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Offlinelookintolearn
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Registered: 06/02/20
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Re: Pastywhyte's Easy Agar Tek [Re: A.k.a]
    #26841313 - 07/23/20 06:59 PM (3 years, 6 months ago)

Thanks for the replies guys :thumbup: okay so room temp would work for at least a month I can probably make that happen since I only poured a few.

Now i'm at a different problem so just a minute ago I went to go take the plates out and there is quite a bit of condensation on the sides. I did a fair bit of reading and have come to the conclusion that flipping the cups over is the best course of action although I have some questions about this.

I had covered the cups as per tek with paper towels and foil but decided to take them off outside the SAB and put the plates in there upside down. I fear the condensation would ruin my micropore tape and let contams in. Is this a valid fear I have? I have seen many people say flicking the cup would help but to me that sounds like...bullshit? I think all that would do is cause more water buildup on the agar which isn't preferable.

Was taking the papertowel and foil off a smart move? They are sitting like that right now and I want to inoculate tonight if possible.


--------------------
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Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: lookintolearn]
    #26841329 - 07/23/20 07:04 PM (3 years, 6 months ago)

I only work with my pasty plates upside down :wink:

heres a vid of transfers upside down



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Offlinelookintolearn
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #26841398 - 07/23/20 07:40 PM (3 years, 6 months ago)

It's funny that you replied Munch I was just reading a conversation you had with Josex from awhile back about this which made me think it was the best route  :cookiemonster: So what do you think about the condensation though? Is it best to just let it sit a few nights upside down? Dump out the little thats in there? Or just go ahead as long as its not a whole swimming pool in the agar?


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: lookintolearn] * 1
    #26841426 - 07/23/20 07:54 PM (3 years, 6 months ago)

just dump it in the sab when you goto make a transfer


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Offlinelookintolearn
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #26841749 - 07/23/20 11:08 PM (3 years, 6 months ago)

You're a good man don't let no one tell you different  :rockon: Thanks a bunch munch


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


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InvisibleDarkshadow
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Registered: 06/11/20
Posts: 55
Re: Pastywhyte's Easy Agar Tek *DELETED* [Re: Pastywhyte]
    #26841947 - 07/24/20 03:14 AM (3 years, 6 months ago)

Post deleted by Darkshadow

Reason for deletion: .


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OfflineKiloecho
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Re: Pastywhyte's Easy Agar Tek [Re: Darkshadow]
    #26848837 - 07/27/20 09:13 PM (3 years, 6 months ago)

Thanks for this TEK! I’m going to try this next round of agar.


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Offlinehowdidigethere
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Re: Pasty Agar Tek [Re: Kiloecho]
    #26873335 - 08/10/20 01:16 PM (3 years, 6 months ago)

Many many thanks!!



HDIGH


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Invisiblepablokabute
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #26874424 - 08/11/20 04:18 AM (3 years, 6 months ago)

Quote:

Munchauzen said:
just dump it in the sab when you goto make a transfer





If you flip your pp container, all the condensation and agar "juices" go to the edges, it could get inoculated with contaminant spores while sitting around like that or become another vector of contam when youre finally ready to make a transfer and open your container.


--------------------

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Offlinefalconman
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Re: Pastywhyte's Easy Agar Tek [Re: pablokabute]
    #26879752 - 08/14/20 08:40 AM (3 years, 6 months ago)

Ok I have(I think) a new issue. cracked(like literally) lids. I couldnt find the glad rounds so I used some small rubbermaid containers. 4 ready to go, 1/4 inch holes in the lids with MP tape, completely wrapped in foil, and PC'd for 42 minutes. Feft overnight to cool, and when I opened them up today, all 4 lids have 1-2" long cracks in the top. Anyone else run into this and know why it happens/a solution?

I ran some more MP tape over the cracks and tried to inoculate anyway, but my hopes arent high


--------------------
If any of my posted info is incorrect or questionable,please DO correct me. I strive to always learn new or better ways and information




Grower off all things nature!
things currently growing I am proud of:
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InvisibleMadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
Re: Pastywhyte's Easy Agar Tek [Re: falconman]
    #26879761 - 08/14/20 08:54 AM (3 years, 6 months ago)

When looking for containers you want to make sure they are PP5 plastic. The containers may have been PP5 but the lids maybe not. They could still grow some healthy mycelium though depending on if they were exposed to the air or how big the cracks were. If they weren’t open cracks you may be good. I’ve never had that issue though.


