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Offlinesendmehummus
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Homemade LC: Lingering Questions
    #26867944 - 08/07/20 09:11 AM (3 years, 6 months ago)

Hey all ๐Ÿ™‚. So I read an amazing tutorial on here for making LC. I couldnt post or respond on that actual post for some reason (could it being 14 yrs old have anything to do with that ๐Ÿ˜…). I just had some simple questions lingering after reading every single comment for answers. 1) How long and what pressure do you sterilize syringes at in a PC? Do you take them apart or just toss em in? 2) What is the easiest/ best/ mostly sterile way to collect a spore print?  Thanks to anyone willing to educate a newb!


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InvisibleRoger Clemency
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Re: Homemade LC: Lingering Questions [Re: sendmehummus]
    #26868014 - 08/07/20 09:55 AM (3 years, 6 months ago)

If it was 14 yo thereโ€™s a good chance of bad info. Do you have a link to it?

For LC I just put 500ml water in a qt jar with 6-8g of light malt extract. You could use another type of sugar source, and then PC with my grain jars, inoculate with a clean wedge of agar and when thebLC is ready I swirl it up and pour it into other qts. The only time I used a syringe with a SHIP I contaminated the LC. I much prefer pouring.

As to sterilizing syringes and taking prints there are a few teks on here but making an LC with spores is asking for trouble, you really want to start LC with a clean wedge or maybe the Josex* โ€œpoke methodโ€


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Offlinesendmehummus
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Re: Homemade LC: Lingering Questions [Re: Roger Clemency]
    #26868042 - 08/07/20 10:10 AM (3 years, 6 months ago)

Right on thanks for the input!

https://www.shroomery.org/forums/showflat.php/Number/5238137/fpart/1/vc/1

Thats the post I was referring to. Ive been using agar for awhile and just really prefer to use syringes with my setup, so much easier. I wouldnt mind just using plates to make LC though, at least Ill be using less than inoculating Qts with wedges. Honestly I never considered just pouring to inoculate haha.. very interesting. Im curious how long you have to PC a bunch of used syringes though, its hard to get a solid answer on that. Thanks again for the advice ๐Ÿ‘.


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Invisiblemicelio
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Re: Homemade LC: Lingering Questions [Re: sendmehummus]
    #26868268 - 08/07/20 12:26 PM (3 years, 6 months ago)

Here's some info on a method of making lc.

You'll find allot of useful knowledge...

Pastywhyte's EzLC


https://www.shroomery.org/forums/showflat.php/Number/23740857


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Offlinesendmehummus
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Re: Homemade LC: Lingering Questions [Re: micelio]
    #26868315 - 08/07/20 12:55 PM (3 years, 6 months ago)

Niccce, thanks dude! Excited to start making my own.. fingers crossed ๐Ÿคž


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