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OfflineFietchen
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Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Second Attempt with B+Cubensis. Wish me Luck!
    #26878658 - 08/13/20 02:04 PM (3 years, 5 months ago)

Hi, i have done some Shit last week and try to make my own think and try to make my own Mycelium TEK with Rye and Coco total Stupid not doing the simple think so my Faul!

This time i research and informing me best i can. Yesterday my Syringe Arrived with another B+ Strain. My Fear this time bevore the Syringe arrived are the Temps we have here for the last 7 Days. 30 degrees + in the shadow so the Syringe are arrived at this Temps and i have put the Syringe in the Fridge to today. Yesterday i have starting first the Rye Jar TEK letting the Rye beans sitting in water for 12-24 Hours and Boiling them again for 15 Minutes, than i let them Cool down and do the Paper Trick to test the Humidity no wet spots on the paper and i make my Jars ready today with a litte hole and some "Viskose" to protect the holes. The fear are know the Polyfill i have thought it is Polyfil but it doesn't ;( but i have done this last year too with my Oyster mushroom and all goes well so hopefully it's going well to know without this Polyfill stuff.

Today i shoot the Spores in my 3 Jars in my bathroom Spraying Alcohol all around and make my jars steril and the Syringe. In every corner of the Jar i shoot the Spores in hopefully not to much that's piss me of that i shoot 3ml in 3 jars :frown: maybe to much what you think? Good News are i can make new Jars and i will tomorrow so better safe than sorry. I bring my Jars in my Room downstairs the temperature there are 20-23 celsius better than 30 here today.

How good are the chance know for success? Ore i doing shit again with to much ml per Jar?


That how it looks



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OfflineRapjack
Oat Soakin' Toker
I'm a teapot

Registered: 05/15/17
Posts: 483
Loc: Elsewhere
Last seen: 2 years, 3 months
Re: Second Attempt with B+Cubensis. Wish me Luck! [Re: Fietchen]
    #26878728 - 08/13/20 02:36 PM (3 years, 5 months ago)

Spores to grain can work but it's not reliable. Spores are always contaminated because they're grown in open air. Grains are highly nutritious which contaminants love. Personally I'd recommend injecting some of your solution into PF TEK cakes instead. Then if you want to go further build a Still Air Box and look into agar while they colonize.

At the very least doing this in a SAB will greatly increase your chance of success.


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OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: Second Attempt with B+Cubensis. Wish me Luck! [Re: Rapjack]
    #26878742 - 08/13/20 02:42 PM (3 years, 5 months ago)

Thanks I will make a liquid culture on agar to but maybe my jars are healthy. It is bad to shoot 3-4ml in 1 jar? Maybe it colognising quicker. I have 10ml spores left so new jar i will try and some agar i will make too.


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OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: Second Attempt with B+Cubensis. Wish me Luck! [Re: Fietchen]
    #26891295 - 08/21/20 08:44 AM (3 years, 5 months ago)





I loosing 1 of my 6 jars :frown: this Jar was the one i Injecting to much spores 3ml stupidly pay the price. But I make 3 new day later with 1ml per Jar hopefully everything is OK. I make a liquid culture also for safety reason and later on try to make the rest of my syringe.


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