

Please tell me if I have overlooked something important.


Edited by Shakedown Street (02/28/21 06:50 PM)


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