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OfflineBmShroom
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What type of contam is this in petri?
    #26863482 - 08/04/20 09:06 PM (3 years, 5 months ago)

Hey guys, I've been getting quite a bit of this contam. What kind is it?



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Offlinerido
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Re: What type of contam is this in petri? [Re: BmShroom]
    #26863487 - 08/04/20 09:10 PM (3 years, 5 months ago)

That agar almost looks like it's not dissolved. Can you describe your agar process?


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OfflineBmShroom
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Re: What type of contam is this in petri? [Re: rido]
    #26865784 - 08/06/20 03:36 AM (3 years, 5 months ago)

Quote:

rido said:
That agar almost looks like it's not dissolved. Can you describe your agar process?




10g ELME, 10g agar, 1g yeast - 500 ml of grain water. Dissolve, PC for 20 min., According to a link from Bods tek page. I had some flakes from grain water settle on the bottom of my agar bottle. May be that's what you're seeing.
This here is clearly a contam, you can see how it stalled myc growth. But what kind is it?


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InvisiblebodhisattaMDiscordReddit
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Re: What type of contam is this in petri? [Re: BmShroom] * 1
    #26865823 - 08/06/20 05:11 AM (3 years, 5 months ago)

Mold.


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OfflineItsaMeShroomio
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Re: What type of contam is this in petri? [Re: BmShroom]
    #26865947 - 08/06/20 07:51 AM (3 years, 5 months ago)

Quote:

BmShroom said:
Quote:

rido said:
That agar almost looks like it's not dissolved. Can you describe your agar process?




10g ELME, 10g agar, 1g yeast - 500 ml of grain water. Dissolve, PC for 20 min., According to a link from Bods tek page. I had some flakes from grain water settle on the bottom of my agar bottle. May be that's what you're seeing.
This here is clearly a contam, you can see how it stalled myc growth. But what kind is it?




That seems like too rich an agar to me. Grain water alone is enough. ELME alone is enough. The two together along with yeast is too much. I think maybe you misread the tek and it was suggesting multiple separate options rather than suggesting 2% ELME *and* grain water.

Personally I skip the yeast, it seems to make growth more tomentose at best and more contam-prone at worst.


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OfflineBmShroom
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Re: What type of contam is this in petri? [Re: bodhisatta]
    #26875185 - 08/11/20 02:06 PM (3 years, 5 months ago)

Quote:

bodhisatta said:
Mold.




Thank you Bod for blessing my post :heart:


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OfflineBmShroom
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Re: What type of contam is this in petri? [Re: ItsaMeShroomio]
    #26875192 - 08/11/20 02:08 PM (3 years, 5 months ago)

Quote:

ItsaMeShroomio said:
Quote:

BmShroom said:
Quote:

rido said:
That agar almost looks like it's not dissolved. Can you describe your agar process?




10g ELME, 10g agar, 1g yeast - 500 ml of grain water. Dissolve, PC for 20 min., According to a link from Bods tek page. I had some flakes from grain water settle on the bottom of my agar bottle. May be that's what you're seeing.
This here is clearly a contam, you can see how it stalled myc growth. But what kind is it?




That seems like too rich an agar to me. Grain water alone is enough. ELME alone is enough. The two together along with yeast is too much. I think maybe you misread the tek and it was suggesting multiple separate options rather than suggesting 2% ELME *and* grain water.

Personally I skip the yeast, it seems to make growth more tomentose at best and more contam-prone at worst.




Alright I'll skip the yeast, was wondering about it myself. I actually diluted grain water by 50%. But to your point - I was seeing better results with less rich agar.


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InvisibleJosex
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Re: What type of contam is this in petri? [Re: BmShroom]
    #26875216 - 08/11/20 02:25 PM (3 years, 5 months ago)

Quote:

BmShroom said:
Quote:

bodhisatta said:
Mold.




Thank you Bod for blessing my post :heart:



:pope:


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