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InvisibleCaps McGee
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Re: Mushrooms’ adventures in the frige [Re: mushboy] * 1
    #26870102 - 08/08/20 01:20 PM (3 years, 5 months ago)

If you're getting bacteria at the bottom it IS the oats... but it's due to excess moisture: this may be a prep issue, or you might be using too much liquid innoculant...  this is a stupid arguement: really ... try drying the oats better before loading them or not cooking them as long before pressure cycle, to cut back on your moisture level at the bottom... gotta minimize the conditions that breed bacteria: hot, wet, anaerobic conditions (like at the bottom of a jar/bag of grain with too much moisture present, either due to prep or over-inoculation) food for thought:  if you're going to post a thread with questions, try not arguing with the people trying to help lol

Your title is unfinished: "the more you think you know, the less you can be taught"
:rollsafe:
Wishing you the best


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InvisibleJosex
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Re: Mushrooms’ adventures in the frige [Re: Caps McGee]
    #26870107 - 08/08/20 01:24 PM (3 years, 5 months ago)

yeah oats need some serious drying to get them right IMO.


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InvisibleCaps McGee
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Re: Mushrooms’ adventures in the frige [Re: Josex]
    #26870109 - 08/08/20 01:28 PM (3 years, 5 months ago)

Dude... I've been deying them completely  on the outside, and if i don't undercook them also, the middle of the bags turn to mush... idk if its the 75X's not venting pressure vs presto or what... I'm having to relearn/redesign the whole prep process for bags... considering going back to jars so I can afford the shorter cycle
Got to thinking also, I wonder if I'm bringing them to temp to quickly? Idk, but the steam definitely penetrates much deeper into the bags: monkey wrenches me pretty good, but we're looking better now woof


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OfflineVylie
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Caps McGee]
    #26870185 - 08/08/20 02:28 PM (3 years, 5 months ago)

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OfflineVylie
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Caps McGee]
    #26870194 - 08/08/20 02:35 PM (3 years, 5 months ago)

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Edited by Vylie (08/08/20 02:36 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
    #26870199 - 08/08/20 02:39 PM (3 years, 5 months ago)

There's plenty of bacteria that can survive a normal pressure cooker cycle. But they're only found at the bottom of the ocean or other extreme places. The endospores used to validate autoclaves require a special incubator to grow just to check if they were killed or not


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InvisibleThe Fresh PrintsS
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
    #26870208 - 08/08/20 02:43 PM (3 years, 5 months ago)

Quote:

Vylie said:
If it’s coming because the oat is too wet, then it is coming from the oat. Period.



That is variable that is 100% under your control. Oat prep can be tricky, but once you get it down, it's great (and cheap!) for spawn. 👍


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InvisibleJosex
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Re: Mushrooms’ adventures in the frige [Re: bodhisatta]
    #26870209 - 08/08/20 02:44 PM (3 years, 5 months ago)

Quote:

bodhisatta said:
The endospores used to validate autoclaves require a special incubator to grow just to check if they were killed or not




That's interesting, can you say more about that or maybe a link to an article on the subject?


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InvisiblemushboyMDiscord
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
    #26870212 - 08/08/20 02:47 PM (3 years, 5 months ago)

Quote:

Vylie said:

It will always be about a race between the mushrooms and the other organisms who either got inside or survived the PC.





They only get in if you let them in. Thats the point.


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InvisiblebodhisattaMDiscordReddit
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Re: Mushrooms’ adventures in the frige [Re: Josex]
    #26870232 - 08/08/20 03:02 PM (3 years, 5 months ago)

Quote:

Josex said:
Quote:

bodhisatta said:
The endospores used to validate autoclaves require a special incubator to grow just to check if they were killed or not




That's interesting, can you say more about that or maybe a link to an article on the subject?




https://multimedia.3m.com/mws/media/497755O/attest-bi-for-steam-1262-package-insert-english.pdf



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InvisibleJosex
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Re: Mushrooms’ adventures in the frige [Re: bodhisatta]
    #26870261 - 08/08/20 03:31 PM (3 years, 5 months ago)

Aren't those the strips I've seen you talk about on some occasions? Or these are a different thing? I wish someone would try sticking one of those things in a grain jar or bag. It'd be nice to know for sure, beyond anecdotal evidence or consensus, whether or not there are surviving endos after a cycle.


