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OfflineTrippingBillies3
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Registered: 07/06/08
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First time grower using liquid culture, have a few questions
    #8600583 - 07/06/08 12:14 PM (15 years, 7 months ago)

Hello everyone! My names Chris, Im fairly new to mycology. Its been an ongoing study of mine for the past couple of months, with trying to gather all of the materials and information. Let me start off by saying though, if I have misinformation that I think is fact, PLEASE correct me, I wont be offended :gethigh:

Ive decided to skip on using the PF cake method for numerous reasons, mostly for money, ease (IMO), and yield. Im on a limited budget, so I decided to use liquid cultures to cultivate the mycelium and then inoculate rye grain jars using that. Since I cant afford to keep paying for spores, the fact that liquid cultures produce so much so easily and quickly is great for me.

I searched the forums but havent found anything solid, so if its there and I missed it, I apologize in advance. First of all, Im new to this, so Ive never actually seen a jar of liquid culture fully colonized before, so Im not sure what Im looking for exactly. My camera isnt working and my phones camera sucks so Ill have to do my best to describe whats going on in the jar.

Theres big clumps of white, silky....for lack of better word...stuff in the jar. Theres a few circular shaped things as well. Theres no green or anything, so no contams as far as I can see. But Im just not sure of what the mycelium is exactly supposed to look like at all, seeing as Ive never seen it before in person. I can only seem to find pictures online of rye or cake jars but none of liquid culture jars.

Also, how will I know when its fully colonized? Its been going for a good week or so. Im working on a magnetic stirrer, but for now Ive just been shaking the jars in a circle and creating a vortex at least once a day. Not sure if this helps anything but thats what Im here for. And once its colonized, how should I go about innoculating a rye grain jar?

Thanks in advance for your time!


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OfflineMHbound
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Re: First time grower using liquid culture, have a few questions [Re: TrippingBillies3]
    #8600607 - 07/06/08 12:25 PM (15 years, 7 months ago)

http://www.shroomery.org/forums/showflat.php/Number/7871322#7871322

Thats what a fully colonized one should look like. Pretty much there should be that white stringy shit you are talking about all throughout the jar. The round things...If they are in the LC then its probably balls of spores if you are talking about round circular things on the side of the jars its mycelium forming on the sides. Just shake them and it will keep everything broken up or should.


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OfflineTrippingBillies3
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Re: First time grower using liquid culture, have a few questions [Re: MHbound]
    #8600615 - 07/06/08 12:29 PM (15 years, 7 months ago)

Quote:

MHbound said:
http://www.shroomery.org/forums/showflat.php/Number/7871322#7871322

Thats what a fully colonized one should look like. Pretty much there should be that white stringy shit you are talking about all throughout the jar. The round things...If they are in the LC then its probably balls of spores if you are talking about round circular things on the side of the jars its mycelium forming on the sides. Just shake them and it will keep everything broken up or should.


Great! That's exactly what my jar looks like. And for the round things, it's not on the side but it's actually just floating around, so I'm guessing it's a ball of spores?


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InvisibleDJYoshaBYD
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Registered: 04/07/07
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Re: First time grower using liquid culture, have a few questions [Re: MHbound]
    #8600623 - 07/06/08 12:32 PM (15 years, 7 months ago)

well, if you are getting mushies from your grow, then why arent you just making your own spore syringes? Its alot easier to do than LC, and LC is a pain in the ass.. What about having a master jar of colonized grain.. Then just always keep and extra on hand.. you will always have fungus, and a grain to grain transfer grows quickly.. Especially if you are growing on grain..

again, you dont have to buy spore syringes if you get mushrooms, because they produce spores.. And the cool thing about having colonized grain on hand, is the same jar can inoculate HEEELLLLAAAA jars.. each kernel is an inoculation point...

just a suggestion, because there are alot of easier, more fruitful ways to produce mycelium for inoculating...


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InvisibleDJYoshaBYD
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Re: First time grower using liquid culture, have a few questions [Re: DJYoshaBYD]
    #8600624 - 07/06/08 12:33 PM (15 years, 7 months ago)

and it wouldnt be a ball of spores growing.. it would be a ball of mycelium growing.. Mycelium grows from spores


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OfflineTrippingBillies3
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Re: First time grower using liquid culture, have a few questions [Re: DJYoshaBYD]
    #8600658 - 07/06/08 12:41 PM (15 years, 7 months ago)

Quote:

DJYoshaBYD said:
well, if you are getting mushies from your grow, then why arent you just making your own spore syringes? Its alot easier to do than LC, and LC is a pain in the ass.. What about having a master jar of colonized grain.. Then just always keep and extra on hand.. you will always have fungus, and a grain to grain transfer grows quickly.. Especially if you are growing on grain..

again, you dont have to buy spore syringes if you get mushrooms, because they produce spores.. And the cool thing about having colonized grain on hand, is the same jar can inoculate HEEELLLLAAAA jars.. each kernel is an inoculation point...

just a suggestion, because there are alot of easier, more fruitful ways to produce mycelium for inoculating...


Sounds like a good idea. Maybe I'll inoculate the master jar with this jar I have going now? To do that would I just pour a little of the colonized liquid culture into the jar?


