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Offlineshroomrookie
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Registered: 07/24/05
Posts: 364
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Re: Are spore syringes a crap shoot? [Re: jcm4620]
    #26885766 - 08/17/20 09:18 PM (3 years, 5 months ago)

Quote:

jcm4620 said:
really u can take a steaming shit on your work table right next to your sab and as long as your methods and practices are good its not even an issue my house is trich fucking central i swear but i have a very very low contam rate so i dont think environment matters as much as good sab/fh practices matter know what im sayin

but im not sayin u should shit on ur table lol๐Ÿ’ฉ๐Ÿ’ฉ๐Ÿ˜‚๐Ÿ˜‚




You're right. Good sab/fh practice is what matters. You can do miracles if you do clean work.


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Offlinejcm4620
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Registered: 05/26/19
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Re: Are spore syringes a crap shoot? [Re: shroomrookie]
    #26885778 - 08/17/20 09:28 PM (3 years, 5 months ago)

even if u use agar thats still ms unless its a clone or an iso


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PANAEOLUS FRUITING MADE SIMPLE



Edited by jcm4620 (08/17/20 09:29 PM)


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Offlineshroomrookie
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Registered: 07/24/05
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Re: Are spore syringes a crap shoot? [Re: jcm4620]
    #26885783 - 08/17/20 09:31 PM (3 years, 5 months ago)

Quote:

jcm4620 said:
even if u use agar thats still ms unless its a clone or an iso




I'm speaking of the contamination crap shoot. And I only use agar for cloning. I'm going to do some isolation when I have some free time :smile:.


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OfflineCelestialexplorer1
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Registered: 03/25/20
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Re: Are spore syringes a crap shoot? [Re: shroomrookie]
    #26885800 - 08/17/20 09:46 PM (3 years, 5 months ago)

Why not try no pour agar teks thatโ€™s what I do because I have a small sab no flow hood and I know my vents in my house are probably the culprit in the contams floating around my house so I do no pour and now my cult success rate is so crazy high from where it used to be. If you have a PC and you should then all you need is the nutes the agar and the cups on amazon are 50 for 14$. You can get everything you need for a total of $50 your probably wasting that on grain alone and my time is worth $50.


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To spend just one moment in eternity


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InvisibleRhizomorph
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Registered: 04/24/20
Posts: 785
Re: Are spore syringes a crap shoot? [Re: jcm4620]
    #26885923 - 08/18/20 12:34 AM (3 years, 5 months ago)

I'm at the point where if a TC told me that shitting on my table next to my SAB would improve my grow I'd probably try it.


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:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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Invisiblegeorge castanzaM
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Re: Are spore syringes a crap shoot? [Re: Unknonw]
    #26885954 - 08/18/20 01:29 AM (3 years, 5 months ago)

Quote:

Unknonw said:
I ordered another syringe, as I do not have faith in 6 out of 10 of the jars. Reinject the WBS jars, or start over with fresh ones?




Definitely start over with fresh jars.




Properly sourced spore syringes of well known stable varieties produce consistent results with consistent techniques.

I believe most of the hype surrounding the "crap shoot" is inconsistent growers getting inconsistent results, then learning more through practice, growing an isolate or some clean spawn on agar, that they take a lot more time and energy caring for,  then wind up getting better results and credit it more to agar work than consistent correct practical methods of preparing substrates to fruit.  Of course I have been on a very long run of clean vendor syringes so my opinion is likely jaded from my own personal experience. Also, if I am making syringes myself I like to run a hepa in the area while the mushrooms are popping for the prints.

In essence life itself is a crap shoot and since you can find reliably in agar cultures, spore syringe's reliability is sometimes under valued. Fear not and be hopeful that your jars will bring fruits in abundance.




Two weeks with no growth whatsoever certainly suggest something is up, especially with the other jars already being at 30%. Maybe the jars got dried out somehow?  With the information available dried jars or bacterial contamination would be my guess at the culprit.

Pictures and more details would help.
Temps? Tek? Strain?

Quote:

Unknonw said:
Couldn't I just power through the wbs and clone.




Yes.


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KRAMER CAKES




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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: Are spore syringes a crap shoot? [Re: Rhizomorph]
    #26886426 - 08/18/20 10:22 AM (3 years, 5 months ago)

Quote:

Rhizomorph said:
I'm at the point where if a TC told me that shitting on my table next to my SAB would improve my grow I'd probably try it.




Lol! Rihizomorth I feel ya. It took me a while before i was able to clean prints and syringes on agar. (i am still having some issues but i have narrowed it down to the prints/syringes themselves and not my sterile tek). They are inherently contaminated to start.

Dilution streaking teks are your best bet and use more plates to start off so you have a better selection.

The tek i found that works best (for me) is using a sterilized q-tip as I found i had much more control over the streaking and also picked up the spores better than an inoculation loop.


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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