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trippytimes
♡contamination queen♡



Registered: 09/02/14
Posts: 1,097
Last seen: 1 year, 3 months
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Laminar flowhood contamination
#26877952 - 08/13/20 06:37 AM (3 years, 6 months ago) |
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So I have had this flow hood since I started here back 5+ years ago. These last two months. I have been having horrible contamination on my agar and g2g. I have ruled out my sterile technique as I have been very successful in the last 4 years. I have ruled out the fact that D auger plates I've been using has contamination. Do you have to replace the HEPA filter on these things after so many hours of use? That's the only thing I can think of as I use a TV suit mask non-powder coated gloves rubbing alcohol for days and I let my flow hood run for 30 minutes prior to using it.
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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Re: Laminar flowhood contamination [Re: trippytimes]
#26877960 - 08/13/20 06:50 AM (3 years, 6 months ago) |
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Filters do need to be replaced. I've seen people say they last anywhere from 3 to 10 years. Haven't had to replace mine yet, so I can't say from personal experience. Do you use a prefilter?
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trippytimes
♡contamination queen♡



Registered: 09/02/14
Posts: 1,097
Last seen: 1 year, 3 months
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Yes I do. It's a HEPA prefilter that I buy from Home Depot a 12x12x1 to go behind the dayton blower.I've already changed that I just don't want to have to change my HEPA filter because that was a good $300 https://filterbuy.com/air-filters/12x12x1/merv-13/?utm_source=google&utm_medium=pla&utm_campaign=google%20pla&gclid=CjwKCAjwydP5BRBREiwA-qrCGivlnhlhi95n7BGv18XadD4WcAZ6jmHOvxXS4nSpGMfcdCtHopW_ZxoC0S4QAvD_BwE#1-pack it's not a Merv 13 though it's like a Merv 8 prefilter
Edited by trippytimes (08/13/20 07:17 AM)
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
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Re: Laminar flowhood contamination [Re: trippytimes]
#26877975 - 08/13/20 07:14 AM (3 years, 6 months ago) |
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Do an open plate test. If the plates go bad, it's more than likely your filter. It will either need fixed or replaced. Also, check the flow coming out of the face of the filter. Could be getting clogged up and the flow is no longer high enough
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trippytimes
♡contamination queen♡



Registered: 09/02/14
Posts: 1,097
Last seen: 1 year, 3 months
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Re: Laminar flowhood contamination [Re: Gan]
#26877982 - 08/13/20 07:19 AM (3 years, 6 months ago) |
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I did the lighter test and it did pretty good the flame was almost bent at a 90° but not going out. I am going to take an agar dish and put it in front of it for 60 seconds and then close it up and wait a week before I transfer all these grains again this sucks I might have to break out the old sab. Edit sorry for my grammar slaughter trying to do this at work underneath the desk.
Edited by trippytimes (08/13/20 07:33 AM)
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LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Re: Laminar flowhood contamination [Re: trippytimes]
#26878107 - 08/13/20 09:13 AM (3 years, 6 months ago) |
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Be glad it lasted you that long. It is filter replacing time for you. So accept it and get 'er done.
Happened to me. My first diy 2x2 lasted a few years with no prefilter. Then my success rate tanked hard and fast. Wanted to blame myself and not equipment. Ruled out everything and it was my filter. Decided to upgrade vs just replacing the old filter.
90° is too much of an angle. Fluttering around 45°-60° is more ideal.
Want real info you can work with? Get an anemometer.
Edited by LotKid (08/13/20 09:15 AM)
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trippytimes
♡contamination queen♡



Registered: 09/02/14
Posts: 1,097
Last seen: 1 year, 3 months
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Re: Laminar flowhood contamination [Re: LotKid]
#26878196 - 08/13/20 10:07 AM (3 years, 6 months ago) |
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I guess it is time for a new one. And I'm going to have to look that up I've never heard of one. Are they expinsive? Also I don't want to start another thread so if someone can chime in on this it'd be great. I'm trying to "study" Panaeolus cyanescens again but recently I have moved and do not have access to hpoo but I don't see anybody "studying" off of Coco I know this is a dung species And usually I have just used hpoo. Any thoughts?
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micelio
Song of Silence


Registered: 04/22/14
Posts: 1,547
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Re: Laminar flowhood contamination [Re: trippytimes]
#26878226 - 08/13/20 10:31 AM (3 years, 6 months ago) |
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trippytime...
I had to move a few years ago. The movers grab the filter with their fingers after I told them not to touch the filter.
When I ran the filter for the first time after the move I notice allot more air movement on the side where the mover touch the filter but I couldn't visually see any damage.
I took the back panel off the flow hood and notice the filter had a three inch rip going up and down.
So now I have a sheet of veneer I screw on over the filter If the flow hood needs to be moved.
I was able to patch the rip with flex seal, I imagine silicone would of worked. So now I have a dead area but It doesn't seem to effect the performance much..
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trippytimes
♡contamination queen♡



