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El Chupacabra
Stoned Ape



Registered: 01/07/19
Posts: 676
Loc: 🇺🇸
Last seen: 1 year, 1 month
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26851304 - 07/29/20 07:47 AM (3 years, 6 months ago) |
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This whole thing gave me a raging hard clue lol. Clean plates to play with is definitely the silver lining here (play a little less messy next time) Watch some videos of tissue cloning and spore printing and shit like that and keep on documenting and reading here and asking questions and you'll be dehydrating big ole yields in no time.
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Infiniteshot
Stranger


Registered: 03/10/16
Posts: 268
Last seen: 2 years, 11 months
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Re: Laminar flow hood filter damage. Hepa me! [Re: El Chupacabra]
#26851324 - 07/29/20 07:56 AM (3 years, 6 months ago) |
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Totally. My plates appear to be clean except for my poor agar mixing. The plates I did ten days ago and left sealed are all clean and non contaminated. I’ve got some projects going that should produce in the next 5 weeks or so. I’ll get to some experiments at that point. It will give me plenty of time to eyeball those plates and make sure they’re perfect. Thanks for the input.
-------------------- rad
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Infiniteshot
Stranger


Registered: 03/10/16
Posts: 268
Last seen: 2 years, 11 months
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26851723 - 07/29/20 12:30 PM (3 years, 6 months ago) |
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And one more thing...I ordered a hot wire anemometer and got it up to the face of the hepa filter for a reading. It was reading 104-112. Sometimes it dropped to 96. It’s substantially more sensitive than the vane anemometer. That reading held at 6 inches out and then fell off. When reading FPM , what distance away from the filter do you advise should be used ?
-------------------- rad
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26852024 - 07/29/20 02:39 PM (3 years, 6 months ago) |
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~6" because that where you will be working.
Don't work directly next to or close to the surface of the filter, the air needs room to make a laminar flow.
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Infiniteshot
Stranger


Registered: 03/10/16
Posts: 268
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26852231 - 07/29/20 04:29 PM (3 years, 6 months ago) |
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More info. So I pulled up the anemometer and started testing at 6” distance from the face. The readings across the bottom are at about 115 FPM (all readings are FPM) at the middle they’re about 90 , and towards the top it hits 110. This is with the prefilter on and that could be removed. Everything increases by about 10 when removed. The question is would it be better to have the middle a bit low or to keep prefilter off while working and have everything high? Thanks again.
-------------------- rad
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26852278 - 07/29/20 04:45 PM (3 years, 6 months ago) |
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It sounds like your flowhood is functioning about as good as a homemade DIY flowhood will function. 90-110 is a good range.
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Infiniteshot
Stranger


Registered: 03/10/16
Posts: 268
Last seen: 2 years, 11 months
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26852309 - 07/29/20 04:58 PM (3 years, 6 months ago) |
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Thanks for the help. Have a great day
-------------------- rad
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Infiniteshot
Stranger


Registered: 03/10/16
Posts: 268
Last seen: 2 years, 11 months
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26859069 - 08/02/20 02:36 PM (3 years, 6 months ago) |
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Here’s another I did last week. I feel a bit better about this one.
-------------------- rad
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26859106 - 08/02/20 03:04 PM (3 years, 6 months ago) |
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protip: cut your parafilm in half lengthwise to get twice the parafilm and none of the obstruction.
You only need to seal that little gap at the bottom.
Looks good man.
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Infiniteshot
Stranger


Registered: 03/10/16
Posts: 268
Last seen: 2 years, 11 months
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26859112 - 08/02/20 03:07 PM (3 years, 6 months ago) |
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Thanks. Am I correct in thinking that on the left side it looks a little “wispy” and needs to be isolated?
-------------------- rad
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Laminar flow hood filter damage. Hepa me! [Re: Infiniteshot]
#26859117 - 08/02/20 03:09 PM (3 years, 6 months ago) |
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yea keep going for isolation id take a bunch of transfers around 2-3-4 o'clock
Edited by sandman420 (08/02/20 03:10 PM)
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Infiniteshot
Stranger


Registered: 03/10/16
Posts: 268
Last seen: 2 years, 11 months
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Re: Laminar flow hood filter damage. Hepa me! [Re: sandman420]
#26864969 - 08/05/20 04:34 PM (3 years, 6 months ago) |
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Hey there. So I isolated these sections of the previous plates. This is my final result. Should I keep isolating pieces or does this look cleaned up? Thanks for your time.
-------------------- rad
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