Edited by Josex (08/08/20 03:39 PM)


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InvisibleCaps McGee
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Re: Mushrooms’ adventures in the frige [Re: Josex]
    #26870357 - 08/08/20 05:06 PM (3 years, 5 months ago)

It's coming from the conditions created from improper prep: period... user error brotatoe

Sab isn't sterile: but your technique should be... SAB is a STILL air box, and operates on the principle of STILL air... hence slow concise thoughtful movement and hand placement, never putting more of your self or instruments over growth media than necessary


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OfflineVylie
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* [Re: Caps McGee]
    #26870974 - 08/09/20 04:41 AM (3 years, 5 months ago)

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OfflineVylie
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Vylie]
    #26870982 - 08/09/20 04:57 AM (3 years, 5 months ago)

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InvisiblebodhisattaMDiscordReddit
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
    #26871013 - 08/09/20 05:49 AM (3 years, 5 months ago)

We don't soak grain for endospores. You will end up with more than you started with.

The window of opportunity is a nearly 4 year old quote too. I don't really believe that shit at all anymore. if you don't inoculate a jar of sterilized oats it wont contaminate even after months of sitting if you did things right.


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OfflineForresterM
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
    #26871017 - 08/09/20 05:52 AM (3 years, 5 months ago)

:whathesaid:

The idea that improper grain preparation can cause bacterial contamination is blatantly wrong.  Having the grains too wet can cause mushy grain crap at the bottoms of jars, which may look like bacterial contamination, and may be prone to it because of it's soupy nature, but it does not cause it.

If you are getting consistent bacterial contamination in jars, either you are not sterilizing properly in the first place, or your technique is suffering and the bacteria is getting in somehow.

It's that simple.

edit:  the "window of opportunity" idea can be helpful when referring to substrates spawned in open air, but that's about it.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


Edited by Forrester (08/09/20 05:53 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Mushrooms’ adventures in the frige [Re: Josex]
    #26871020 - 08/09/20 05:57 AM (3 years, 5 months ago)

Quote:

Josex said:
Aren't those the strips I've seen you talk about on some occasions? Or these are a different thing? I wish someone would try sticking one of those things in a grain jar or bag. It'd be nice to know for sure, beyond anecdotal evidence or consensus, whether or not there are surviving endos after a cycle.



Those are what I've mentioned.
The problem is they're expensive and you have to have an account from a relevant business to even buy them.

For what its worth if your pressure cooker isn't broken in 15m at 15psi or 250F a biological indicator with 10^7 hard to kill endospores will come back completely dead. But that's not in a grain jar.
I still suspect the 2 hours is more than overkill though.


Also,
@OP
If you search only to confirm your bias you won't learn shit.


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InvisiblemushboyMDiscord
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Re: Mushrooms’ adventures in the frige [Re: Vylie]
    #26871048 - 08/09/20 06:26 AM (3 years, 5 months ago)

If agar wont colonize your lc I'd suspect 2 things.

Contamination of sorts or crap lc broth. Unless you are freezing your cultures the fridge is not the culprit.

Honey LCs gave me nothing but shit performance. I've seen people use em tho. 4% is also really high. Try 1-2% max.

Ps. You couldnt pay me to g2g that chunk of cheesey looking myc. That's gonna mold. You mine as well top fruit that chunk.


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OfflineVylie
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Forrester]
    #26871055 - 08/09/20 06:32 AM (3 years, 5 months ago)

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OfflineVylie
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Re: Mushrooms’ adventures in the frige *DELETED* *DELETED* *DELETED* *DELETED* [Re: Vylie]
    #26871072 - 08/09/20 06:52 AM (3 years, 5 months ago)

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Edited by Vylie (08/09/20 06:57 AM)


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