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OfflineUnknown88
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Registered: 08/10/20
Posts: 2
Last seen: 3 years, 5 months
MS on agar, contamination or mycelium?
    #26873134 - 08/10/20 11:40 AM (3 years, 5 months ago)

I have been reading these forums for months now, I'm still a beginner and want to be more involved with the community, I have had success with cultivation and love learning about this hobby, one of my issues is thst I stress quite a bit about things and would like to know if some of my MS agar plates are contaminated or not and would like an explanation to why that is, I'm interested in the understanding of the diagnosis. Thankyou to anybody that gives me their time and knowledge. Also yes, I have seen other pictures and references and have read about contaminations but I'm a little unsure about this and would like guidance, thanks. 😊



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OfflineEntheoGod
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Registered: 03/06/15
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Re: MS on agar, contamination or mycelium? [Re: Unknown88]
    #26873260 - 08/10/20 12:45 PM (3 years, 5 months ago)

From my understanding this is what a germ plate looks like, take a healthy white piece and transfer that to a new clean agar plate. That will tell you for sure. It looks like a normal germ plate to me.

Some of those have bacteria in them though. Choose the cleanest one and take a nice white section to a new plate.

The 5th and 2nd pic are bacterial.


Edited by EntheoGod (08/10/20 12:48 PM)


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OfflineBroken Veils
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Registered: 04/26/20
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Last seen: 3 years, 26 days
Re: MS on agar, contamination or mycelium? [Re: EntheoGod]
    #26873869 - 08/10/20 06:45 PM (3 years, 5 months ago)

They are all slightly or seriously bacterial...not to worry you can solve with some transfers...plate 3 is my favorite candidate...try to get a grain of rice sized transfer from a leading edge clean growth...repeat 2-3 times and go from there!


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InvisibleRoger Clemency
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Registered: 03/23/20
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Re: MS on agar, contamination or mycelium? [Re: Broken Veils]
    #26873981 - 08/10/20 07:48 PM (3 years, 5 months ago)

Yup, cut out a little piece of white with non bacterial agar around it (the milky or thick cloudy areas) and transfer to a new plate.

When you germinate spores you should try to keep them either in a single spot, or streak them in a line or Z pattern. That way you don’t have chaotic plates and you will know where you put spores so anything popping up elsewhere is a contaminant, and you’ll also have clear areas along the streak for the myc to grow into and it’ll be easier to tell what’s up and select good growth.

Welcome to the Shroomery:mushroom2:

day/month/year?? Blasphemy! It’s been month/day/year since Socrates wore short pants.


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Sour grapes, sweet revenge
Heaven starts right where hell ends


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OfflineUnknown88
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Registered: 08/10/20
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Last seen: 3 years, 5 months
Re: MS on agar, contamination or mycelium? [Re: Roger Clemency]
    #26874164 - 08/10/20 09:38 PM (3 years, 5 months ago)

Excellent, thanks everybody for the clarification 😊


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OfflineGan
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Registered: 08/26/19
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Last seen: 5 months, 27 days
Re: MS on agar, contamination or mycelium? [Re: Unknown88]
    #26874182 - 08/10/20 09:54 PM (3 years, 5 months ago)

Also, transfer early from germination plates if possible. Dont let them get grown out


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