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Offlinedjdc
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Registered: 06/11/20
Posts: 84
Last seen: 3 years, 4 months
first time agar
    #26872493 - 08/09/20 10:38 PM (3 years, 5 months ago)

I am going for my first shot at agar, bought some prelaid agar plates. I did one clone, 2 from spore prints, and 6 from spore syringes. What kind of timeframe am I looking at to see growth?


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Offlineskullhuman
the skullman cometh
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Registered: 06/15/09
Posts: 1,473
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Re: first time agar [Re: djdc]
    #26872495 - 08/09/20 10:40 PM (3 years, 5 months ago)

You'll see the clone start making headway in a couple of days.  Depending on the viability of spores, you should expect growth anywhere from 4 days to a few weeks to never.  Prints work better on agar than syringes but is what is- if the spores are any good you'll see mycellium either way.


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Offlinescarabaeus
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Registered: 06/13/18
Posts: 1,101
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Re: first time agar [Re: djdc]
    #26872531 - 08/09/20 11:42 PM (3 years, 5 months ago)

I agree with Skull ^ above. Also, hydrated spores will germinate faster than old dry spores (until they won't germinate at all). A fresh spore print is fast, as in a few days, an old spore print can take a month or more IME.

Spores in a spore syringe are of course already hydrated, but some people don't have the same success as they do with prints. Maybe because of the vendor they purchased them from? IDK. Personally I have had pretty much equal success with both spore streaks on agar from prints, and spore drops on agar from spore syringes. But that is just me.


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Offlinedjdc
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Registered: 06/11/20
Posts: 84
Last seen: 3 years, 4 months
Re: first time agar [Re: scarabaeus]
    #26873388 - 08/10/20 01:44 PM (3 years, 5 months ago)

Thank you for the responses. The dry spores are about a month old, the syringes I've had success with into grain so hoping for the best.

I've just read that scoring the agar helps with multi spore is this true? I didn't do that with any of them... If so can I open them and do that now?


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