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Vylie
The more you know

Registered: 03/11/20
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Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* 1
#26869438 - 08/08/20 03:00 AM (3 years, 5 months ago) |
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Biscuits



Registered: 06/29/17
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26869453 - 08/08/20 03:39 AM (3 years, 5 months ago) |
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Question 2: Yes it does bruise a little in the fridge, but not much... Maybe if your fridge has higher temperature swings it would bruise more.
Sorry that you have to look through a sandwich bag, I didn't take that many glamour shots of fridge agar:
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Brian Jones
Club 27



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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26869461 - 08/08/20 03:49 AM (3 years, 5 months ago) |
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I'm not an expert but LC is a high reward, very high risk, proposition. Everyone's tried it but a lot of people don't use it anymore.
-------------------- "The Rolling Stones will break up over Brian Jones' dead body" John Lennon I don't want no commies in my car. No Christians either. The worst thing about corruption is that it works so well,
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* [Re: Brian Jones]
#26869501 - 08/08/20 05:05 AM (3 years, 5 months ago) |
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26869571 - 08/08/20 06:38 AM (3 years, 5 months ago) |
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Quote:
Vylie said: I’m not sure but it seems it worked when I used a fresh agar culture, and it didn't work when I used a culture from the fridge.
It seems like you have your answer right there. I always make 2 transfers away from my storage plate or slant. I don't like keeping my storage culture out long enough to start growing again so I take the first transfer and return to the fridge. After about 2-3 days I'll start getting growth, there is a bit of a lag on this first dish because it needs to "wake up" as you said. After it grows out to about the size of a quarter to make sure everything is looking good and symmetrical/clean, I make a second transfer and that second dish will be my production stock.
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26869645 - 08/08/20 08:06 AM (3 years, 5 months ago) |
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Quote:
Vylie said: In fact, PCed oat develops bacteria so fast that agar without LC is too slow for oat.
Where did you hear that?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Mushrooms’ adventures in the frige [Re: Vylie] 1
#26869684 - 08/08/20 08:33 AM (3 years, 5 months ago) |
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Quote:
Vylie said:
In fact, PCed oat develops bacteria so fast that agar without LC is too slow for oat.
Well that's the dumbest thing I've seen posted here all month.
100% unequivocally false.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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This how I do LC. If you want thick vigorous LC this is my preferred method to achieve it.
https://www.shroomery.org/forums/showflat.php/Number/23740857
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Mushrooms’ adventures in the frige [Re: bodhisatta] 1
#26869688 - 08/08/20 08:35 AM (3 years, 5 months ago) |
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Quote:
bodhisatta said:
Quote:
Vylie said:
In fact, PCed oat develops bacteria so fast that agar without LC is too slow for oat.
Well that's the dumbest thing I've seen posted here all month.
100% unequivocally false.
I’m suspecting a language barrier at play here.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: Mushrooms’ adventures in the frige [Re: Pastywhyte]
#26869697 - 08/08/20 08:42 AM (3 years, 5 months ago) |
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I hope so
I hate when people make up misinformation based on their experiences doing things wrong or poorly then they end up circulating as a "fact"
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
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Re: Mushrooms’ adventures in the frige [Re: bodhisatta]
#26869707 - 08/08/20 08:50 AM (3 years, 5 months ago) |
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Not sure how you could misinterpret that due to a language barrier. The implication seems to be that sterile oats develop bacteria before an agar wedge can colonize a jar. Not the case for me
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A.k.a
Stranger



Registered: 10/27/19
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It’s weird the cold myc doesn’t work, seems like it would be the same just a couple days slower.
Unless it can drown somehow before reactivating.
--------------------
LAGM2020     
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Mushrooms’ adventures in the frige [Re: A.k.a]
#26869806 - 08/08/20 09:59 AM (3 years, 5 months ago) |
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The problem is likely that the OP has poor technique or a dirty culture. It takes a bit for the mycelium to recover so if you have inadvertently contaminated the broth then the bacteria will take over before the mycelium has a chance to leap off, this would account for the lack of growth.
It's good practice to at least let the culture recover before using, even better to ensure that it's actually clean. I transfer twice just for redundancy measures, if you have a clean culture you can use it as soon as it recovers.
I've heard of gourmet farmers who won't use anything but fresh cultures because they claim a loss in vigor, the guy from earth angel makes this specific claim, I'm not sure how true that statement is but hey, who am I.
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* [Re: Pastywhyte]
#26869964 - 08/08/20 11:55 AM (3 years, 5 months ago) |
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Post deleted by Vylie
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Edited by Vylie (08/08/20 11:56 AM)
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* [Re: Vylie]
#26869978 - 08/08/20 12:06 PM (3 years, 5 months ago) |
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Post deleted by Vylie
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Edited by Vylie (08/08/20 12:07 PM)
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Josex
#cheat_code


