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OfflineMr. Cube
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Bulk tubs look a little fuzzy on top
    #26864979 - 08/05/20 04:40 PM (3 years, 5 months ago)

hey whats up.
Check out this this tub and let me know what you think. Pins fruits and contams? Tubs made from grain spawn 2 weeks ago.


Edited by Mr. Cube (08/05/20 04:46 PM)


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26864981 - 08/05/20 04:41 PM (3 years, 5 months ago)

trying to figure out how to add pictures


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26865920 - 08/06/20 07:27 AM (3 years, 5 months ago)

I can post more pictures if needed. Was wondering if its contam or trich. Bruises blue if touched so I'm wondering if its just fluffy mycelium.


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InvisibleMadHatter333
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26865997 - 08/06/20 08:35 AM (3 years, 5 months ago)

Looks like the beginning stages of trich to me. If you have any other grows going right now I’d quarantine that one before it goes green. I may be mistaken and it could be bacteria but it looks like it will turn into the mean green soon.


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Offlineshroomrookie
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26866122 - 08/06/20 10:08 AM (3 years, 5 months ago)

Looks good to me. I've seen all those colors early on with success but you never know. All precautions are good though and if others see contamination then you may want to isolate that grow.


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: MadHatter333]
    #26866150 - 08/06/20 10:23 AM (3 years, 5 months ago)

Hey thanks. Only grow right now. Just not sure what to do. Fruits geting bigger by the hour lol. It was buket tech 1 to 1 with rye grain spawn.


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: shroomrookie]
    #26866155 - 08/06/20 10:25 AM (3 years, 5 months ago)

isolate grow on agar. Would love to keep type. How many agar plates is nessasary to isolate?


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Offlineshroomrookie
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26866182 - 08/06/20 10:42 AM (3 years, 5 months ago)

Not a question I can answer. I only started with agar in 2019. But I'm positive if you search your question you will have hours of experience to read about.


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OfflineGastronomicus
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Re: Bulk tubs look a little fuzzy on top [Re: shroomrookie]
    #26866191 - 08/06/20 10:47 AM (3 years, 5 months ago)

If it's your only tub just leave it for a day or two and see if it turns green. When shtoomrookie said isolate it he meant put the tub somewhere by itself, not isolate the strain on agar. Don't worry about doing that yet just focus on getting clean spawn.


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Offlinenewtomyc
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26866193 - 08/06/20 10:49 AM (3 years, 5 months ago)

Quote:

Mr. Cube said:
isolate grow on agar. Would love to keep type. How many agar plates is nessasary to isolate?




Check this thread out.  Closest I have seen documented isolate.

https://www.shroomery.org/forums/favlinker.php?Cat=&Entry=180127&F_Board=2&Thread=24741888&partnumber=&postmarker=


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: newtomyc]
    #26866241 - 08/06/20 11:05 AM (3 years, 5 months ago)

Hey thanks for responses. I used to grow back in the day just starting up again. Do people just throw out bad tubs?


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OfflineMLPismyOPSEC
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Re: Bulk tubs look a little fuzzy on top [Re: newtomyc]
    #26866243 - 08/06/20 11:07 AM (3 years, 5 months ago)

I'm not totally sold on trich, it does look suspect. But at the very least, the orange/brown coloring indicated bacteria. If it turns green, toss it. You'll know in 24-48 hours.

About bad tubs; Yep! i toss em as soon as i see trich. They're fine to go into regular garbage, but if you have some way of composting them or putting them out with grass clippings :thumbup:


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: MLPismyOPSEC]
    #26866266 - 08/06/20 11:17 AM (3 years, 5 months ago)

Ok i wait a couple days and repost update. If it is trich I want to figure out where I went wrong. Research says trich is just from the environment and exposed spawn is subject to infection.


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26870160 - 08/08/20 02:10 PM (3 years, 5 months ago)

Ok so its been a couple days and really no green. Please take a look at new pictures. I'm not really so sure about this batch.



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Offlinejcm4620
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26870236 - 08/08/20 03:04 PM (3 years, 5 months ago)

looks like a bacterial issue to me. did u knock up your grains with a spore syringe??


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: jcm4620]
    #26870242 - 08/08/20 03:10 PM (3 years, 5 months ago)

Yes thats how I did it maybe syringe or grain was bad?


Edited by Mr. Cube (08/08/20 03:10 PM)


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26870291 - 08/08/20 03:57 PM (3 years, 5 months ago)

I know agar is the best way to get clean spawn but I'm not really in the market for a flow hood right now. Even a small one. Still air box could be ok i guess.


