Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Capsules

Jump to first unread post Pages: 1 | 2  [ show all ]
OfflineUnknonw
Stranger

Registered: 09/19/17
Posts: 27
Last seen: 3 years, 4 months
Are spore syringes a crap shoot?
    #26879133 - 08/13/20 07:19 PM (3 years, 5 months ago)

I knocked up 10 WBS jars two weeks ago, and three of them are almost at the 30% colonization point. While the other 7 jars are all lifeless, except for one that has a needles worth growing. Am I doing something wrong here?


Extras: Filter Print Post Top
OfflineMad Hatter
10/6
I'm a teapot


Registered: 12/20/18
Posts: 169
Last seen: 1 year, 2 months
Re: Are spore syringes a crap shoot? [Re: Unknonw]
    #26879139 - 08/13/20 07:21 PM (3 years, 5 months ago)

Spores can take a while to germinate and spores from the same syringe will germinate at different rates. Just because they havent germinated yet doesnt mean they wont.

The reason they're consider crap shoots is that they arnt clean and you get random genetics.


Extras: Filter Print Post Top
OfflineTight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 7 days
Re: Are spore syringes a crap shoot? [Re: Unknonw]
    #26879156 - 08/13/20 07:37 PM (3 years, 5 months ago)

If you have the means to sterilize grains I suggest buying a SAB and some agar supplies. It's stupid easy and it'll allow you to grow a pile of mushrooms a bunch of different ways.


--------------------
"it's all a joke between mom contractions and coffin fittings"


The most useful tool for noobs


Extras: Filter Print Post Top
Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: Are spore syringes a crap shoot? [Re: Tight Lunchbox]
    #26879163 - 08/13/20 07:46 PM (3 years, 5 months ago)

Spore syringes can carry bacteria and even mold sometimes.
MS projects are also slow to germinate as well, which is why you should look into agar and build yourself an SAB; on the other hand you can get a flow hood which is imo the best way to go once you get better at agar.
You should do one of two things imo with a MS Syringe.
1: Make up some BRF jars and allow to fruit to obtain spore which you then start on agar.
2: Drop small droplets of spores from the syringe directly onto an agar dish to germinate.

Number one is my go to as I like to see how the fungus will fruit and grow.
I then use an SAB to collect spores and start a larger project with them .
Number two is a very common practice amongst us here and in many cases succeeds enough to yield nice plates after a few transfers.
Number two can also leave you with... lots of bacteria sometimes... too many times in my case which is the reason for number one.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


Extras: Filter Print Post Top
OfflineUnknonw
Stranger

Registered: 09/19/17
Posts: 27
Last seen: 3 years, 4 months
Re: Are spore syringes a crap shoot? [Re: mushhead]
    #26884978 - 08/17/20 02:03 PM (3 years, 5 months ago)

I ordered another syringe, as I do not have faith in 6 out of 10 of the jars. Reinject the WBS jars, or start over with fresh ones?


Extras: Filter Print Post Top
InvisibleRhizomorph
Psychedelic Researcher
Other


Registered: 04/24/20
Posts: 785
Re: Are spore syringes a crap shoot? [Re: mushhead]
    #26884996 - 08/17/20 02:13 PM (3 years, 5 months ago)

Why not MS syringe -> BRF -> cloning on agar? This seems like the best way to start out with better genetics on agar since you can pick the genetics from the best looking fruit.


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Extras: Filter Print Post Top
OfflineUnknonw
Stranger

Registered: 09/19/17
Posts: 27
Last seen: 3 years, 4 months
Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
    #26885015 - 08/17/20 02:21 PM (3 years, 5 months ago)

Wouldn't I have to first PF Tek and clone? Or no? Couldn't I just power through the wbs and clone.


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 57 minutes, 43 seconds
Trusted Cultivator
Re: Are spore syringes a crap shoot? [Re: Unknonw]
    #26885126 - 08/17/20 03:23 PM (3 years, 5 months ago)

If you have a clean syringe yes, I got four from one of the biggest vendors when I started and they were all totally clean. Later I found out it’s because they use centrifuges to separate spores which also removes contams.


You could use brf as agar, drip a tiny bit from the syringe and wait for clean myc to grow and then take that and use it to inoculate grains.