Registered: 11/13/15
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Re: Mushrooms’ adventures in the frige [Re: Vylie] 1
#26869980 - 08/08/20 12:06 PM (3 years, 5 months ago) |
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Here for the popcorn
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* [Re: Josex]
#26869981 - 08/08/20 12:07 PM (3 years, 5 months ago) |
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26870082 - 08/08/20 01:09 PM (3 years, 5 months ago) |
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Entire point of pressure cooking. Killing endospores.
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26870099 - 08/08/20 01:18 PM (3 years, 5 months ago) |
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Quote:
Vylie said: By the way, if the bacteria develops always at the bottom of the jar while the top of it gets colonized, then it obviously does not come from my agar. It comes from the oat.
PC is PC. It’s not rocket science. If one did it a few times, it’s difficult to make it wrong.
So how do you explain the hundreds of cultivators using oats with no issues?
If you regularly get bacterial contamination at the bottom of your jars, I'd bet your grains are too wet.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
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Bet those oats are wet.
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Caps McGee
Grandaddy Smurfshack



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Re: Mushrooms’ adventures in the frige [Re: mushboy] 1
#26870102 - 08/08/20 01:20 PM (3 years, 5 months ago) |
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If you're getting bacteria at the bottom it IS the oats... but it's due to excess moisture: this may be a prep issue, or you might be using too much liquid innoculant... this is a stupid arguement: really ... try drying the oats better before loading them or not cooking them as long before pressure cycle, to cut back on your moisture level at the bottom... gotta minimize the conditions that breed bacteria: hot, wet, anaerobic conditions (like at the bottom of a jar/bag of grain with too much moisture present, either due to prep or over-inoculation) food for thought: if you're going to post a thread with questions, try not arguing with the people trying to help lol
Your title is unfinished: "the more you think you know, the less you can be taught"
 Wishing you the best
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Josex
#cheat_code


Registered: 11/13/15
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Re: Mushrooms’ adventures in the frige [Re: Caps McGee]
#26870107 - 08/08/20 01:24 PM (3 years, 5 months ago) |
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yeah oats need some serious drying to get them right IMO.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
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Re: Mushrooms’ adventures in the frige [Re: Josex]
#26870109 - 08/08/20 01:28 PM (3 years, 5 months ago) |
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Dude... I've been deying them completely on the outside, and if i don't undercook them also, the middle of the bags turn to mush... idk if its the 75X's not venting pressure vs presto or what... I'm having to relearn/redesign the whole prep process for bags... considering going back to jars so I can afford the shorter cycle Got to thinking also, I wonder if I'm bringing them to temp to quickly? Idk, but the steam definitely penetrates much deeper into the bags: monkey wrenches me pretty good, but we're looking better now woof
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Vylie
The more you know

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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Caps McGee]
#26870185 - 08/08/20 02:28 PM (3 years, 5 months ago) |
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Caps McGee]
#26870194 - 08/08/20 02:35 PM (3 years, 5 months ago) |
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Post deleted by Vylie
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Edited by Vylie (08/08/20 02:36 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26870199 - 08/08/20 02:39 PM (3 years, 5 months ago) |
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There's plenty of bacteria that can survive a normal pressure cooker cycle. But they're only found at the bottom of the ocean or other extreme places. The endospores used to validate autoclaves require a special incubator to grow just to check if they were killed or not
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26870208 - 08/08/20 02:43 PM (3 years, 5 months ago) |
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Quote:
Vylie said: If it’s coming because the oat is too wet, then it is coming from the oat. Period.
That is variable that is 100% under your control. Oat prep can be tricky, but once you get it down, it's great (and cheap!) for spawn. 👍
--------------------

