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Offlinejbgtaa
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Re: Psilocybe natalensis [Re: Nichrome]
    #26874868 - 08/11/20 11:20 AM (3 years, 6 months ago)

How would one prove ot? I dont know much about crossing.


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OfflineNichrome
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Re: Psilocybe natalensis [Re: jbgtaa]
    #26874926 - 08/11/20 11:41 AM (3 years, 6 months ago)

Would need a better microscope rigged up and individual isolation's to watch. With the naked eye at this magnification (much clearer and wider focus than the camera)you can barely see the outline of clamps happening but it's not for sure. At better magnification you could clearly see clamps happening. The dish could be opened up and a cover slip placed on the surface to get a closer view, but this is the best with the current setup through a sealed petri dish. The cover slip also ruins any 3D viewing at different focal depths to show areal growth habit. Once there is mycelium to spare I'll try for some better shots.

It does not at this point seem to be competitive or adverse to the cube mycelium whether or not it is trying to breed.


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: Nichrome]
    #26875147 - 08/11/20 01:48 PM (3 years, 6 months ago)

Good thing you didn’t toss that crack those are some fatties. They took over a month to see pins right??


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Offlineshroomflow
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Re: Psilocybe natalensis [Re: Nichrome]
    #26875182 - 08/11/20 02:04 PM (3 years, 6 months ago)

Quote:

Nichrome said:
Watching this behave under the microscope is intriguing. I think it is a cube.




This is my theory as well, although I have yet to look at it under the scope. It's a cube that has some unusual adaptions. The fruit grown outdoors are indistinguishable from cubes grown outdoors, at least as far as I can tell.


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Offlinejbgtaa
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Re: Psilocybe natalensis [Re: shroomflow]
    #26875374 - 08/11/20 03:56 PM (3 years, 6 months ago)

Veil remnant, annulus, and gill attachment are usually species dividing characteristics when microscopy is unavailable. I wouldnt be surprised if it was similar to PE in the sense that it mutated for different potency and characteristics due to the locale. I just don't see how those minute differences would be advantageous to a fungus if it were to separate from the original phenotype.


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OfflineNichrome
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Re: Psilocybe natalensis [Re: jbgtaa]
    #26875419 - 08/11/20 04:17 PM (3 years, 6 months ago)

This is a cube from a wild outdoor print I collected in Costa Rica. Note the gill attachment and lack of veil. These fruits were not hollow at all. The caps separated from the stem when they were less than an inch tall.


First generation from wild spores.


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OfflineNichrome
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Re: Psilocybe natalensis [Re: jbgtaa]
    #26875429 - 08/11/20 04:19 PM (3 years, 6 months ago)

Quote:

jbgtaa said:
Veil remnant, annulus, and gill attachment are usually species dividing characteristics when microscopy is unavailable. I wouldnt be surprised if it was similar to PE in the sense that it mutated for different potency and characteristics due to the locale. I just don't see how those minute differences would be advantageous to a fungus if it were to separate from the original phenotype.




The entertainment of a caretaker giant species is an advantageous adaptation indeed. Seems conscious no?


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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: Nichrome]
    #26875537 - 08/11/20 05:17 PM (3 years, 6 months ago)

Is that the playa uvita?? I saw pics of it the other day and it’s the only thing that looks similar to the natalensis I’ve been getting with the long stipes and smaller caps. The playa I grew had a ton of phenos though it’s one of my favorites.

Even those don’t have the gills that run down the stipe a quarter inch or so though.

That pic isn’t as good as the one somebody put up a couple pages back.

That combined with the potency and how finicky it is makes me think it’s gotta be something different. Maybe related but from what I’ve seen the only thing cube about it is the coloring.

Definitely can’t wait for the lab results.


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Edited by A.k.a (08/11/20 05:20 PM)


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OfflineNichrome
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Re: Psilocybe natalensis [Re: A.k.a]
    #26875555 - 08/11/20 05:26 PM (3 years, 6 months ago)

Yes that's the Playa (no proof the Playa is a cube either lol). Yeah I see where you are coming from. I think it's a cube but really on the fence about it. Knowing that there is genetic testing in the works whelms me greatly.

LC is looking good to try a run next week. Anybody using pasteurized chopped straw or is everybody going coco or poo? I love straw for the cubes. Probably won't go back to coco. Any different results with different subs?


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Better to be deprived of food for three days, than tea for one.


