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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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OfflineKuroNeko
Mycelium Tycoon
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Registered: 05/19/20
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Loc: North Korea
Last seen: 7 months, 3 days
Question about agar transfers and agar to grain of overgrown plates.
    #26876683 - 08/12/20 10:45 AM (3 years, 5 months ago)

Couple of questions. How important is to transfer leading edge of mycelium branch? I left my nice cultures for too long and they grew to the edge and up the walls. Is it fine to cut a piece from a good branch little behind the edge? Will it be fine or subpar and need to start over again? Same thing with agar to grain. Is there difference which part of fully colonised isolated plate I cut out and put to grain, I mean from center to edge? Does it matter at all or science tells us to use only pieces furthest from center?


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English not my native language, please excuse grammar.


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Offlineredhandmat
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Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Question about agar transfers and agar to grain of overgrown plates. [Re: KuroNeko]
    #26876690 - 08/12/20 10:52 AM (3 years, 5 months ago)

When cleaning up the culture its very important to do so. But In my personal experience, once you get a few transfers after the initial germination and you have had clean growth for a while then its fine even when the plate is overgrown. I have used clean overgrown plates and its been fine to take to grains and then coir and never seen a hint of contam.

In summary, the edge is more important when cleaning up and isolating the fast growing part of the myc.


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OfflineKuroNeko
Mycelium Tycoon
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Registered: 05/19/20
Posts: 150
Loc: North Korea
Last seen: 7 months, 3 days
Re: Question about agar transfers and agar to grain of overgrown plates. [Re: redhandmat]
    #26876723 - 08/12/20 11:16 AM (3 years, 5 months ago)

Yeah, I know about cleaning and I'm not asking about contamination risk at all. I'm wondering if taking 'earlier' piece of growth would be 'degraded' in any way so to speak. Or it doesn't matter at all what chronological piece of same sector we take to another plate or grain?


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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