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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Mushrooms’ adventures in the frige [Re: bodhisatta]
#26870209 - 08/08/20 02:44 PM (3 years, 5 months ago) |
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Quote:
bodhisatta said: The endospores used to validate autoclaves require a special incubator to grow just to check if they were killed or not
That's interesting, can you say more about that or maybe a link to an article on the subject?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26870212 - 08/08/20 02:47 PM (3 years, 5 months ago) |
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Quote:
Vylie said:
It will always be about a race between the mushrooms and the other organisms who either got inside or survived the PC.
They only get in if you let them in. Thats the point.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Mushrooms’ adventures in the frige [Re: Josex]
#26870232 - 08/08/20 03:02 PM (3 years, 5 months ago) |
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Quote:
Josex said:
Quote:
bodhisatta said: The endospores used to validate autoclaves require a special incubator to grow just to check if they were killed or not
That's interesting, can you say more about that or maybe a link to an article on the subject?
https://multimedia.3m.com/mws/media/497755O/attest-bi-for-steam-1262-package-insert-english.pdf
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
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Re: Mushrooms’ adventures in the frige [Re: bodhisatta]
#26870261 - 08/08/20 03:31 PM (3 years, 5 months ago) |
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Aren't those the strips I've seen you talk about on some occasions? Or these are a different thing? I wish someone would try sticking one of those things in a grain jar or bag. It'd be nice to know for sure, beyond anecdotal evidence or consensus, whether or not there are surviving endos after a cycle.
Edited by Josex (08/08/20 03:39 PM)
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
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Re: Mushrooms’ adventures in the frige [Re: Josex]
#26870357 - 08/08/20 05:06 PM (3 years, 5 months ago) |
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It's coming from the conditions created from improper prep: period... user error brotatoe
Sab isn't sterile: but your technique should be... SAB is a STILL air box, and operates on the principle of STILL air... hence slow concise thoughtful movement and hand placement, never putting more of your self or instruments over growth media than necessary
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* [Re: Caps McGee]
#26870974 - 08/09/20 04:41 AM (3 years, 5 months ago) |
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Vylie]
#26870982 - 08/09/20 04:57 AM (3 years, 5 months ago) |
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26871013 - 08/09/20 05:49 AM (3 years, 5 months ago) |
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We don't soak grain for endospores. You will end up with more than you started with.
The window of opportunity is a nearly 4 year old quote too. I don't really believe that shit at all anymore. if you don't inoculate a jar of sterilized oats it wont contaminate even after months of sitting if you did things right.
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Forrester
aspiring sociopath


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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26871017 - 08/09/20 05:52 AM (3 years, 5 months ago) |
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The idea that improper grain preparation can cause bacterial contamination is blatantly wrong. Having the grains too wet can cause mushy grain crap at the bottoms of jars, which may look like bacterial contamination, and may be prone to it because of it's soupy nature, but it does not cause it.
If you are getting consistent bacterial contamination in jars, either you are not sterilizing properly in the first place, or your technique is suffering and the bacteria is getting in somehow.
It's that simple.
edit: the "window of opportunity" idea can be helpful when referring to substrates spawned in open air, but that's about it.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
Edited by Forrester (08/09/20 05:53 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Mushrooms’ adventures in the frige [Re: Josex]
#26871020 - 08/09/20 05:57 AM (3 years, 5 months ago) |
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Quote:
Josex said: Aren't those the strips I've seen you talk about on some occasions? Or these are a different thing? I wish someone would try sticking one of those things in a grain jar or bag. It'd be nice to know for sure, beyond anecdotal evidence or consensus, whether or not there are surviving endos after a cycle.
Those are what I've mentioned. The problem is they're expensive and you have to have an account from a relevant business to even buy them.
For what its worth if your pressure cooker isn't broken in 15m at 15psi or 250F a biological indicator with 10^7 hard to kill endospores will come back completely dead. But that's not in a grain jar. I still suspect the 2 hours is more than overkill though.
Also, @OP If you search only to confirm your bias you won't learn shit.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26871048 - 08/09/20 06:26 AM (3 years, 5 months ago) |
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If agar wont colonize your lc I'd suspect 2 things.
Contamination of sorts or crap lc broth. Unless you are freezing your cultures the fridge is not the culprit.
Honey LCs gave me nothing but shit performance. I've seen people use em tho. 4% is also really high. Try 1-2% max.
Ps. You couldnt pay me to g2g that chunk of cheesey looking myc. That's gonna mold. You mine as well top fruit that chunk.
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Forrester]
#26871055 - 08/09/20 06:32 AM (3 years, 5 months ago) |
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Vylie]
#26871072 - 08/09/20 06:52 AM (3 years, 5 months ago) |
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Post deleted by Vylie
Reason for deletion: ...
Edited by Vylie (08/09/20 06:57 AM)
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Forrester
aspiring sociopath