--------------------
LAGM2020


Extras: Filter Print Post Top
OfflineEntheoGod
Entheo
Male User Gallery


Registered: 03/06/15
Posts: 648
Last seen: 7 months, 27 days
Re: Are spore syringes a crap shoot? [Re: Unknonw]
    #26885167 - 08/17/20 03:46 PM (3 years, 5 months ago)

I want to respond to this with a simple.. yes.

Spore syringes are always crapshoots unlessssssss made in perfectly sterile and clean conditions.

I may sound angry 😠 lol but that’s because I have a grudge against ms syringes because of so many bad experiences when put to grain. You can get great results out of spore syringes but I recommend using agar to clean the syringe up. 😁👍


Edited by EntheoGod (08/17/20 03:47 PM)


Extras: Filter Print Post Top
InvisibleRhizomorph
Psychedelic Researcher
Other


Registered: 04/24/20
Posts: 785
Re: Are spore syringes a crap shoot? [Re: Unknonw]
    #26885254 - 08/17/20 04:23 PM (3 years, 5 months ago)

I was mainly responding to Mushhead's "second option".

You can continue with the wbs but I highly recommend at the end of your grow cloning to agar and then avoiding syringes where possible once some decent shrooms come up. You can even clone mushrooms that grow from contaminated substrate & then clean it up on agar.


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
Trusted Cultivator
Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
    #26885424 - 08/17/20 05:30 PM (3 years, 5 months ago)

i lnow i have posted this pic a fuck ton but its a great example of why to use agar and it shows that even syringes from top tier vendors are not always clean. spores themselves are dirty to some extent unless the fruits they came from were grown in an absolutely sterile environment. for the most part all spores will contain what ever contams are in the air of the environment they were grown in but this syringe came from what is if u ask me the very best vendor there is



--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
Offlineshroomrookie
truth seeker
Male User Gallery


Registered: 07/24/05
Posts: 364
Last seen: 3 years, 1 month
Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
    #26885682 - 08/17/20 08:22 PM (3 years, 5 months ago)

Quote:

Rhizomorph said:
Why not MS syringe -> BRF -> cloning on agar? This seems like the best way to start out with better genetics on agar since you can pick the genetics from the best looking fruit.




This is exactly what I do. I collect my own spores and have forever.  I've been doing brf PF tek so long. It so easy.  Then clone and done. Its easy to get some relatively clean prints to work with. I only get 1/10 contam so this is easy and convenient for me. I've skipped the agar and went straight to LC before. Once you get your clean technique down its not hard. 

If I buy a new syringe to try something new I do this. I've gotten scores of syringes from *(&&$^&#$% and never a one contam with pf brf tek. My luck I guess. Im not talking about bacteria. Bacteria doesn't stop you from cloning or printing your shrooms.

..and to tell the truth I don't get much or any bacteria either.


--------------------


Edited by shroomrookie (08/17/20 08:28 PM)


Extras: Filter Print Post Top
InvisibleRhizomorph
Psychedelic Researcher
Other


Registered: 04/24/20
Posts: 785
Re: Are spore syringes a crap shoot? [Re: jcm4620]
    #26885727 - 08/17/20 08:57 PM (3 years, 5 months ago)

Quote:

jcm4620 said:
spores themselves are dirty to some extent unless the fruits they came from were grown in an absolutely sterile environment.



I.e pins growing in vitro on agar. If somebody really wanted a sterile print/syringe they should do this.

But then why not just do agar transfers haha. I suppose if one were still using BRF cakes but I don't have the patience to wait for them to colonize :shrug:

For the sake of mailing prints/syringes though...


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
Trusted Cultivator
Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
    #26885732 - 08/17/20 08:59 PM (3 years, 5 months ago)

agar is the way to go always no matter what 👍👍


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
InvisibleRhizomorph
Psychedelic Researcher
Other


Registered: 04/24/20
Posts: 785
Re: Are spore syringes a crap shoot? [Re: shroomrookie]
    #26885735 - 08/17/20 09:00 PM (3 years, 5 months ago)

1/10 contam is a reasonably high rate of contamination if you ask some people on here. Not that my technique is much better, but both of us can definitely reduce that with better technique unless you're living in contamination central.