Registered: 09/02/14
Posts: 1,097
Last seen: 1 year, 3 months
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Re: Laminar flowhood contamination [Re: micelio]
#26878249 - 08/13/20 10:42 AM (3 years, 6 months ago) |
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That what might have happened.... Hmmmmm.... I did hire movers to move everything and had to stop everything except my oysters. I might have to have my bf to check the inside because I have a new prefilter but the main filter is over 4 years old And I use it almost daily with g2g and agar. My dishes looked like someone painted in them for all the different contams ugg IV lost my first new batch since IV moved and that was alot 30 jars total and 10 plates. IV tryed everything so it has to be the main filter even cleaned and sterilized the whole room with bleach and water. Never had a issue before I moved even moved the hood to a new room to see if maybe it was the room.
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Laminar flowhood contamination [Re: LotKid]
#26878280 - 08/13/20 10:54 AM (3 years, 6 months ago) |
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Quote:
LotKid said: Get an anemometer quality anemometer.
Fixed it for ya 
Cheap anemometers suck balls. You need a nice one that costs dozens of monies not just 10 monies
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trippytimes
♡contamination queen♡



Registered: 09/02/14
Posts: 1,097
Last seen: 1 year, 3 months
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Re: Laminar flowhood contamination [Re: natedawgnow]
#26878364 - 08/13/20 11:38 AM (3 years, 6 months ago) |
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Lmfao you guys are crazy yeah I just Google searched it and they're like 3 to $500. hmmm looks like I need a sugar daddy that can help loan one. I'll send pictures of my feet lol. Any takers pm me I'll send it back. Or if your local to ATL ga. Really wouldn't mind paying for gas to have someone check it out before I drop $375 for a new HEPA. But I feel like it needs to be done. I have used it for few hundred hours at this point I'm pretty sure.
Edited by trippytimes (08/13/20 11:41 AM)
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LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Re: Laminar flowhood contamination [Re: natedawgnow]
#26878366 - 08/13/20 11:39 AM (3 years, 6 months ago) |
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C'mon nate... coming from me it should be already assumed i'm talking bout the good shit!
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LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Re: Laminar flowhood contamination [Re: trippytimes]
#26878368 - 08/13/20 11:41 AM (3 years, 6 months ago) |
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Quote:
trippytimes said: Lmfao you guys are crazy yeah I just Google searched it and they're like 3 to $500. hmmm looks like I need a sugar daddy that can help loan one. I'll send pictures of my feet lol. Any takers pm me I'll send it back.
I'm more of a back of the knee and inside of the elbow kinda guy... 
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micelio
Song of Silence


Registered: 04/22/14
Posts: 1,547
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Re: Laminar flowhood contamination [Re: trippytimes]
#26878386 - 08/13/20 11:51 AM (3 years, 6 months ago) |
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Quote:
trippytimes said: That what might have happened.... Hmmmmm.... I did hire movers to move everything and had to stop everything except my oysters. I might have to have my bf to check the inside because I have a new prefilter but the main filter is over 4 years old And I use it almost daily with g2g and agar. My dishes looked like someone painted in them for all the different contams ugg IV lost my first new batch since IV moved and that was alot 30 jars total and 10 plates. IV tryed everything so it has to be the main filter even cleaned and sterilized the whole room with bleach and water. Never had a issue before I moved even moved the hood to a new room to see if maybe it was the room.
My opinion.. You should have no problem getting allot more years If your running a pre-filter.
I remember R.R. saying he was on his tenth year with a pre-filter.
When the back Is off your flow hood vacuum your filter with a brush attachment.
the flame on a bic lighter should blow out If there's any damage to the filter.
Also If there's damage you could operate on the opposite end of the damage. If you work nice and tight close to the filter face, just to get by..
Please Let us know the outcome of you situation..
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Laminar flowhood contamination [Re: LotKid]
#26878420 - 08/13/20 12:17 PM (3 years, 6 months ago) |
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Quote:
LotKid said: C'mon nate... coming from me it should be already assumed i'm talking bout the good shit! 
Not everybody is a classy mother fucker like you, lot
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trippytimes
♡contamination queen♡



Registered: 09/02/14
Posts: 1,097
Last seen: 1 year, 3 months
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Re: Laminar flowhood contamination [Re: micelio]
#26878483 - 08/13/20 12:55 PM (3 years, 6 months ago) |
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It does have a prefilter I really do think they dropped it or something when I moved. Funny it is messing up when I just moved.... By the way I'm still trying to figure out if Coco is ok with p cyans. I'm wanting to regrow them again and I don't have a supply of HP around me that I know of especially since I'm new around the area.has anybody had any success or do I have to go buy bags of manure?
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LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Re: Laminar flowhood contamination [Re: natedawgnow]
#26878608 - 08/13/20 01:45 PM (3 years, 6 months ago) |
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Quote:
natedawgnow said:
Quote:
LotKid said: C'mon nate... coming from me it should be already assumed i'm talking bout the good shit! 
Not everybody is a classy mother fucker like you, lot 
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