Registered: 02/05/13
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26871079 - 08/09/20 07:01 AM (3 years, 5 months ago) |
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Glad it's working for ya. People just try to help, when you ask for help, so that you could do better. Not sure why you'd ask otherwise. One tends to learn more if they don't think they know everything already.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Mushrooms’ adventures in the frige [Re: Forrester]
#26871134 - 08/09/20 07:59 AM (3 years, 5 months ago) |
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Quote:
Forrester said: Glad it's working for ya. People just try to help, when you ask for help, so that you could do better. Not sure why you'd ask otherwise. One tends to learn more if they don't think they know everything already.
It feels so much better when you realize that you simply did something incorrectly that can be easily changed, even if the change is difficult it's still a relief.
"It is impossible to begin to learn that which one thinks one already knows."
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* *DELETED* [Re: Stipe-n Cap]
#26871189 - 08/09/20 08:47 AM (3 years, 5 months ago) |
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Post deleted by Vylie
Reason for deletion: ...
Edited by Vylie (08/09/20 08:51 AM)
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Vylie]
#26871193 - 08/09/20 08:51 AM (3 years, 5 months ago) |
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mushboy
modboy



Registered: 04/24/05
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26871216 - 08/09/20 09:06 AM (3 years, 5 months ago) |
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Try to keep things to one post please. Dont drag your own thread off topic by comparing people trying to help you to monkeys.
I dont think the oat jars are going to work. You could prep and dry them probably tho
No self delacred master gave you lc advice. From your link it looks like you listened to some random noob who said some noob shit but that's got nothing to do with your 'problem'. Your problem is user error.
Shit happens. Live and learn. Lighten up.
Edited by mushboy (08/09/20 09:09 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
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Re: Mushrooms’ adventures in the frige [Re: mushboy]
#26871229 - 08/09/20 09:16 AM (3 years, 5 months ago) |
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I not really worried about Bacillus thuringiensis itself surviving the PC cycle as much as I used to. Now I’m more concerned with it’s cry toxins that it produces. These cry toxins are pretty resistant to heat, more so than the bacteria that generates them. They also won’t do anything until exposed to swings in pH which activates them. There has now been some work on how they potentially can operate as a fungicide with promising results. It seems the metabolites exuded by at least some fungi, can activate the proteins and allow the toxin to inhibit fungi.
Nothing specific with cubes or other actives has been studied in this regard. But if Bt produced cry toxins are so far very effective against the limited species that have been looked at thus far, I don’t think it’s a stretch to hypothesize that they can’t be good for our cube grows.
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Vylie
The more you know

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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: mushboy]
#26871244 - 08/09/20 09:27 AM (3 years, 5 months ago) |
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Pastywhyte]
#26871280 - 08/09/20 09:46 AM (3 years, 5 months ago) |
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26871302 - 08/09/20 10:07 AM (3 years, 5 months ago) |
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Quote:
Vylie said:
Quote:
Pastywhyte said: I not really worried about Bacillus thuringiensis itself surviving the PC cycle as much as I used to. Now I’m more concerned with it’s cry toxins that it produces. These cry toxins are pretty resistant to heat, more so than the bacteria that generates them.
Aren’t they toxic only for insects?
Apparently not.
https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300027
Quote:
It can be assumed that B. thuringiensis thuringiensis 407 (pH 408) and B. thuringiensis kurstaki HD-73 strains have inhibited the micelial growth of the phytopathogenic fungi R. solani,P. grisea,F. oxysporum and F. solani, while proteins at the tested concentrations had no effect. Hence, data from this study are promising regarding the usage of strains for the biological control of the tested fungi.
Obviously not enough work has been done to say anything definitively. But it’s definitely interesting and makes me more wary of organic grain products.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26871314 - 08/09/20 10:20 AM (3 years, 5 months ago) |
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Quote:
Vylie said:
Quote:
I dont think the oat jars are going to work.
Why?
Because when I tried that the dry grains got scorched and the hydrated grains burst. It was not good.
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mushboy
modboy