It does add up over time... :wink:


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Extras: Filter Print Post Top
InvisibleRhizomorph
Psychedelic Researcher
Other


Registered: 04/24/20
Posts: 785
Re: Are spore syringes a crap shoot? [Re: jcm4620]
    #26885739 - 08/17/20 09:01 PM (3 years, 5 months ago)

All hail the lord of agar :tongue2:


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
Trusted Cultivator
Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
    #26885745 - 08/17/20 09:07 PM (3 years, 5 months ago)

really u can take a steaming shit on your work table right next to your sab and as long as your methods and practices are good its not even an issue my house is trich fucking central i swear but i have a very very low contam rate so i dont think environment matters as much as good sab/fh practices matter know what im sayin

but im not sayin u should shit on ur table lol💩💩😂😂


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
OfflineCelestialexplorer1
Male


Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 2 months, 12 days
Re: Are spore syringes a crap shoot? [Re: jcm4620]
    #26885759 - 08/17/20 09:13 PM (3 years, 5 months ago)

I don’t know if it was the chocolates I ate earlier or the visual I got from reading that but it cracked me up.


--------------------


To spend just one moment in eternity


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
Trusted Cultivator
Re: Are spore syringes a crap shoot? [Re: Celestialexplorer1]
    #26885763 - 08/17/20 09:16 PM (3 years, 5 months ago)

lol glad ya get my sick sense of humor👍😂


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
Offlineshroomrookie
truth seeker
Male User Gallery


Registered: 07/24/05
Posts: 364
Last seen: 3 years, 1 month
Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
    #26885764 - 08/17/20 09:16 PM (3 years, 5 months ago)

Quote:

Rhizomorph said:
1/10 contam is a reasonably high rate of contamination if you ask some people on here. Not that my technique is much better, but both of us can definitely reduce that with better technique unless you're living in contamination central.

It does add up over time... :wink:




Maybe but Im only guessing at the 1/10. I do alot of syringes. I made one contaminated syringe back in feb.  If I use prints only a few days old they colonize super fast. Agar is the way to go for bulk grows where the loss due to contamination is high. I wouldn't want to chance bulk with MS.


--------------------


Extras: Filter Print Post Top
Offlineshroomrookie
truth seeker
Male User Gallery


Registered: 07/24/05
Posts: 364
Last seen: 3 years, 1 month
Re: Are spore syringes a crap shoot? [Re: jcm4620]
    #26885766 - 08/17/20 09:18 PM (3 years, 5 months ago)

Quote:

jcm4620 said:
really u can take a steaming shit on your work table right next to your sab and as long as your methods and practices are good its not even an issue my house is trich fucking central i swear but i have a very very low contam rate so i dont think environment matters as much as good sab/fh practices matter know what im sayin

but im not sayin u should shit on ur table lol💩💩😂😂




You're right. Good sab/fh practice is what matters. You can do miracles if you do clean work.


--------------------


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
Trusted Cultivator
Re: Are spore syringes a crap shoot? [Re: shroomrookie]
    #26885778 - 08/17/20 09:28 PM (3 years, 5 months ago)

even if u use agar thats still ms unless its a clone or an iso


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Edited by jcm4620 (08/17/20 09:29 PM)


Extras: Filter Print Post Top
Offlineshroomrookie
truth seeker
Male User Gallery


Registered: 07/24/05
Posts: 364
Last seen: 3 years, 1 month
Re: Are spore syringes a crap shoot? [Re: jcm4620]
    #26885783 - 08/17/20 09:31 PM (3 years, 5 months ago)

Quote:

jcm4620 said:
even if u use agar thats still ms unless its a clone or an iso




I'm speaking of the contamination crap shoot. And I only use agar for cloning. I'm going to do some isolation when I have some free time :smile:.


--------------------


Extras: Filter Print Post Top
OfflineCelestialexplorer1
Male


Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 2 months, 12 days
Re: Are spore syringes a crap shoot? [Re: shroomrookie]
    #26885800 - 08/17/20 09:46 PM (3 years, 5 months ago)

Why not try no pour agar teks that’s what I do because I have a small sab no flow hood and I know my vents in my house are probably the culprit in the contams floating around my house so I do no pour and now my cult success rate is so crazy high from where it used to be. If you have a PC and you should then all you need is the nutes the agar and the cups on amazon are 50 for 14$. You can get everything you need for a total of $50 your probably wasting that on grain alone and my time is worth $50.


