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OfflineYumyumyumyum
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Registered: 12/21/19
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Last seen: 5 months, 17 days
Using excess spore syringe matter on agar success rate?
    #26862755 - 08/04/20 02:27 PM (3 years, 6 months ago)

I am restarting everything since, I am at a point that the returns do not equal a fresh start using everything I have learned over the last 9 months. I was able to keep one syringe worth of inoculation going before all my prints, agar, and jars are not in good enough shape. I am going to try a three pack variety B+, GT and Mexican Dutch kings.

The question
I am going to do .33 per jar, 8 jars. I will have 3.3cc left of one of those strains and was wondering what the viability of agar colonization is? Certain things seem to act differently. Should I scribble it? hit it in the middle? any type of pattern? Or should I just go .50 on each jar? (Personally, I think that was part of the problem with my current project. too much moisture in the jar). Yeah, yeah I know a good grower can keep one thing going forever. I am just at the point at diminished returns and it would be nice to apply everything evenly that I have learned instead of 9 months of piece meal.

One other question, in this weather its ok to just store jars in a dark place nothing else needed? I was using heated water bins across the board but the ambient temperature is now higher and the added humidity of heated evaporation was not doing my project good.

The End disaster, the good times and what it started from 9 months ago.


Edited by Yumyumyumyum (08/04/20 02:36 PM)


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OfflineRapjack
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Registered: 05/15/17
Posts: 483
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Last seen: 2 years, 4 months
Re: Using excess spore syringe matter on agar success rate? [Re: Yumyumyumyum]
    #26862849 - 08/04/20 03:24 PM (3 years, 6 months ago)

Spores to agar is the best way to get clean spawn. People recommend putting a drop in one of the "corners" and streaking it in a zig zag with a flamed needle or preferably an inoculation loop. It'll stretch the spores out and give you maximum surface area of colonized agar to find a good transfer.

You can keep one culture going forever, with agar.  :smile:

Darkness and heat for jars is outdated info and possibly counterproductive. Room temp on a shelf with 12/12 or ambient light is standard. Remember that the inside of the jars are always warmer than the outside, if they go above the low 80's it starts to favor competitor organisms over myc.



--------------------


Edited by Rapjack (08/04/20 03:31 PM)


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OfflineYumyumyumyum
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Registered: 12/21/19
Posts: 412
Loc: PacNW
Last seen: 5 months, 17 days
Re: Using excess spore syringe matter on agar success rate? [Re: Rapjack]
    #26862910 - 08/04/20 03:56 PM (3 years, 6 months ago)

Thanks for the reply, I was figuring as much but might as well ask because sometimes the conventional wisdom is incorrect. All my dishes are about spent or contaminated. My prints are producing dense white material but it does not seem like mushrooms(no tendrils). The summer weather really started to wreck things in July. That is what, put the jars in dark room that I have my FC in. I did not know about the light for colonization(the room has a 12/12 but I keep the jars in a cabinet). I am using grow lights I am not comfortable keeping stuff in my window. I have a garden dump patch that gets sun all day long but its not doing anything other than looking like an ugly dirt patch.


Edited by Yumyumyumyum (08/04/20 04:01 PM)


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