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Offlinefalconman
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Re: Pastywhyte's Easy Agar Tek [Re: MadHatter333]
    #26879807 - 08/14/20 09:28 AM (3 years, 6 months ago)

yep, containers at least are pp5, no markings on lids tho. they were open cracks and a few mm wide, but once they were out of the PC, they were in the SAB and got taped right after unfoiling.

we'll see what happens


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InvisibleMadHatter333
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Re: Pastywhyte's Easy Agar Tek [Re: falconman]
    #26879834 - 08/14/20 09:47 AM (3 years, 6 months ago)

Should be fine :goodluck: you can always start over too.


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OfflineMadHatR
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Re: Pastywhyte's Easy Agar Tek [Re: MadHatter333]
    #26895712 - 08/23/20 05:02 PM (3 years, 5 months ago)

I have read through over 200 replies and still haven't found a answer, so I guess I will just ask.

Has anyone used the Ziploc Mini's, or any other form of Pasty Plates to inject a spore syringe through the lid to germinate the spores? This could be through the process of modifying the lid and adding a SHIP by adding a injection port or making one by squeezing RTV Silicone through the hole, flattening it on both sides and then letting it cure for a day. You could also in theory just plunge the needle through the micro-pore tape, inject spores, then remove and put more tape over the hole. You could even slide the needle through the Poly-Fil.

As a follow up could the opposite also be done?

-Knock up several plates.
-Wait for germination and growth.
-Take the best ones and stick a clean 14 gauge biopsy needle through the SHIP.
-Scoop up a sample of the growth, and pull it up the needle with a syringe of sterilized water.
-Inject the collected sample into a new plate and allow it to grow.
-Repeat if needed for best results.
-Make a plate using a mason jar with a Whatman and a SHIP on it.
-Grow that plate out then turn it into a LC by injecting sterilized water in it to break up the growth and suspend it in the water.
-Make a bunch of LC syringes to then take to grain, more agar, etc.


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Never Judge a man until you walk a mile in his shoes, because then you are a mile away and you have his shoes.


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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: MadHatR]
    #26895908 - 08/23/20 07:43 PM (3 years, 5 months ago)

Yes I've done pretty much all that. You're better off removing the lid than using a ship. I prefer nothing makes physical contact with my syringe. It sounds like you're not confident in your SAB skills?

Making transfers is much safer with a sterile blade I would never use a water syringe to transfer plates.


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OfflineMadHatR
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #26896209 - 08/24/20 12:07 AM (3 years, 5 months ago)

Quote:

Munchauzen said:
Yes I've done pretty much all that. You're better off removing the lid than using a ship. I prefer nothing makes physical contact with my syringe. It sounds like you're not confident in your SAB skills?




Actually, if anything I normally overdue it. 17 years ago when I was I heavily practicing, my success rate was about 95%. After reading up over the last month I am surprised we were able to produce anything back then other than mold.

As for why I wanted to avoid unscrewing the lids. I like to remove as many possible variables when starting a new project. With fewer points of failure, if something goes wrong then it is easier to track it down. I also would like to for selfish reasons. Years of working in engineering has left me with a bit of a hand trimmer due to being exposed to fumes. Injecting the syringe is much easier than unscrewing, carefully moving the lid to the side, hovering the needle into the open container and spraying it onto the agar. Once I get around to building a flow-hood it will be a different story. 

Quote:

Making transfers is much safer with a sterile blade I would never use a water syringe to transfer plates.




I know the biopsy syringe idea is not a new one. Next to a swab, it is the most common way of transferring cultures from inside a organism to a dish to grow it out and study it. With a straight blade, like a scalpel, you have to slice up the agar like a pie and then use the same straight blade to lift up and carry out the slice. I know it is not that hard, but I can't see why using a blade in the shape of a cylinder, a needle, to punch a hole in the agar and then suck that sample into a clean suspended state isn't easier.

I am not disputing the facts, just looking to innovate, learn and maybe help others with special needs.


--------------------
Never Judge a man until you walk a mile in his shoes, because then you are a mile away and you have his shoes.


