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jbgtaa
extraterrestrial



Registered: 06/09/19
Posts: 1,785
Last seen: 3 years, 3 months
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Re: How well does a PC actually kill contaminates? [Re: Pastywhyte]
#26874542 - 08/11/20 07:24 AM (3 years, 5 months ago) |
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I can see two problems: not properly sterilizing with the instant pot, which isn't your fault, or there's a problem with the oats. Alot of people say oats are more likely to contaminate. Idk the truth behind it but i would switch to millet if I were you (or wbs). The instant pot probably works well enough, but you don't have as much control over temp or PSI, and it may not be reaching full sterilization. Coupled with dirty filters and you've got bad jars before noccing.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: How well does a PC actually kill contaminates? [Re: jbgtaa]
#26876279 - 08/12/20 03:47 AM (3 years, 5 months ago) |
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I have some, I use them for weed though mostly. I didn't think they'd be good for spawn jars since leak proof means no GE at all. I didn't wanna fuck up the nice black ones with SFDs.
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meowjinx
Stranger

Registered: 05/20/19
Posts: 492
Last seen: 1 year, 6 months
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Re: How well does a PC actually kill contaminates? [Re: Inocuole]
#26876310 - 08/12/20 04:51 AM (3 years, 5 months ago) |
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Quote:
Inocuole said: I have some, I use them for weed though mostly. I didn't think they'd be good for spawn jars since leak proof means no GE at all. I didn't wanna fuck up the nice black ones with SFDs.
Can't you just leave it loosened a little bit? Seems like even w/ plastic thing there'd still be room for adequate GE if you loosen it a bit more than the regular ones
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Neowynd8

Registered: 06/12/16
Posts: 277
Last seen: 7 months, 1 day
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Re: How well does a PC actually kill contaminates? [Re: mushman1017]
#26877137 - 08/12/20 04:28 PM (3 years, 5 months ago) |
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If contaminates are intoduced after sterilization, they could be dispersed by shaking the jar or more likely dissolved in condensation running down the side of the jar.
Probably lids if you had no problems before...try to determine the psi the pot operates at
Edited by Neowynd8 (08/12/20 04:32 PM)
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nooneman


Registered: 04/24/09
Posts: 14,555
Loc: Utah
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Re: How well does a PC actually kill contaminates? [Re: mushman1017]
#26877182 - 08/12/20 04:55 PM (3 years, 5 months ago) |
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Try washing your jars. You say you've been using the same ones since the beginning, and recently you've had trouble even with longer times. Not saying washing will necessarily help, but it's definitely something to try.
PCing doesn't necessarily kill everything, just enough to give the mycelium a fighting chance at colonizing before the other stuff gets to it.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: How well does a PC actually kill contaminates? [Re: meowjinx]
#26877366 - 08/12/20 06:59 PM (3 years, 5 months ago) |
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Quote:
meowjinx said:
Quote:
Inocuole said: I have some, I use them for weed though mostly. I didn't think they'd be good for spawn jars since leak proof means no GE at all. I didn't wanna fuck up the nice black ones with SFDs.
Can't you just leave it loosened a little bit? Seems like even w/ plastic thing there'd still be room for adequate GE if you loosen it a bit more than the regular ones
If I'm gonna use an airtight lid just to loosen it, why not just use the non-airtight lid tho?
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mushman1017
Stranger
Registered: 08/27/19
Posts: 168
Last seen: 2 years, 5 months
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Re: How well does a PC actually kill contaminates? [Re: nooneman]
#26885924 - 08/18/20 12:35 AM (3 years, 5 months ago) |
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Quote:
nooneman said: Try washing your jars. You say you've been using the same ones since the beginning, and recently you've had trouble even with longer times. Not saying washing will necessarily help, but it's definitely something to try.
PCing doesn't necessarily kill everything, just enough to give the mycelium a fighting chance at colonizing before the other stuff gets to it.
Ya Ive already tried replacing the tyvek filters and i still get contam in it... only thing left to try is making new jars completely in case there are any contams left on the under side of the lid, even after washing it away. Other than that, Ive got no clue what else it could be... my prep work is the same that Ive been doing from the beginning.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: How well does a PC actually kill contaminates? [Re: mushman1017]
#26886217 - 08/18/20 07:36 AM (3 years, 5 months ago) |
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Quote:
mushman1017 said:
Quote:
nooneman said: Try washing your jars. You say you've been using the same ones since the beginning, and recently you've had trouble even with longer times. Not saying washing will necessarily help, but it's definitely something to try.
PCing doesn't necessarily kill everything, just enough to give the mycelium a fighting chance at colonizing before the other stuff gets to it.
Ya Ive already tried replacing the tyvek filters and i still get contam in it... only thing left to try is making new jars completely in case there are any contams left on the under side of the lid, even after washing it away. Other than that, Ive got no clue what else it could be... my prep work is the same that Ive been doing from the beginning.
Tyvek is generally considered to be only good for one run. I wouldn’t reuse a tyvek filter.
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