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OfflineTony Shroomprano
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Registered: 07/31/20
Posts: 7
Last seen: 2 days, 19 hours
First time doing agar with pasty plates, need a second opinion on potential contams
    #26864987 - 08/05/20 04:45 PM (3 years, 5 months ago)

Hey everybody, so first of all this is my first post. Second of all, this is my first time doing agar. I'm doing pasty plates with the tek by Pastywhyte and doing Tiger Drop. I have 14 plates inoculated and all but 2 have taken off, with each plate having a varying growth rate. While most of them look good to me, there's 4 I'm concerned about and need a second opinion. I apologize for the picture quality I tried my best :/.

So here's what most of my plates look like:

Nice, simple spreading circle of mycelium with no signs of anything else as far as I can tell.

However, I have a few problem plates. Here's #1:


I'm not sure if I'm looking at tomentose mycelium growth or cobweb. Need help with identification.

Problem plate #2:


I don't like how it isn't a proper circle and seems to have multiple points of mycelium spread, and looks to possibly have cobweb.

Problem plate #3:


Same issue as #2, also appears to be a second mycelium colony on the side of the plate, is possibly mold but I'm not 100% sure.

Problem plate #4:


So this one appears to be fine on the surface, however as you can see in the second pic, the bottom appears to have these "dry patches". None of my other plates have them, and I'm wondering if its just a place where the agar doesn't touch the side of the plate or if its some sort of bacterial contamination.

I know the post was long, but if somebody can give me a second opinion on my 4 problem plates, that'd be swell. Thanks!


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