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OfflineMTManiac
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26870382 - 08/08/20 05:37 PM (3 years, 5 months ago)

Quote:

Mr. Cube said:
I know agar is the best way to get clean spawn but I'm not really in the market for a flow hood right now. Even a small one. Still air box could be ok i guess.



Join me in my adventure into agar :smile:
I don't have the space for a flow hood, but since this is for science and fun (and my batting average is horrible for syring to grain) I decided I could only do better.


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: MTManiac]
    #26870417 - 08/08/20 06:05 PM (3 years, 5 months ago)

Quote:

MTManiac said:
Quote:

Mr. Cube said:
I know agar is the best way to get clean spawn but I'm not really in the market for a flow hood right now. Even a small one. Still air box could be ok i guess.



Join me in my adventure into agar :smile:
I don't have the space for a flow hood, but since this is for science and fun (and my batting average is horrible for syring to grain) I decided I could only do better.




Yea i will give it a shot


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Offlinedr s
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26870426 - 08/08/20 06:09 PM (3 years, 5 months ago)

On the topic of agar, I just started myself, using a still air box and advice posted on this site - out of 50 plates I have messed with I only got 1 (possibly 2 at most) contams - it's really not as complicated as it may seem, just need to follow procedures in the guide posts about it


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InvisibleMadHatter333
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Re: Bulk tubs look a little fuzzy on top [Re: dr s]
    #26871160 - 08/09/20 08:26 AM (3 years, 5 months ago)

I use a SAB as well and have for my whole agar work experience. I have a light that I put on top of my SAB and it helps to see things more clearly. Don’t use not having a flow hood as an excuse not to do agar. It’s the way to go bro :super:


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: dr s]
    #26871180 - 08/09/20 08:40 AM (3 years, 5 months ago)

Quote:

dr s said:
On the topic of agar, I just started myself, using a still air box and advice posted on this site - out of 50 plates I have messed with I only got 1 (possibly 2 at most) contams - it's really not as complicated as it may seem, just need to follow procedures in the guide posts about it




What guide did you follow for agar? Also, if I have a 10 cc syringe that would probably make a ton of agar plates.


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: MadHatter333]
    #26871185 - 08/09/20 08:43 AM (3 years, 5 months ago)

Quote:

MadHatter333 said:
I use a SAB as well and have for my whole agar work experience. I have a light that I put on top of my SAB and it helps to see things more clearly. Don’t use not having a flow hood as an excuse not to do agar. It’s the way to go bro :super:




Then you go to agar to grain bag or jars are prolly easier?


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InvisibleMadHatter333
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26871196 - 08/09/20 08:51 AM (3 years, 5 months ago)

Yes. You clean up your plates, take the best section And transfer to another plate until you get a clean uniform plate. When you know it’s good you cut wedges out and drop them into grain jars or bags. It’s super easy! I recommend trying this to start...

https://www.shroomery.org/forums/showflat.php/Number/19208976


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OfflineMLPismyOPSEC
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Re: Bulk tubs look a little fuzzy on top [Re: MadHatter333]
    #26871941 - 08/09/20 05:10 PM (3 years, 5 months ago)

I agree it looks bacterial, but they're coming along pretty well! About a SAB, get the biggest one you can find. Sterilite makes a 160L one, it's something like $25. Here's mine, each arm hole can fit a quart jar through it with ease.



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Offlinedr s
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26875499 - 08/11/20 04:59 PM (3 years, 5 months ago)

I think I was mostly using Bod's guides, he has some videos as well as text explanation, you can look in his signature. But pretty much just made the agar, poured the plates and then inoculated with MS syringe - and yes I had 10cc syringes but you only put 1 drop on each plate, otherwise you will have a lot of excess moisture on the dish. A couple plates I did several drops and it made no difference in terms of getting mycelium on the plate or not - I had several varieties and I think I did 20 plates from the syringe, only 5 of those never grew anything all the others had mycelium, which i then transferred to new plates to make sure they were clean, before using on grain


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: dr s]
    #26876910 - 08/12/20 01:27 PM (3 years, 5 months ago)

Ok so here is another update on the tubs. Each picture is another tub. One looks better than the other.

Let me know what yall think. What cause fuzzy stems. Low FAE?


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OfflineMr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
    #26877164 - 08/12/20 04:49 PM (3 years, 5 months ago)

Anyone have a opinion on my tubs?


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