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OfflineMHbound
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Re: First time grower using liquid culture, have a few questions [Re: TrippingBillies3]
    #8600676 - 07/06/08 12:46 PM (15 years, 7 months ago)

Nooo. You should have holes to draw up the liquid into a STERILE syringe. Once you open that jar lid you expose it to all of the nasty shit on the outside. You flame the needle tip until red hot, draw up whatever amount of liquid you are using, and then inoculate your jars being sure to flame the needle tip between each jar. Shake the jar up before you draw up your liquid sometimes there can be chunks of mycelium which can block you from being able to draw up the LC. Using grain to grain you can easily inoculate 10 jars with one other jar. The good thing about it is that it is already colonized mycelium so that means you don't have to wait for the spores to germinate saves you around a week.


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InvisibleDJYoshaBYD
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Re: First time grower using liquid culture, have a few questions [Re: MHbound]
    #8600688 - 07/06/08 12:51 PM (15 years, 7 months ago)

^^^^ spoken true, my friend...

but yeah.. grain 2 grain is my absolute favorite, and since it is already colonized, I usually just get some tweezers, take some kernels out, and just drop them in the other jar.. they grow so fast, it really doesnt give anything else any time to set in.. live fungus will start growing again withing 24 hours, and pick up speed very quickly...


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OfflineTrippingBillies3
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Re: First time grower using liquid culture, have a few questions [Re: MHbound]
    #8600701 - 07/06/08 12:54 PM (15 years, 7 months ago)

Good stuff. This definitely seems a lot more practical. I hate asking all these damn questions but to PC a syringe should I just stick it in there or wrap it in something (no pun intended)?


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OfflineMHbound
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Re: First time grower using liquid culture, have a few questions [Re: TrippingBillies3]
    #8600730 - 07/06/08 01:02 PM (15 years, 7 months ago)

I fill mine with javex, let them sit for a bit. purge them out, refill with clean water a few times to rinse bleach out of them.. then fill with bottle water and wrap in foil, pc them along with a batch of jars, and bingo. I got that from someone, and thats what I do. Then since you'll be using your LC you would just purge out the sterile water in the syringe, and immediately move on to draw up your LC. Being sure to flame the needle first, and flame again before you put the cap on or before you inoculate any jars.


--------------------


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OfflineTrippingBillies3
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Re: First time grower using liquid culture, have a few questions [Re: MHbound]
    #8600740 - 07/06/08 01:07 PM (15 years, 7 months ago)

Awesome, I think that's all I need. Thanks for all the info!


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Offlinemattyshire
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Re: First time grower using liquid culture, have a few questions [Re: MHbound]
    #26716302 - 06/03/20 09:45 AM (3 years, 8 months ago)

i used aalmost a whole syringe of liquid culture into one grain bag. is this bad? or is it maybe good? will they be more potant, or will they even grow?


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Offlinemarvins
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Re: First time grower using liquid culture, have a few questions [Re: mattyshire]
    #26716317 - 06/03/20 09:51 AM (3 years, 8 months ago)

Quote:

mattyshire said:
i used aalmost a whole syringe of liquid culture into one grain bag. is this bad? or is it maybe good? will they be more potant, or will they even grow?




https://www.shroomery.org/forums/showflat.php/Number/26022486

I couldnt find it right now but thats a great thread for LC, inside there was a guy who tested different qtys of LC in bags...


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Offlinemarvins
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Re: First time grower using liquid culture, have a few questions [Re: marvins]
    #26716319 - 06/03/20 09:52 AM (3 years, 8 months ago)

Btw is a great thread, i read it all and learned a lot i think.


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OfflineOldManRiver
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Re: First time grower using liquid culture, have a few questions [Re: mattyshire]
    #26716362 - 06/03/20 10:13 AM (3 years, 8 months ago)

Quote:

mattyshire said:
i used aalmost a whole syringe of liquid culture into one grain bag. is this bad? or is it maybe good? will they be more potant, or will they even grow?




More solution doesn't accelerate anything.  Really you need just a little bit.  It certainly doesn't change strength.


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OfflineOldManRiver
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Re: First time grower using liquid culture, have a few questions [Re: DJYoshaBYD]
    #26863645 - 08/04/20 11:43 PM (3 years, 6 months ago)

Quote:

DJYoshaBYD said:
well, if you are getting mushies from your grow, then why arent you just making your own spore syringes?




Because you can grow a mono culture LC, and get superior, repeatable results. LC is way better for inoculation (faster, more reliable starts), and if you have a useful isolate, more productive.


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OfflineOldManRiver
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Re: First time grower using liquid culture, have a few questions [Re: mattyshire]
    #26863653 - 08/04/20 11:46 PM (3 years, 6 months ago)

Quote:

mattyshire said:
i used aalmost a whole syringe of liquid culture into one grain bag. is this bad? or is it maybe good? will they be more potant, or will they even grow?



Simply inefficient.  A cc of LC is all you need in most cases. More doesn't do much to help, it certainly doesn't change potency.  You are squirting a bit of a living organism into food. That organism can multiply, like mold on bread, very quickly if conditions are right.  More bread spores don't spoil the loaf much faster.


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