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rschloz
Naked Stranger
Registered: 08/03/20
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What am I forgetting?
#26872369 - 08/09/20 09:20 PM (3 years, 5 months ago) |
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So I have been reading and reading, and then doing some more reading, and then reading some more. I think I've finally decided I'm ready to embark on the cultivation journey, and this post represents my humble synthesis of all the knowledge I think I have gleaned in my studies. I wrote my own instruction manual using several different teks, and I'd like to thank the authors for making their knowledge available to us. I would like to know if I'm on the right track before I begin, and if I'm forgetting anything critical.
One note, I only have an Instant Pot as a pressure cooker, so I'm going to roll the dice using that instead of a larger manual model.
If this looks ok, I'll try to document my experience as I go, and provide a writeup with results. This is in rough chronological order. Thanks in advance for reading the wall of text.
1. SAB a. 70-100qt bin b. Knife
Cut 6” holes in side of bin, smooth with sandpaper if needed.
2. Prep pasty plates a. 1 tsp agar agar b. 2 tsp potato flakes c. Drop of honey d. ½ c water e. Drop of food coloring f. 8 glad rounds i. Prep with hole in each and mp tape over hole g. Paper towel h. foil
Combine first five ingredients over low heat until fully dissolved. Pour into glad rounds. Fold paper towel into square and place on lid of each glad round. Cover with tin foil squares. PC for 1 hr on high (Instant Pot).
3. Inoculate pasty plates a. Spore syringe b. 4 prepped plates c. SAB d. Lighter or torch
Put all 4 prepped plates into SAB. Shake syringe, flame SS needle outside of SAB, and place one drop onto prepped plate. Repeat shake and flame for remaining three plates. Put 4 plates into Ziploc and hold at room temp to wait for growth (est. 7-10 days). Store 4 non-inoculated plates in separate Ziploc.
4. Clean up plates a. Inoculated plates b. Scalpel c. SAB d. Lighter or torch
In SAB, assess plates for mycelial and bacterial growth. Find the best mycelium available and use flamed scalpel to remove rice-sized portion and transfer to unused plates. Wait another 7-10 days to assess growth. Repeat transfer if necessary (repeat agar prep). Once clean healthy growth has been established, move on to next step.
5. Prep grains (hamloaf) a. 1000g of rye berries (250g per quart jar) b. Large pot c. 4 quart sized mason jars d. Foil
Put rye into pot and rinse with warm water. Repeat. Fill pot with water 3 inches above grains and put onto high heat until rolling boil. Boil 10 minutes and turn off heat. Let grains soak for additional 10 minutes, and then strain into strainer. Occasionally stir or shake grains until steam stops escaping or grains return to room temperature. Fill grain into jars 2/3 to ¾ full and cap jars. Cover jars in tin foil, lightly unscrew jars a quarter turn, and PC 2 hours on high (Instant Pot).
6. Inoculate grains a. Clean pasty plate b. 4 jars of PC’d grains c. SAB d. Lighter or torch
Spray SAB sides lightly with soap water and let sit to trap airborne particles. Place PC’d grain jars and clean plate into box. Unscrew bands of jars, but don’t pop tops until transferring. Working carefully not to put non-sterile hands above sterile surface, slice a piece of clean mycelium with flamed scalpel, and drop into each grain jar. Screw on jar lids finger tight, and then unscrew quarter turn for gas exchange. Put jars away for 7 days and then assess colonization. At approximately 25-30% colonization shake jars vigorously to redistribute mycelium. After another 3-7 days jar should be at 100% colonization and ready to spawn to bulk substrate.
7. Making bulk substrate a. Brick of coco coir b. Water c. Bucket or cooler
Weigh coir in grams, and then multiply gram weight by 5 to get weight of water. Divide weight of water by 1000 to get liters needed. Boil water and then dump on brick of coir. After approx.. 90 minutes stir coir up and let sit overnight to cool. Test coir for field capacity (few drips to small stream when squeezing the shit out of it).
8. Spawning to bulk a. 4 -8 qt. hydrated coir substrate b. 4 fully colonized quart jars c. Unmodified 54qt sterilite tub
Vigorously shake all 4 jars and dump into bin. Add 4-8 quarts of substrate and mix the hell out of everything. Level out substrate but do not pack down. Lightly tamp down edges and corners of bin to help prevent side pinning. Flip lid and wait. Substrate should colonize for 8-10 days before pins show up. Substrate surface level should have consistent beads of moisture on surface level. Mist only on as-needed basis if surface begins to look dry.
9. Harvest
Once majority of pins have grown and veils are starting to break it is time to harvest. For easiest harvest fill the tub up with water until substrate floats to the top, and use a sharp knife to cut off mushrooms at the base. Dehydrate overnight immediately. Let substrate sit for 1-2 hours in water and then siphon or carefully pour water out of tub to prepare for second flush.
10. Go camping and enjoy fruits of labor
-------------------- Those who dance are considered insane by those who cannot hear the music.
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: What am I forgetting? [Re: rschloz]
#26872403 - 08/09/20 09:46 PM (3 years, 5 months ago) |
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yeah, sounds good to me. I can't see anything blatently worrying
-------------------- Life’s shit, but I’m loving it
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: What am I forgetting? [Re: Wall.E]
#26872409 - 08/09/20 09:49 PM (3 years, 5 months ago) |
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Well now that I re-read it, you're probably gonna wanna do something different with your bulk spawn. I don't think pouring boiling water on it and leaving it sit overnight is enough to pasteurize the coir.
However, I'm also super paranoid with my coir and pc it for 30 minutes. Don't go off my experiments though, I only have two subpar grows under my belt.
-------------------- Life’s shit, but I’m loving it
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: What am I forgetting? [Re: Wall.E]
#26872411 - 08/09/20 09:50 PM (3 years, 5 months ago) |
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you forrgot lots of weed.
stick with a torch. lighters are obnoxious when doing culture work.
looks like you payed attention to important parts.

