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Offlineshortbull
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Did I wait too long to inoculate?
    #26867324 - 08/06/20 08:51 PM (3 years, 5 months ago)

I sterilized some BRF jars in a pressure cooker about 6 days ago but didn't have time to inoculate them. Do I need to start over or can I just re-sterilize the BRF that is already in the jars?


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InvisibleSir Pentinite
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Re: Did I wait too long to inoculate? [Re: shortbull]
    #26867339 - 08/06/20 08:56 PM (3 years, 5 months ago)

Did you keep foil over the lids?


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Offlineshortbull
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Re: Did I wait too long to inoculate? [Re: Sir Pentinite]
    #26867345 - 08/06/20 09:00 PM (3 years, 5 months ago)

Yes. Foil has remained on. Transpore tape over holes too.


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InvisibleSir Pentinite
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Re: Did I wait too long to inoculate? [Re: shortbull]
    #26867357 - 08/06/20 09:10 PM (3 years, 5 months ago)

You have the foil, the tape, and the vermiculite layer still there after a PC cycle, so I would think they're still good to use.

If the contents are truly sterile, then they should last indefinitely or at least until they dry out.

I mean it's not good practice though.


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"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


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Offlineshortbull
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Re: Did I wait too long to inoculate? [Re: Sir Pentinite]
    #26867362 - 08/06/20 09:12 PM (3 years, 5 months ago)

Quote:

I mean it's not good practice though.




Yeah, it is nagging at me that it would be really easy to make a new batch. Still, I have some excess spores so maybe it is worth experimenting.


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Offlineskullhuman
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Re: Did I wait too long to inoculate? [Re: shortbull]
    #26867458 - 08/06/20 10:32 PM (3 years, 5 months ago)

They've been in the PC the whole time?


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Offlineshortbull
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Re: Did I wait too long to inoculate? [Re: skullhuman]
    #26867730 - 08/07/20 06:52 AM (3 years, 5 months ago)

Quote:

They've been in the PC the whole time?




No. I took them out of the PC and put them in a closet.


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OfflineItsaMeShroomio
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Re: Did I wait too long to inoculate? [Re: shortbull]
    #26867769 - 08/07/20 07:27 AM (3 years, 5 months ago)

If they're not your last spores I would go for it. 6 days is not that long, considering it takes a month or so to fully colonize.


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InvisibleRoger Clemency
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Re: Did I wait too long to inoculate? [Re: shortbull]
    #26867774 - 08/07/20 07:31 AM (3 years, 5 months ago)

6 days is fine, the jars take a month or sometimes more to be ready so an extra couple days won’t hurt.

If they had been sitting with just the dry vermiculite layer for protection then maybe when you inoculated you’d shake some mold spores loose that the verm had caught but I think you aiight.


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OfflineNichrome
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Re: Did I wait too long to inoculate? [Re: ItsaMeShroomio]
    #26867777 - 08/07/20 07:32 AM (3 years, 5 months ago)

They're probably fine. Spores are cheap/free and materials are cheap too. worst case scenario you grow some nasty contams. If nothing has grossed them out yet its not likely they are bad.


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Offlineshortbull
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Re: Did I wait too long to inoculate? [Re: Nichrome]
    #26867821 - 08/07/20 08:01 AM (3 years, 5 months ago)

Thanks to all who responded. Let the experiment begin.


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Offlineskullhuman
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Re: Did I wait too long to inoculate? [Re: shortbull]
    #26868039 - 08/07/20 10:08 AM (3 years, 5 months ago)

It'll likely work fine.


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InvisibleThe Blind Ass
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Re: Did I wait too long to inoculate? [Re: skullhuman]
    #26868582 - 08/07/20 03:07 PM (3 years, 5 months ago)

I’ve waited roughly 10+ days before inoculating.  Worked fine.


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Offlineskullhuman
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Re: Did I wait too long to inoculate? [Re: The Blind Ass]
    #26869194 - 08/07/20 09:34 PM (3 years, 5 months ago)

Yeah, if it's prepped properly the jar will sit and grow nothing for months.


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