Registered: 04/24/05
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Re: Mushrooms’ adventures in the frige [Re: mushboy] 1
#26871408 - 08/09/20 11:11 AM (3 years, 5 months ago) |
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No it was dry grains tossed into overly wet hydrated grains to balance it out. Or so I assumed.
The 'trick' to oats is thoroughly drying them.
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: mushboy]
#26871645 - 08/09/20 01:37 PM (3 years, 5 months ago) |
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Post deleted by Vylie
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Mushrooms’ adventures in the frige [Re: Vylie] 1
#26871652 - 08/09/20 01:43 PM (3 years, 5 months ago) |
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Stop making LC out of shitty sugary media if you don’t like it being sugar shocked. I got some potato LC going right now that’s kicking ass.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
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Re: Mushrooms’ adventures in the frige [Re: Vylie] 1
#26871695 - 08/09/20 02:16 PM (3 years, 5 months ago) |
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Quote:
Vylie said: I tasted the clear one. Yes, I drank from it a bit. It tasted like honey with water after ten days. There was no contamination in it.

What does a clean vs dirty lc taste like?
Edited by mushboy (08/09/20 02:22 PM)
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Mushrooms’ adventures in the frige [Re: mushboy]
#26871720 - 08/09/20 02:34 PM (3 years, 5 months ago) |
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Bad LC tastes like bad wine bro, dontcha know? Seriously, drinking it sounds pretty disgusting and you'd have to be a bit socially awkward to admit such a thing in public and expect no reaccion.
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MLPismyOPSEC
That One Ponyfucker


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Re: Mushrooms’ adventures in the frige [Re: Josex]
#26872027 - 08/09/20 05:49 PM (3 years, 5 months ago) |
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I was always curious what LC tasted like, but not curious enough to actually drink it. Thanks for taking that hit for me Vylie!
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CocaineBuffet
Stranger



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Posts: 3,456
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26872136 - 08/09/20 06:44 PM (3 years, 5 months ago) |
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Quote:
Vylie said:
As for the LC.
I opened the jars. I tasted the clear one. Yes, I drank from it a bit.
I am curious how you went about this in any way that wouldn't contaminate it assuming it was clean to start with.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Mushrooms’ adventures in the frige [Re: CocaineBuffet]
#26872142 - 08/09/20 06:47 PM (3 years, 5 months ago) |
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While the rest of us mere mortals are testing LC on agar to verify if it’s axenic, OP has been blessed with the ability of super taste. Sounds definitive. Case closed.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Mushrooms’ adventures in the frige [Re: Pastywhyte]
#26872323 - 08/09/20 08:51 PM (3 years, 5 months ago) |
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Every time i drink beer im like oh yea definitely a yeast infection. And of course can identify every microorganism in kombucha by taste. Thought everyone could.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Mushrooms’ adventures in the frige [Re: bodhisatta]
#26872348 - 08/09/20 09:07 PM (3 years, 5 months ago) |
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Y'all are just a bunch of inconsiderate pricks, dudes. Now the OP is gonna have to make a huge post, quoting every person who gave her shit for the LC tasting test. That's a lot of work y'all are putting on her shoulders and y'all deserve the wall of text that's coming to every one of ya.
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mushboy
modboy



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Re: Mushrooms’ adventures in the frige [Re: Josex]
#26872372 - 08/09/20 09:22 PM (3 years, 5 months ago) |
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my lc tasted really salty. i hope i drank from the right cup... it looked about right..
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Vylie
The more you know

Registered: 03/11/20
Posts: 463
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Pastywhyte]
#26872674 - 08/10/20 03:37 AM (3 years, 5 months ago) |
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26872735 - 08/10/20 05:24 AM (3 years, 5 months ago) |
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4% is a lot for an lc. Doesnt mean it cant work, but its way more than you need. Over 20 times what i use
Look how thick this one is at 0.17% LME:
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Josex
#cheat_code


