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OfflineNobodycare
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Bacterial or rhizo?
    #26873111 - 08/10/20 11:25 AM (3 years, 5 months ago)

I just cant ever tell. Is this strand like growth signs or bacterial conditions or just a little rhizo growth? I will def spawn this jar and not be concerned but I was hoping to use for G2G. Should i ditch that idea and just spawn it or would you guys still do the G2G?



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Offlinegabbk
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Re: Bacterial or rhizo? [Re: Nobodycare]
    #26873129 - 08/10/20 11:35 AM (3 years, 5 months ago)

Looks bacterial to me. Looks it's just starting to spread out.
It looks to me the type of bacteria that comes from the inoculum (agar, syringe or LC)


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Edited by gabbk (08/10/20 11:36 AM)


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OfflineNobodycare
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Re: Bacterial or rhizo? [Re: gabbk]
    #26873153 - 08/10/20 11:51 AM (3 years, 5 months ago)

So im guessing I should spawn vs g2g?


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OfflineA.k.aM
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Re: Bacterial or rhizo? [Re: Nobodycare]
    #26873170 - 08/10/20 11:59 AM (3 years, 5 months ago)

Yeah I’d for sure spawn it but not g2g.


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OfflineEntheoGod
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Re: Bacterial or rhizo? [Re: A.k.a]
    #26873226 - 08/10/20 12:29 PM (3 years, 5 months ago)

Try spawning it into a smaller size tray rather than a full mono tub.

Bacterial jars still fruit but I’ve noticed the seeds won’t colonize substrate as quick and it will sometimes just stop colonizing.  The less substrate there to colonize, the more likely it will fully colonize the sub. Here’s a few bacterial trays I made...



Give it a shot! Good luck.


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Offlinegabbk
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Re: Bacterial or rhizo? [Re: EntheoGod]
    #26873573 - 08/10/20 03:30 PM (3 years, 5 months ago)

Yup! Bacterial spawns definitely can be spawned!

The last few months I've been dealing with the same kind of bacteria and reached to do a comparison using H2O2 (hydrogen peroxide). I did two tubs with SFF shoebox Tek (by ShaperDreaming) but instead of spraying down both casing with regular water I did one with 3% hydrogen peroxide water and the other one with tap filtered water.

Here it is a pic of both of them at day six from birth (the one above is the casing with hydrogen peroxide, and the one below with regular water)



And here the final results:


The one at the right is the casing with H2O2. As you can see the it had way better result than with regular water IN THIS CASE. Lots of people will bash the use of hydrogen peroxide. But I personally think it can have it's place, JUST when you encounter this type of bacteria in the jar (carried within the initial inoculum) and the best bet (or last resort) is to use it instead of throwing these jars away or get that shitty delayed growth as the tub at the left if you use regular water.

Worth mentioning that these jars took 6 weeks to fully colonize and took a shitton of effort to break the grains apart (both clear signs of bacterial infestation).
Hydrogen peroxide has antibacterial properties, so it makes sense that the tub I didn't spray it with had very shitty results, manifested in delayed growth and not even reaching fully mature bodies.

Don't get me wrong; just to be clear, by no means I'm advising the use hydrogen peroxide as regular practice. I finally realised it was my poor sterile technique the issue and didn't have to use it anymore because I now inoculate just clean agar (at that time I couldn't recognize it. You can check one of my posts 'wrinkles' on agar? to see what I'm taking about; my agars had always a leading halo underneath the mycelium that didn't even seem to be contamination if you looked quickly).

But anyways. To sum up: you should have clear inoculum and spawn from the get go, and that way not having to use H2O2 in any stage of cultivation in spite it can be useful in these cases.

I hope you can give it a try and let me know how it goes!


Edited by gabbk (08/10/20 03:36 PM)


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Invisiblemaxmush
Always learning...
Registered: 06/13/20
Posts: 440
Re: Bacterial or rhizo? [Re: gabbk]
    #26873821 - 08/10/20 06:16 PM (3 years, 5 months ago)

Watch out for the lasers eyes coming at ya with the h202 lol


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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