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: MadHatR]
    #26896225 - 08/24/20 12:34 AM (3 years, 5 months ago)

Its not that it's not easier its that it's more risky.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #26896226 - 08/24/20 12:34 AM (3 years, 5 months ago)

Like a lot more


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: MadHatR]
    #26896384 - 08/24/20 04:08 AM (3 years, 5 months ago)

Quote:

MadHatR said:
Quote:

Munchauzen said:
Yes I've done pretty much all that. You're better off removing the lid than using a ship. I prefer nothing makes physical contact with my syringe. It sounds like you're not confident in your SAB skills?




Actually, if anything I normally overdue it. 17 years ago when I was I heavily practicing, my success rate was about 95%. After reading up over the last month I am surprised we were able to produce anything back then other than mold.

As for why I wanted to avoid unscrewing the lids. I like to remove as many possible variables when starting a new project. With fewer points of failure, if something goes wrong then it is easier to track it down. I also would like to for selfish reasons. Years of working in engineering has left me with a bit of a hand trimmer due to being exposed to fumes. Injecting the syringe is much easier than unscrewing, carefully moving the lid to the side, hovering the needle into the open container and spraying it onto the agar. Once I get around to building a flow-hood it will be a different story. 

Quote:

Making transfers is much safer with a sterile blade I would never use a water syringe to transfer plates.




I know the biopsy syringe idea is not a new one. Next to a swab, it is the most common way of transferring cultures from inside a organism to a dish to grow it out and study it. With a straight blade, like a scalpel, you have to slice up the agar like a pie and then use the same straight blade to lift up and carry out the slice. I know it is not that hard, but I can't see why using a blade in the shape of a cylinder, a needle, to punch a hole in the agar and then suck that sample into a clean suspended state isn't easier.

I am not disputing the facts, just looking to innovate, learn and maybe help others with special needs.




Well if you have a disability or special needs, by all means do whatever you see fit for your circumstances. But I would find a method besides liquid biopsy syringes. Thats asking for trouble. Introducing water at every transfer is a pretty big vector. SHIPs are also a pretty big vector as well. maybe a tiny metal scoop of some kind to make transfers in a single movement? also, get some pop top lids instead of screw ons. they open with just a slight squeeze on the sides, maybe those would be easier to handle?


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OfflineMadHatR
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #26896391 - 08/24/20 04:15 AM (3 years, 5 months ago)

Quote:

tryptkaloids said:
Its not that it's not easier its that it's more risky.




How is it more risky? It's not that I doubt that you are correct, I just like to know the science behind it. Without knowing why something is happening deprives you improving and innovating. If we all just keep doing things a certain way for the soul reason that past experience has shown us that doing it that way works then we never advance. At one point we all thought you had to build a incubator to get grain to spawn. There was a time when Gloveboxes were considered a great option until someone pointed out that the negative air pressure caused by attaching gloves to the box actually pulled contaminated air into the box. If I didn't know the concept behind how a flow-hood works then I might think that taping a filter to a box fan and blowing it over your work area might help, sorry Willy.

I would think that by creating a sealed space inside the dish that the only thing that enters that space is a needle that was just flame sterilized would be a good thing. When you take the top off the dish then due to a change in air pressure air flow would be created in the SAB. The process of moving the lid out of the way to inject spores, I would think, would have a higher chance of contamination.

Sorry for the rant, but a few years ago I signed on, was reading up on all the Tek's and I floated the idea of taking half pint jars, pouring a layer of agar on the bottom, knock up, grow out, then inject water into the jar to break up mycelium that you could then suck back up to make LC syringes. I was told that if I wasn't confident enough to drop a wedge in a jar of LC solution then I shouldn't be working with agar. Fast forward a few years and I found out that not only was my idea not bad, but Pasty had already came up with a similar idea and was having good results with it.

I also don't understand why it is that we cut large wedges out for transfer instead of a needle biopsy. I know with a larger sample size, the mycelium should repair and start spreading faster than a small sample. This will give it a greater chance of fighting off any contamination, and of course move you toward the finish line faster. I have all the time in the world, and am in no rush because I know that I can time things so once you are finishing your last flush on your current batch your next batch is ready. The same concept that I used to use when growing green. In that case you would have 3 tents running all the time. Tent 1 would be in veg for 30 days, tent 2 would be on the first 30 days of bloom and the 3rd tent would be on the last 30 days of bloom. That allowed you to rotate the cycles around so that your were always harvesting.


--------------------
Never Judge a man until you walk a mile in his shoes, because then you are a mile away and you have his shoes.


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