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Offlinemushman1017
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How well does a PC actually kill contaminates?
    #26873245 - 08/10/20 12:38 PM (3 years, 5 months ago)

So ive been doing my thing for a little while... and at first had pretty good success... Ive been trying to reuse things as much as possible to keep costs down,going green by recycling materials, etc... but im starting to wonder now that Ive had some consistent contam issues, where my flaws could be coming from.

It seems now that Ive been getting about a 50% clean spawn ratio over the past month or two.  At first I thought it was because I was doing G2G and was transferring dirty spawn (which is def a possibility).  However, I also noticed that some of my jars already had mold in it prior to me inoculating (after I PC and store in a clean tub with lid on). Ive been using oats for a while now... producers pride. Normally in the past Ive PC'd for about 2 hours... but after these contam issues Ive upped it to 2.5 hours with not much better results.

I have been using metal lids with tyvek material as the breathing filter... however Ive pretty much been reusing these strait from birthing. After its birthed I rinse out the jars, but the filters just have been thrown back in before PC'ing the new upcoming jars. I havent been worried about cleaning or using new ones cause I thought the PC cycle kills pretty much anything in there... Is it possible the tyvek is just getting old, and maybe clogged up, which is preventing proper air exchange to some jars?

I dont think its my technique cause ive been doing the same things I have been from day one... never put anything above an open jar or container, spraying near the openings of containers with iso prior to doing any transfers, etc...  Thankfully oats are cheap but this is getting beyond frustrating.


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OfflineRapjack
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Re: How well does a PC actually kill contaminates? [Re: mushman1017]
    #26873266 - 08/10/20 12:47 PM (3 years, 5 months ago)

I'd try swapping to fresh filters and see if that changes things. I don't think they clog but rather develop tiny holes eventually. If you use a large disc of tyvek it might also be warped and not sitting flush anymore.

To answer the question: Very very well actually. 90+ mins at 15 PSI sterilizes to log 6 which means one in a million microorganisms survive.


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Edited by Rapjack (08/10/20 12:49 PM)


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OfflineTight Lunchbox
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Re: How well does a PC actually kill contaminates? [Re: mushman1017]
    #26873272 - 08/10/20 12:49 PM (3 years, 5 months ago)

I never felt comfortable using the same tyvek filter more than twice. I also used two layers.

Try using new filters the next time you pc some jars and see if there is a difference.


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Edited by Tight Lunchbox (08/10/20 01:46 PM)


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Offlinemushman1017
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Re: How well does a PC actually kill contaminates? [Re: Tight Lunchbox]
    #26873374 - 08/10/20 01:39 PM (3 years, 5 months ago)

Ok cool ill give it a try and toss all the old tyvek discs...  Pretty sure Ive reused these tyvek discs at least 10-30 times by now each lol.


Edited by mushman1017 (08/10/20 01:40 PM)


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OfflineTight Lunchbox
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Re: How well does a PC actually kill contaminates? [Re: mushman1017]
    #26873392 - 08/10/20 01:46 PM (3 years, 5 months ago)

Haha, yeah a tyvek paint suit is like 9 bucks and you can get a shit ton of discs from it, so it's well worth it imo.


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InvisibleGanja420Boy
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Re: How well does a PC actually kill contaminates? [Re: Tight Lunchbox]
    #26873410 - 08/10/20 01:51 PM (3 years, 5 months ago)

How long are the jars being PC'ed at "?",you didn't mentionable that.


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OfflineTight Lunchbox
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Re: How well does a PC actually kill contaminates? [Re: Ganja420Boy]
    #26873451 - 08/10/20 02:09 PM (3 years, 5 months ago)

2-2.5 hours is what he says in the op


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InvisibleGanja420Boy
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Re: How well does a PC actually kill contaminates? [Re: Tight Lunchbox]
    #26873465 - 08/10/20 02:20 PM (3 years, 5 months ago)

Oh my bad, had quite a bit to drink and looked over what I typed. I actually meant to ask about the jar size?


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InvisiblePastywhyteMDiscord
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Re: How well does a PC actually kill contaminates? [Re: Ganja420Boy]
    #26873466 - 08/10/20 02:22 PM (3 years, 5 months ago)

I don’t use filters on my grain spawn lids anymore.


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OfflineTstone
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Re: How well does a PC actually kill contaminates? [Re: Tight Lunchbox]
    #26873468 - 08/10/20 02:22 PM (3 years, 5 months ago)

All I can say is, I'm a poly man. Surpassed 1500 jars, not a single tam.
Majority of people on here with most reliable outcomes with jars is, old fashioned poly. Just saying... Now, could also be your G2G method, but even though it might be a little inconvenience, I'm a proponent of poly for lids. Just me though.. don't think any tyvek folks can say that. And I get about 120 jars out of a bag of poly, can't get more economical than that.

No tyvek will last 30 PC rounds. Not much other than glass gonna last 30 PC rounds.



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Edited by Tstone (08/10/20 02:36 PM)


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OfflineNeowynd8
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Re: How well does a PC actually kill contaminates? [Re: mushman1017]
    #26873480 - 08/10/20 02:28 PM (3 years, 5 months ago)

Mold before inoculation and after PCing? Huge problem...are you venting PC prior to cycle? Does your PC have a gauge?


