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maxmush
Always learning...
Registered: 06/13/20
Posts: 440
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Why do you use different agar recipies?
#26870677 - 08/08/20 09:04 PM (3 years, 5 months ago) |
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This may have been asked many times before, but i have not found any conclusive answers to this: why use different agar recipes?
1. is it to speed up growth 2. reduce contams 3. to provide different nuts for continued transfers (myc needs a change up of food) 4. for stubbornly germinating spores
Why not just use the standard plain agar or MEA? Please enlighten me
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SoupyGeorge
Hazy Daze since First Grade



Registered: 05/25/20
Posts: 216
Loc: Southern US of 'Merica
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Re: Why do you use different agar recipies? [Re: maxmush]
#26870686 - 08/08/20 09:13 PM (3 years, 5 months ago) |
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I'm just here for the info.
-------------------- Mellow heads will prevail. You look like everyone else because the T.V. tells you to. I look like everyone else so that i don't stand out to the man. We are not the same.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: Why do you use different agar recipies? [Re: SoupyGeorge]
#26870694 - 08/08/20 09:19 PM (3 years, 5 months ago) |
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ill change it if im bored. maybe the myc feels the same way? not sure.
antibiotic agars seem useful in some instances like cloning wild fruits. ive never used it tho.
if spores are stubborn i find a softer agar more useful than changing the nutes.
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FeedYourMind
indiGlo



Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 4 days, 7 hours
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Re: Why do you use different agar recipies? [Re: mushboy]
#26870713 - 08/08/20 09:52 PM (3 years, 5 months ago) |
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Quote:
mushboy said: ill change it if im bored. maybe the myc feels the same way? not sure.
Also, I have found the myc to stay more aggressive when keeping a constant rotation of nutes every transfer. I think you kind of hit on all of them. The faster the myc, the better the chance for it to consume the agar.
The other reason is grain water soak is incredibly cheap and can help cut into costs/inventory of buying supplies for MEA/PDA.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Why do you use different agar recipies? [Re: FeedYourMind]
#26870801 - 08/08/20 11:36 PM (3 years, 5 months ago) |
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Mostly #3 is why I would. I don't do it often. But adding a little bit of grain water to my potato flake agar seems to help prepare the mycelium to leap off onto grain better.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Why do you use different agar recipies? [Re: Inocuole]
#26870882 - 08/09/20 01:34 AM (3 years, 5 months ago) |
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I dont switch it up much at all. Myco standard 1.5% mea all day for me
For slants i use 3%
I use antibiotics sometimes to clean up a wild sample, or a rare sporeprint that is dirty af beyond easy cleaning w serial streaking. Sometimes i will use em on a plate before making slants, not usually tho. Very very rarely are they called for
Will play w the agar agar and nutrient % a bit if i have trouble germinting some tricky spores i really care about
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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