--------------------


To spend just one moment in eternity


Extras: Filter Print Post Top
InvisibleRhizomorph
Psychedelic Researcher
Other


Registered: 04/24/20
Posts: 785
Re: Are spore syringes a crap shoot? [Re: jcm4620]
    #26885923 - 08/18/20 12:34 AM (3 years, 5 months ago)

I'm at the point where if a TC told me that shitting on my table next to my SAB would improve my grow I'd probably try it.


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Extras: Filter Print Post Top
Invisiblegeorge castanzaM
Lord Of The Idiots!
Male User Gallery

Registered: 10/21/02
Posts: 8,721
Trusted Cultivator
Re: Are spore syringes a crap shoot? [Re: Unknonw]
    #26885954 - 08/18/20 01:29 AM (3 years, 5 months ago)

Quote:

Unknonw said:
I ordered another syringe, as I do not have faith in 6 out of 10 of the jars. Reinject the WBS jars, or start over with fresh ones?




Definitely start over with fresh jars.




Properly sourced spore syringes of well known stable varieties produce consistent results with consistent techniques.

I believe most of the hype surrounding the "crap shoot" is inconsistent growers getting inconsistent results, then learning more through practice, growing an isolate or some clean spawn on agar, that they take a lot more time and energy caring for,  then wind up getting better results and credit it more to agar work than consistent correct practical methods of preparing substrates to fruit.  Of course I have been on a very long run of clean vendor syringes so my opinion is likely jaded from my own personal experience. Also, if I am making syringes myself I like to run a hepa in the area while the mushrooms are popping for the prints.

In essence life itself is a crap shoot and since you can find reliably in agar cultures, spore syringe's reliability is sometimes under valued. Fear not and be hopeful that your jars will bring fruits in abundance.




Two weeks with no growth whatsoever certainly suggest something is up, especially with the other jars already being at 30%. Maybe the jars got dried out somehow?  With the information available dried jars or bacterial contamination would be my guess at the culprit.

Pictures and more details would help.
Temps? Tek? Strain?

Quote:

Unknonw said:
Couldn't I just power through the wbs and clone.




Yes.


--------------------
KRAMER CAKES




Extras: Filter Print Post Top
Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
    #26886426 - 08/18/20 10:22 AM (3 years, 5 months ago)

Quote:

Rhizomorph said:
I'm at the point where if a TC told me that shitting on my table next to my SAB would improve my grow I'd probably try it.




Lol! Rihizomorth I feel ya. It took me a while before i was able to clean prints and syringes on agar. (i am still having some issues but i have narrowed it down to the prints/syringes themselves and not my sterile tek). They are inherently contaminated to start.

Dilution streaking teks are your best bet and use more plates to start off so you have a better selection.

The tek i found that works best (for me) is using a sterilized q-tip as I found i had much more control over the streaking and also picked up the spores better than an inoculation loop.


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Capsules


Similar ThreadsPosterViewsRepliesLast post
* spore syring is in trouble? mulan 14,122 12 08/29/09 09:15 PM
by HaRo On KoRn
* Spore Syring Cont/Clear Misconcept if any Zwieback0 5,674 4 08/11/08 03:22 AM
by fr33d0mfry
* Re: When buying a spore syring... 3Mshroom 7,932 2 06/01/00 08:17 PM
by 3Mshroom
* Re: malt water + spores = monocariotic crap? Shroomzilla 565 2 04/03/01 03:22 AM
by sylo
* how far to spore prints go? AKA PC 3,737 11 01/28/03 08:07 AM
by debianlinux
* Re: Spore transfer? Anonymous 1,901 1 03/08/01 08:17 PM
by Juggles
* spores userdoesnotexist 1,508 5 08/06/02 07:52 AM
by mickey_rourke
* How long do spore syringes last? SixCee 5,255 4 08/01/02 10:39 PM
by mickey_rourke

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
610 topic views. 29 members, 187 guests and 29 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.01 seconds on 14 queries.