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Offlineseand04
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Liquid culture
    #26865315 - 08/05/20 07:09 PM (3 years, 5 months ago)

I am going to inoculate a lot of jars so I made this jar of water potato flakes and honey. I am planning on sterilizing the liquid then using a syringe to inoculate the jar, wait for growth then use it to inoculate all my grain jars. If anyone has any pointers let me know. Thanks


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Offlinelateforthafuture
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Re: Liquid culture [Re: seand04]
    #26865346 - 08/05/20 07:25 PM (3 years, 5 months ago)

I would try and isolate some clean growth on agar first...then drop an agar wedge in the liquid culture mix, instead of injecting straight spore solution...thats my pointer.

At the very least, if you can't or won't do agar, then at least test the liquid culture with a pint jar of grain first...before you go noccing up a bunch of quart jars.

Spore solution > liquid culture is risky business. Not that it can't work...but tread lightly.


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"Not what could have been, or what should have been, but what will be" -Self
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Offlineseand04
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Re: Liquid culture [Re: lateforthafuture]
    #26865355 - 08/05/20 07:29 PM (3 years, 5 months ago)

Awesome.  I make agar plates. I was  thinking about making a lot of jars at once. Without waiting for an agar plate. I  was going to use a spore print I have in my SAB to inoculate this jar. Now I wonder if it maybe too risky though ๐Ÿค”. Now I am thinking to do the agar plates.  Question tho should I  make a syringe and put it on the plates or scrape the actual spores directly onto the agar plates?


Edited by seand04 (08/05/20 07:48 PM)


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Re: Liquid culture [Re: seand04]
    #26865387 - 08/05/20 07:53 PM (3 years, 5 months ago)

If I were you I would go

Spore print > agar plate > transfer to at least one more plate > liquid culture > grain jar.

No need to make a syringe...that's actually counter productive in your case. I assumed you were using a syringe..didn't know you had a print.

print>agar>LC>grain is the route I would go.

Waiting for the agar will save you time in the long run...if the spores > LC fails...you'll have to start over anyways...just put it to agar first and be done with it..IMHO.


--------------------
"Not what could have been, or what should have been, but what will be" -Self
:superbanana::mjdance::dancer:


Edited by lateforthafuture (08/05/20 07:54 PM)


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Offlineseand04
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Re: Liquid culture [Re: lateforthafuture]
    #26865390 - 08/05/20 07:54 PM (3 years, 5 months ago)

Thanks a million brother.  Thats what I am gonna do. One last question.  Should I refrigerate the Liquid jars I made then sterilize them when the agar is ready? Or just toss em? I was thinking it will be at least a week for the agar.


Edited by seand04 (08/05/20 07:59 PM)


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Re: Liquid culture [Re: seand04]
    #26865419 - 08/05/20 08:13 PM (3 years, 5 months ago)

I would just make a fresh jar of liquid culture mix when your plates are ready. If you've already cooked the liquid culture you don't want to cook it again... it will carmelize the sugars and you don't want that. Also, you just want your recieving jars (grains, lc, etc) to be a fresh as possible when you inoculate...it just betters your chances at success.

Liquid culture is cheap as dirt to make... your mycelium will appreciate the clean, fresh liquid media.

Just like the food we eat..the mushies want fresh food :smile:


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"Not what could have been, or what should have been, but what will be" -Self
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Re: Liquid culture [Re: lateforthafuture]
    #26865447 - 08/05/20 08:32 PM (3 years, 5 months ago)

Awesome thanks for the great tips๐Ÿ™ I am thinking about using a bit of this jar for the agar plates.  It will be faster probably. I haven't tried this yet, but I was thinking about shaking the jar, wiping it down with iso then use a flamed exacto knife to put the mycelium on the agar plates.


Edited by seand04 (08/05/20 08:50 PM)


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Offlineseand04
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Re: Liquid culture [Re: seand04]
    #26865476 - 08/05/20 08:52 PM (3 years, 5 months ago)

This jar.


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Re: Liquid culture [Re: seand04]
    #26865847 - 08/06/20 05:59 AM (3 years, 5 months ago)

Quote:

seand04 said:
This jar.




That's a good idea...that jar looks pretty clean too... that will def speed things up... I've put grain to agar many times with good success.


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"Not what could have been, or what should have been, but what will be" -Self
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Re: Liquid culture [Re: lateforthafuture]
    #26866128 - 08/06/20 10:12 AM (3 years, 5 months ago)

Sweet. Thats what I am going to do.


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Offlineseand04
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Re: Liquid culture [Re: seand04]
    #26866198 - 08/06/20 10:50 AM (3 years, 5 months ago)

Made the plates. Mad props to pastywhyte, with the easy agar plate tek.


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Re: Liquid culture [Re: seand04]
    #26866239 - 08/06/20 11:05 AM (3 years, 5 months ago)

Shit looks deep bruh.


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Re: Liquid culture [Re: El Chupacabra]
    #26866261 - 08/06/20 11:16 AM (3 years, 5 months ago)

Deep? Should I make them.more shallow?


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Re: Liquid culture [Re: seand04]
    #26866314 - 08/06/20 11:35 AM (3 years, 5 months ago)

Idk it's hard to say but the angle makes it look like half filled containers.

Just a bit more than a fully covered bottom is perfect. Much more than that and it gets difficult to take transfers from.
Half filled containers would make for an interesting tiger drop tho.


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Re: Liquid culture [Re: El Chupacabra]
    #26866337 - 08/06/20 11:44 AM (3 years, 5 months ago)

Ok ok. They are about halfway filled. Will that make them more susceptible to contams?


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Re: Liquid culture [Re: seand04]
    #26866438 - 08/06/20 12:50 PM (3 years, 5 months ago)

Nope, no more of a contam risk at all just a potential PITA down the line.


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