next step is actually doing it.
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Gan
Wielder of Narya



Registered: 08/26/19
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Re: What am I forgetting? [Re: rschloz]
#26872416 - 08/09/20 09:52 PM (3 years, 5 months ago) |
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1.) Heat your knife or tub if you're going to go that route. If not, it can crack the plastic if you're sawing on it with a knife. I prefer using a hot as shit metal coffee container.
2.) Measure your agar ingredients by weight if you can to produce more consistent and repeatable results. Ex. 1000 mL (1 L) water, 20 g agar powder, 15-20 g LME.
3.) Use a spore print instead of a syringe if possible. Easier to work with on agar. Not a big deal if you already bought a syringe though. Include an inoculation loop to your list. Either buy some or make them yourself really easy. Streak your spores/spore solution in a zig zag across the plate at the least.
Good luck
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AlsetAlokin
Student

Registered: 07/30/20
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Re: What am I forgetting? [Re: Gan]
#26872447 - 08/09/20 10:10 PM (3 years, 5 months ago) |
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lids. https://www.shroomery.org/forums/showflat.php/Number/20048747#20048747
Cracking the grain jars probably won't suffice and could lead to contamination.
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AlsetAlokin
Student

Registered: 07/30/20
Posts: 182
Last seen: 3 years, 19 days
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I'm also sure there's much better resources in the search engine for PC times using an instantpot, but they don't truly remain at 15psi. Cook times may need to be adjusted.
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mushboy
modboy



Registered: 04/24/05
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Quote:
AlsetAlokin said: Cracking the grain jars probably won't suffice and could lead to contamination.
damn near all of us inoculate by opening the jar lids.
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AlsetAlokin
Student

Registered: 07/30/20
Posts: 182
Last seen: 3 years, 19 days
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Re: What am I forgetting? [Re: mushboy]
#26872467 - 08/09/20 10:24 PM (3 years, 5 months ago) |
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Quote:
mushboy said:
Quote:
AlsetAlokin said: Cracking the grain jars probably won't suffice and could lead to contamination.
damn near all of us inoculate by opening the jar lids.
Below 6 it looks like he's using unmodified metal lids for grain spawn, is this a thing? Everything I've read says some sort of filter or modified lid for GE.
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mushboy
modboy



Registered: 04/24/05
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oh snaps.
good catch. unless its one piece metal lids??
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rschloz
Naked Stranger
Registered: 08/03/20
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Last seen: 9 months, 20 days
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Re: What am I forgetting? [Re: mushboy]
#26872485 - 08/09/20 10:34 PM (3 years, 5 months ago) |
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They are 2 piece. I’ll do some reading on how to modify.
-------------------- Those who dance are considered insane by those who cannot hear the music.
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mushboy
modboy



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Posts: 32,274
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Re: What am I forgetting? [Re: rschloz]
#26872490 - 08/09/20 10:37 PM (3 years, 5 months ago) |
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rschloz
Naked Stranger
Registered: 08/03/20
Posts: 9
Last seen: 9 months, 20 days
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Re: What am I forgetting? [Re: mushboy]
#26898506 - 08/25/20 10:52 AM (3 years, 5 months ago) |
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So it's now been 11 days since I inoculated two pasty plates with spores, and I'm seeing 0 growth (no contams no myc). I suspect at this point my issue is the gel food coloring I used, which is full of preservatives and other unwanted ingredients.
I'm going to noc up two new plates today or tomorrow with no food coloring and see if I can't get something growing.
No pics because there's nothing to show so far. Just a green ass plate of jello. Onward for science!
-------------------- Those who dance are considered insane by those who cannot hear the music.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
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Re: What am I forgetting? [Re: rschloz]
#26898561 - 08/25/20 11:31 AM (3 years, 5 months ago) |
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Something spores can take a bit. I've used gel dye its fine.
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rschloz
Naked Stranger
Registered: 08/03/20
Posts: 9
Last seen: 9 months, 20 days
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Re: What am I forgetting? [Re: mushboy]
#26898604 - 08/25/20 11:58 AM (3 years, 5 months ago) |
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Ok that's good to hear.
So hard to have patience grrr. I haven't thrown anything away, just looking for more stuff to do while I wait.
-------------------- Those who dance are considered insane by those who cannot hear the music.
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rschloz
Naked Stranger
Registered: 08/03/20
Posts: 9
Last seen: 9 months, 20 days
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Re: What am I forgetting? [Re: rschloz]
#26904459 - 08/28/20 03:22 PM (3 years, 4 months ago) |
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We got some action!
-------------------- Those who dance are considered insane by those who cannot hear the music.
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