Registered: 11/13/15
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Re: Mushrooms’ adventures in the frige [Re: c10h12n2o]
#26872815 - 08/10/20 07:21 AM (3 years, 5 months ago) |
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The other day there was a guy saying he just made an LC with 40+ grams of karo, wtf that's a record.
1 gram of nutes per 500ml of water (0,2%) is more than enough for LC. Any surplus won't be used by the myc IME and won't serve any purpose other than clouding the LC and making it harder to see.
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Pastywhyte]
#26875055 - 08/11/20 12:42 PM (3 years, 5 months ago) |
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Post deleted by Vylie
Reason for deletion: ...
Edited by Vylie (08/11/20 01:40 PM)
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mushboy
modboy



Registered: 04/24/05
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26875094 - 08/11/20 01:06 PM (3 years, 5 months ago) |
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...so what's a clean lc taste like?
Fyi, visual inspection(contams) of lc is almost impossible. You got to put it to agar/grain to see... BEFORE tasting it.
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: mushboy]
#26875141 - 08/11/20 01:43 PM (3 years, 5 months ago) |
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Post deleted by Vylie
Reason for deletion: ...
Edited by Vylie (08/11/20 01:45 PM)
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mushboy
modboy



Registered: 04/24/05
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26875163 - 08/11/20 01:54 PM (3 years, 5 months ago) |
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So you only tasted uninoculated sugar/potato water? Where's the fun in that??
From a sterile tek/..whatever else point of view, I'm still unsure why you bothered tasting the broth at all
Sterile LC broth should look clear with some sediment depending on amounts of nutes used.
Got pics?
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Mushrooms’ adventures in the frige [Re: mushboy]
#26875318 - 08/11/20 03:22 PM (3 years, 5 months ago) |
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mushboy is just mad bc his lc tastes salty
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Mushrooms’ adventures in the frige [Re: c10h12n2o]
#26875388 - 08/11/20 04:04 PM (3 years, 5 months ago) |
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Fermented honey still tastes of honey. They make mead from honey and yeast. And sour mead from honey yeast and lactic acid bacteria.
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* [Re: mushboy]
#26875399 - 08/11/20 04:10 PM (3 years, 5 months ago) |
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Post deleted by Vylie
Reason for deletion: ...
Edited by Vylie (08/12/20 03:20 AM)
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26875530 - 08/11/20 05:14 PM (3 years, 5 months ago) |
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Quote:
Vylie said:
Quote:
mushboy said: So you only tasted uninoculated sugar/potato water? Where's the fun in that??
So you skipped the first three pages of this thread? It’s clever. I’m not proud of it.
I tasted a ten days old LC (honey and distilled water) in which the mushroom died.
Look, I studied chemistry. I know what I can taste and what I should not. It’s what chemistry is all about.
It’s a bit similar to sex.
Okay, I can make explosives and so pure iron that starts burning by itself when it touches the air, but such things get old fast.
I quit school and picked another profession that allows me to look young and stay alive forever.
Now, there is someone in the mirror waiting for me. I need to go. I hope you excuse me.
By the way, the 80% wet 20% dry oat mix worked out well. Tomorrow, I will attach photos.
You can use the TEK for free.
Seriously though, I'm more interested in your mirror TEK and your immortality TEK. Please, edify me.
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Vylie
The more you know

Registered: 03/11/20
Posts: 463
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* [Re: Josex]
#26876270 - 08/12/20 03:33 AM (3 years, 5 months ago) |
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Post deleted by Vylie
Reason for deletion: ...
Edited by Vylie (08/12/20 04:45 AM)
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Vylie
The more you know

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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Vylie]
#26876307 - 08/12/20 04:39 AM (3 years, 5 months ago) |
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26876635 - 08/12/20 10:14 AM (3 years, 5 months ago) |
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El Chupacabra
Stoned Ape



Registered: 01/07/19
Posts: 676
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26876676 - 08/12/20 10:40 AM (3 years, 5 months ago) |
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Quote:
Let’s hope they won’t run you on the same crap Debian/Ubuntu.
I like this thread, it's a nice break from reality. I like you too Vylie but I cant have you disrespecting the penguin like that. You GNU better.
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Biscuits



Registered: 06/29/17
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Loc: In a society
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Re: Mushrooms’ adventures in the frige [Re: Vylie] 1
#26877307 - 08/12/20 06:22 PM (3 years, 5 months ago) |
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Drinking weird LC fine, having everlasting youth fine, but advertising Gentoo? That's crazy, Gentoo is dead and Arch buried it. Get with the times.
Quote:
Vylie said: It works like charm.

