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Offlineemm8055
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Contamination on this AGAR dish?
    #26866294 - 08/06/20 11:27 AM (3 years, 5 months ago)

Tried to use a spore print....is this a goner?



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OfflineBloob
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Re: Contamination on this AGAR dish? [Re: emm8055]
    #26866355 - 08/06/20 11:52 AM (3 years, 5 months ago)

The Mycelium could win, seems to have somewhat of a hold. But it looks pretty contaminated


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Offlineemm8055
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Re: Contamination on this AGAR dish? [Re: Bloob]
    #26866395 - 08/06/20 12:23 PM (3 years, 5 months ago)

Thanks for replying.  I will give it a couple more days.


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OfflineAlsetAlokin
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Re: Contamination on this AGAR dish? [Re: emm8055]
    #26866405 - 08/06/20 12:33 PM (3 years, 5 months ago)

Following this to learn what's going on in this plate.

Do you have any others to compare it to or is this the only plate you put spores to?

I know germination plates can get pretty wild looking but (from my untrained eye) I don't see much good going on.


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Offlineemm8055
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Re: Contamination on this AGAR dish? [Re: AlsetAlokin]
    #26867363 - 08/06/20 09:13 PM (3 years, 5 months ago)

I did six plates....2 of each (3 different types).  What you see is BAM and both plates look like this.


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Offlineemm8055
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Re: Contamination on this AGAR dish? [Re: emm8055]
    #26872077 - 08/09/20 06:18 PM (3 years, 5 months ago)

Here is an update on all the dishes I made....the original one I posted a photo of did not make it.  Started getting really funky so I tossed it.  This photo is day 4.  Would like some opinions on how we are looking here.

From top to bottom,

Bam 1
Bam 2 (does this look ok)
GT 1 (have serious doubts about this one)
GT 2
Albino A+ 1
Albino A+ 2
Maza (concerned about this one....this looks better than the one in the the first thread that I tossed)

The Albino was the only one not made from a spore print....I used a syringe on that.  Doesn't look like either one is reacting at all.

Thanks for everyones help.



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Offlineemm8055
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Re: Contamination on this AGAR dish? [Re: emm8055]
    #26872290 - 08/09/20 08:31 PM (3 years, 5 months ago)

Bump


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InvisibleJosex
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Re: Contamination on this AGAR dish? [Re: emm8055]
    #26872302 - 08/09/20 08:42 PM (3 years, 5 months ago)

You said those are germination plates right? I'd toss those and start over then.

They look terrible and if there's cube myc in there is gonna be meshed in with mold myc, and cleaning that kinda shit is something really difficult that can take an experienced grower a lot of time and patience to clean, if ever.

It's really not worth your effort to try and clean those, they're just germ plates, not some really good culture you can't afford to lose.


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OfflineGan
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Re: Contamination on this AGAR dish? [Re: Josex]
    #26872309 - 08/09/20 08:44 PM (3 years, 5 months ago)

:whathesaid:

Did you streak your spores?


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InvisibleJosex
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Re: Contamination on this AGAR dish? [Re: Gan]
    #26872376 - 08/09/20 09:24 PM (3 years, 5 months ago)

Yea I'd like to know how you put those spores on the agar.

Also, given that you're working with several prints, the odds are slim of all of them being that nasty.

I'm more inclined to believe that the thing that's giving you trouble are the plates themselves, they look really bad for agar, so flimsy and that type of lid they have don't offer much protection. How are you filtering those plates btw?

Could be bad technique too. Explain your process a bit.


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Offlineemm8055
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Re: Contamination on this AGAR dish? [Re: Josex]
    #26872505 - 08/09/20 10:55 PM (3 years, 5 months ago)

I use a scalpel to scrape the spore print and drop it on.


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Offlineskullhuman
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Re: Contamination on this AGAR dish? [Re: emm8055]
    #26872520 - 08/09/20 11:31 PM (3 years, 5 months ago)

Is it sterilized beforehand?


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Offlineemm8055
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Re: Contamination on this AGAR dish? [Re: skullhuman]
    #26873583 - 08/10/20 03:41 PM (3 years, 5 months ago)

Yes, I use a small torch to sterilize it.  I'm wondering if the sporeprints I made are bad.  Over time, I've been way more conscious of the working conditions and sterilizing my area.  I dropped the spores onto the AGAR in front of a flow hood in a sterilized room.

I love mycology but man can it be frustrating.


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Offlineemm8055
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Re: Contamination on this AGAR dish? [Re: emm8055]
    #26873596 - 08/10/20 03:52 PM (3 years, 5 months ago)

And I was pretty conscious while making the AGAR.  Followed instructions and used a PC.


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OfflineEp1429
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Re: Contamination on this AGAR dish? [Re: emm8055]
    #26873602 - 08/10/20 03:54 PM (3 years, 5 months ago)

Quote:

emm8055 said:
Yes, I use a small torch to sterilize it.  I'm wondering if the sporeprints I made are bad.  Over time, I've been way more conscious of the working conditions and sterilizing my area.  I dropped the spores onto the AGAR in front of a flow hood in a sterilized room.

I love mycology but man can it be frustrating.




Use a sterile q-tip.  You can buy them at Walgreens or Rite-Aid.  Swab the spores, lightly drag across the agar in a zigzag.  I also find it’s better to use fresh spores, they seem to take better.

But switching to the q-tip was the difference between success and failure for me, when starting with spores on agar.



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Offlineemm8055
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Re: Contamination on this AGAR dish? [Re: Ep1429]
    #26873623 - 08/10/20 04:09 PM (3 years, 5 months ago)

Awesome....thank you for the tip.  Definitely going to do that.


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Offlineemm8055
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Re: Contamination on this AGAR dish? [Re: emm8055]
    #26873666 - 08/10/20 04:33 PM (3 years, 5 months ago)

Before I toss these, just want to make sure I'm not throwing out something that would work.



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OfflineEp1429
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Re: Contamination on this AGAR dish? [Re: emm8055] * 1
    #26873685 - 08/10/20 04:47 PM (3 years, 5 months ago)

Let them go,
let them go!

Turn around,
and slam the door!


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OfflineBroken Veils
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Re: Contamination on this AGAR dish? [Re: emm8055]
    #26873846 - 08/10/20 06:28 PM (3 years, 5 months ago)

2nd pic - 9-11 o'clock on the bottom growth could be workable with time...outside of that toss and start over


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Offlineemm8055
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Re: Contamination on this AGAR dish? [Re: Broken Veils]
    #26873858 - 08/10/20 06:37 PM (3 years, 5 months ago)

Thanks....I was thinking the same thing.


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