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Offlinemushman1017
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Re: How well does a PC actually kill contaminates? [Re: Neowynd8]
    #26873834 - 08/10/20 06:22 PM (3 years, 5 months ago)

Quote:

Neowynd8 said:
Mold before inoculation and after PCing? Huge problem...are you venting PC prior to cycle? Does your PC have a gauge?





Im using the 8 quart instant pots for my PC... I do it in all sorts of fashions... sometimes after I run it for 2 hours (2.5 lately), soon as I hear its done ill go to the PC and push the release valve so it can release pressure and cool down quicker. Other times Ive let it sit for 24 hours and gotten it the next day. If I do let it sit longer Ive noticed the aluminum foil tends to hold some of the moisture... and the tops of the tyvek are a bit more wet than Id normally have liked. However Ive done all these methods from the time Ive started, and its only been over the past month or two Ive been having trouble with contam.

At this point only thing I can think of is there are small holes made in the tyvek from Pc'ing over and over again, which in turn is letting stuff get in, even while sitting in my "clean box" waiting to be noc'd up.  In fact thats kind of been the only way I can see if its worth noc'ing up at this point, is do my PC for my jars, let it sit for a week and see if anything grows inside or not. Hoping its the tyvek. Post office envelopes has been working up to this point, and its a much smoother material than the tyvek suits (started with those but its hard to get them to keep form properly). And fyi I dont really care if  someone says I shouldnt be taking shit from the post office. lol.


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Offlinemushman1017
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Re: How well does a PC actually kill contaminates? [Re: Pastywhyte]
    #26873835 - 08/10/20 06:23 PM (3 years, 5 months ago)

Quote:

Pastywhyte said:
I don’t use filters on my grain spawn lids anymore.




If you dont use filters how do you get it to breath properly with GE and what not?


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Offlinesendmehummus
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Re: How well does a PC actually kill contaminates? [Re: Tstone]
    #26873843 - 08/10/20 06:27 PM (3 years, 5 months ago)

Not to mention an absurdly large bag of poly is like.. 3$.. Im poly for life I love it.


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OfflineGan
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Re: How well does a PC actually kill contaminates? [Re: mushman1017]
    #26873849 - 08/10/20 06:29 PM (3 years, 5 months ago)

Quote:

mushman1017 said:
Quote:

Pastywhyte said:
I don’t use filters on my grain spawn lids anymore.




If you dont use filters how do you get it to breath properly with GE and what not?




Loosen the lid a tad


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Offlinemushman1017
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Re: How well does a PC actually kill contaminates? [Re: Gan]
    #26874267 - 08/10/20 11:15 PM (3 years, 5 months ago)

Ok so just for reference, ive pc'd and let these jars sit for about a week while Ive had other shit going on... and was waiting for my LC to be ready to noc these jars usp.

4 out of the 24 were bad... 3 of them looked like this :


and one looked like this :



Notice its growing not just at the top but in the middle too... Its still possible to spread from the top down in just partial spots if the tyvek was bad? Wouldnt there have to be spores all over for it to be this rampant within a week? I dont get it...


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Offlinemeowjinx
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Re: How well does a PC actually kill contaminates? [Re: mushman1017]
    #26874280 - 08/10/20 11:40 PM (3 years, 5 months ago)

My money would be on the filter. Sterilizing grains kills the contams that existed in the grain out of the bag/after boiling, but once it comes out of the PC those sterile grains are free for the taking for any microbe that finds its way into the jar.

You could have perfect sterile technique in your SAB when inoculating your sterile jars, but once you move them out of the SAB into wherever it is you keep them during your colonization stage they'd be left quite vulnerable
if you have bad filters

I doubt I'd ever use anything other than SFDs when using a filter material for jar lids,but I'm gonna be using Pasty's unmodded plastic lids for my current grow. The peace of mind of not worrying about microscopic tears or improper adhesion of the filter would be so great


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Offlinetrippleblack
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Re: How well does a PC actually kill contaminates? [Re: meowjinx]
    #26874291 - 08/10/20 11:56 PM (3 years, 5 months ago)

my filters looked moldy, some of my jars are cruddy -from pressuring cooking bad spawn jars then dumping the crud out.  i'm pretty sure the pressure cooker kills everything, so your contam is likely not coming from your cruddy jars.  i got some jars 6 years old, sealing rings and jar lids that crumble like ash if i press to hard. that never cause contam if the barrier was kept.


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InvisibleInocuole
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Re: How well does a PC actually kill contaminates? [Re: Pastywhyte]
    #26874312 - 08/11/20 12:22 AM (3 years, 5 months ago)

Quote:

Pastywhyte said:
I don’t use filters on my grain spawn lids anymore.



What lids you using these days bro?  White plastic?


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InvisiblePastywhyteMDiscord
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Re: How well does a PC actually kill contaminates? [Re: Inocuole]
    #26874518 - 08/11/20 07:02 AM (3 years, 5 months ago)

Quote:

Inocuole said:
Quote:

Pastywhyte said:
I don’t use filters on my grain spawn lids anymore.



What lids you using these days bro?  White plastic?




Mostly but I did just pick up a few dozen black leak proof ones, gonna see what the buzz is about.


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