It might be still too wet. The next time I’ll try 7:3 ratio.
Yesterday, the third jar received one of the mycelium blocks I saved from bacteria contamination. I still have another four saved blocks. They seem healthy.
Thanks for everyone for pointing out the oat was too wet.
I want to see how these look/smell/taste when you spawn them, they might look fine now 2 days after a g2g, but we really can't tell if they worked like a charm or not yet...
If your strange demi-god job comes with that view in the second pic, I want to apply when you return to your home planet.
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Biscuits]
#26877770 - 08/13/20 01:03 AM (3 years, 5 months ago) |
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Post deleted by Vylie
Reason for deletion: ...
Edited by Vylie (08/13/20 02:54 AM)
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Vylie
The more you know

Registered: 03/11/20
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* [Re: Vylie]
#26877813 - 08/13/20 02:05 AM (3 years, 5 months ago) |
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Post deleted by Vylie
Reason for deletion: ...
Edited by Vylie (08/13/20 03:23 AM)
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Vylie
The more you know

Registered: 03/11/20
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Last seen: 2 years, 4 months
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* [Re: Vylie]
#26878245 - 08/13/20 10:40 AM (3 years, 5 months ago) |
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26878266 - 08/13/20 10:49 AM (3 years, 5 months ago) |
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Actually, you don't have mushrooms in an LC, only mushroom jizz.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26878397 - 08/13/20 11:59 AM (3 years, 5 months ago) |
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Quote:
Vylie said: The mushrooms seem dead again in the 1-2% honey. The jars look clean. I will try 0.2% with the same honey as well as another.
Most of the time, when it worked, the LC was white. Even 1% is yellow.
But how did it taste when it worked?
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Vylie
The more you know

Registered: 03/11/20
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Last seen: 2 years, 4 months
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Post deleted by Vylie
Reason for deletion: ...
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: P [Re: Vylie]
#26878813 - 08/13/20 03:32 PM (3 years, 5 months ago) |
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Quote:
Vylie said: Can we have a basic rule that we think before we write down something?
Yeah right?
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: P [Re: Vylie]
#26878816 - 08/13/20 03:34 PM (3 years, 5 months ago) |
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Mead is excellent, especially this stuff made at a meadery in Sooke on Vancouver Island.
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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I’ve only ever drank mead in that Skyrim game. I hadn’t played video games since ps2 came out, and not much then. I moved in this place that had a xbox 360 and the game. I had no natural defenses to this shit so I got sucked into a world of magic and missions and drinking mead to keep up my stamina so I could beat tf out of creatures with my broadsword.
I was up until 4am like every day until one day in mid play, before my consciousness could catch up I opened the Xbox and took the disc right out and bent it all up. I was saved. True story too
I’m just here figured I’d share that cuz someone said mead.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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You need an archer if you like to be OP as fuck in Skyrim, bro. 
Oops not cult related. Well his thread was bound to derail anyway right? might as well move it to journal land so we can get risqué with OP.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: P [Re: Josex]
#26878927 - 08/13/20 05:00 PM (3 years, 5 months ago) |
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Quote:
Josex said: You need an archer if you like to be OP as fuck in Skyrim, bro. 
Oops not cult related. Well his thread was bound to derail anyway right? might as well move it to journal land so we can get risqué with OP. 
I totally would but I still want to see if the OP is honest enough to post the pics of those oat jars later. Y’all just gonna haf to keep it in yer pants a lil longer.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Yea I for one wanna see her results among other things.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 24 days, 17 hours
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Quote:
Roger Clemency said: I was up until 4am like every day until one day in mid play, before my consciousness could catch up I opened the Xbox and took the disc right out and bent it all up. I was saved. True story too
Don't worry, you can re-buy the game for like $8 at game stop, and then proceed to get drunk and kill your thane, then kill everyone in the whole town again, and then kill all the guards when they come, and then... oh shit did I say that out loud?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Gayfish
Lyrical genius


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For some reason, I keep reading OP’s name as violet. Can’t figure out why.... ah well, back to eating grass seed covered in mycelium I go.
On topic: I got nothing to contribute here, ‘twas a fun read while pooping though
-------------------- We don’t make sense around here, we make dollars
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
#26879662 - 08/14/20 05:09 AM (3 years, 5 months ago) |
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This thread has been closed.
Reason: :lock:
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