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OfflinePsiloPsychIn
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Re: Psilocybe natalensis [Re: Nichrome] * 1
    #26875763 - 08/11/20 06:59 PM (3 years, 6 months ago)

This is the same culture that gave me a canopy.
A couple of days ago:



Today:



A sweet looking plate pin clone from that culture:



And just a plate of it:



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OfflineA.k.aM
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Re: Psilocybe natalensis [Re: PsiloPsychIn]
    #26875795 - 08/11/20 07:23 PM (3 years, 6 months ago)

Awesome, that myc is much different than the plates I’ve been running.

What type of agar recipe do you use?


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OfflineNichrome
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Re: Psilocybe natalensis [Re: A.k.a]
    #26875849 - 08/11/20 08:05 PM (3 years, 6 months ago)

Beautiful.


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Re: Psilocybe natalensis [Re: Nichrome] * 1
    #26875921 - 08/11/20 09:11 PM (3 years, 6 months ago)

3.75g fresh in some lemon/green tea had about the effect of an eighth dried cubensis. The tub i posted a few pages back has been just flushing out dry quarters every 5 or so days. Its got a huge patch of green mold so I think its about done


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OfflineGrimsweeper
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Re: Psilocybe natalensis [Re: jbgtaa]
    #26876109 - 08/11/20 11:53 PM (3 years, 6 months ago)

That culture looks awesome psilo. The one i have going from ms now isn't like any I had before so I'm hoping for good things ahead.

6 days in on a CV mono



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Edited by Grimsweeper (08/11/20 11:58 PM)


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OfflinePsiloPsychIn
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Re: Psilocybe natalensis [Re: A.k.a]
    #26876211 - 08/12/20 01:54 AM (3 years, 6 months ago)

Quote:

A.k.a said:
Awesome, that myc is much different than the plates I’ve been running.

What type of agar recipe do you use?




Most of the time I just use MEA sometimes I mix it up and do potato flakes malt and agar.
To be honest I can’t recall this time.. I suspect MEA


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Re: Psilocybe natalensis [Re: PsiloPsychIn]
    #26876334 - 08/12/20 06:00 AM (3 years, 6 months ago)

Any idea if the plates are high or low nute?

This is my clone that I split the jar of spawn, coir on 8/3 manure on 8/6.



Idk what’s going on with the coir but it’s really liking the manure.

The coir tub has half the usual spawn with the same amount of coir, the manure tub is like an inch deep so it’s got a normal ratio.


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Edited by A.k.a (08/12/20 06:01 AM)


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OfflinePsiloPsychIn
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Re: Psilocybe natalensis [Re: A.k.a] * 1
    #26876366 - 08/12/20 06:35 AM (3 years, 6 months ago)

My MEA recipe is 10 grams Extra Light Malt, 10 grams agar, to  500ml 1000ml water. I usually end up going a little less on malt or a little more on water ...

As long as we are tracking things...
That tub is 4 full quarts spawn to 6 quarts coir verm.  I thoroughly Mixed 4 quarts spawn with 4 quarts coir verm, evened it out and added a layer of two quarts coir verm on top and leveled it out.


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Edited by PsiloPsychIn (08/13/20 08:21 PM)


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OfflinePsiloPsychIn
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Re: Psilocybe natalensis [Re: PsiloPsychIn]
    #26879182 - 08/13/20 08:11 PM (3 years, 6 months ago)

Ok was hoping I’d be seeing some pins by now but - NOPE!
I’ve got the top off completely and the RH in the Martha is about 75-85% , fans periodically move air around.  I did notice a similar growth to that weird thing that was in my last tub that was massive in the end:



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OfflineGrimsweeper
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Re: Psilocybe natalensis [Re: PsiloPsychIn]
    #26879201 - 08/13/20 08:22 PM (3 years, 6 months ago)

Still looks pretty good psilo. My last culture would've been overun by blobbyness by then. Are the sides and bottom fully colonized?


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Re: Psilocybe natalensis [Re: Grimsweeper]
    #26879525 - 08/14/20 03:51 AM (3 years, 5 months ago)

My guess is yes. I have a black plastic liner so I’m not sure what is going on in there. It just occurred to me tonight that the flush happened while I was away for four days... and I always drop the temp down to 65 or even 60F when I’m away to keep things from drying out... I may drop the temp and see if that stimulates pinning


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