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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
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Re: Bulk tubs look a little fuzzy on top [Re: dr s]
#26871160 - 08/09/20 08:26 AM (3 years, 5 months ago) |
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I use a SAB as well and have for my whole agar work experience. I have a light that I put on top of my SAB and it helps to see things more clearly. Don’t use not having a flow hood as an excuse not to do agar. It’s the way to go bro
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Mr. Cube
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Registered: 08/05/20
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Last seen: 3 years, 5 months
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Re: Bulk tubs look a little fuzzy on top [Re: dr s]
#26871180 - 08/09/20 08:40 AM (3 years, 5 months ago) |
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Quote:
dr s said: On the topic of agar, I just started myself, using a still air box and advice posted on this site - out of 50 plates I have messed with I only got 1 (possibly 2 at most) contams - it's really not as complicated as it may seem, just need to follow procedures in the guide posts about it
What guide did you follow for agar? Also, if I have a 10 cc syringe that would probably make a ton of agar plates.
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Mr. Cube
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Re: Bulk tubs look a little fuzzy on top [Re: MadHatter333]
#26871185 - 08/09/20 08:43 AM (3 years, 5 months ago) |
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Quote:
MadHatter333 said: I use a SAB as well and have for my whole agar work experience. I have a light that I put on top of my SAB and it helps to see things more clearly. Don’t use not having a flow hood as an excuse not to do agar. It’s the way to go bro 
Then you go to agar to grain bag or jars are prolly easier?
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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
#26871196 - 08/09/20 08:51 AM (3 years, 5 months ago) |
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Yes. You clean up your plates, take the best section And transfer to another plate until you get a clean uniform plate. When you know it’s good you cut wedges out and drop them into grain jars or bags. It’s super easy! I recommend trying this to start...
https://www.shroomery.org/forums/showflat.php/Number/19208976
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
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Re: Bulk tubs look a little fuzzy on top [Re: MadHatter333]
#26871941 - 08/09/20 05:10 PM (3 years, 5 months ago) |
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I agree it looks bacterial, but they're coming along pretty well! About a SAB, get the biggest one you can find. Sterilite makes a 160L one, it's something like $25. Here's mine, each arm hole can fit a quart jar through it with ease.
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dr s
Stranger

Registered: 07/04/20
Posts: 42
Last seen: 2 years, 9 months
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
#26875499 - 08/11/20 04:59 PM (3 years, 5 months ago) |
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I think I was mostly using Bod's guides, he has some videos as well as text explanation, you can look in his signature. But pretty much just made the agar, poured the plates and then inoculated with MS syringe - and yes I had 10cc syringes but you only put 1 drop on each plate, otherwise you will have a lot of excess moisture on the dish. A couple plates I did several drops and it made no difference in terms of getting mycelium on the plate or not - I had several varieties and I think I did 20 plates from the syringe, only 5 of those never grew anything all the others had mycelium, which i then transferred to new plates to make sure they were clean, before using on grain
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Mr. Cube
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Registered: 08/05/20
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Re: Bulk tubs look a little fuzzy on top [Re: dr s]
#26876910 - 08/12/20 01:27 PM (3 years, 5 months ago) |
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Ok so here is another update on the tubs. Each picture is another tub. One looks better than the other. 
Let me know what yall think. What cause fuzzy stems. Low FAE?
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Mr. Cube
Stranger


Registered: 08/05/20
Posts: 15
Last seen: 3 years, 5 months
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Re: Bulk tubs look a little fuzzy on top [Re: Mr. Cube]
#26877164 - 08/12/20 04:49 PM (3 years, 5 months ago) |
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Anyone have a opinion on my tubs?
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