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OfflineTerski
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Registered: 06/19/20
Posts: 23
Last seen: 2 years, 6 months
First time with Agar, help with spotting contam?
    #26862798 - 08/04/20 02:56 PM (3 years, 6 months ago)

Hey guys this is my first time attempting to any step for cultivation so I'm on the road of learning and after studying decided to start off with AGAR. I Inoculated using a spore syringe. It has been 8 days and am wondering if some of you with knowledge and experience could help me determine if things are going okay or if I need to start from scratch? Also any tips on if I should start making my first strangers yet, etc. Thank you in advance, thanks for checking out my first post to shroomery, and to many more 🙂.


Edited by Terski (08/04/20 04:24 PM)


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OfflineRapjack
Oat Soakin' Toker
I'm a teapot

Registered: 05/15/17
Posts: 483
Loc: Elsewhere
Last seen: 2 years, 4 months
Re: First time with Agar, help with spotting contam? [Re: Terski]
    #26862831 - 08/04/20 03:15 PM (3 years, 6 months ago)

#1 and #2 look like mold but the rest of them look ok from what I can tell. If you posted more focused pics of them with a strong light shining through it'll help us see harder to spot contams easily. The white parts all look like myc and if there's no bacterial "film" growing underneath it I'd say that's a good place to transfer from. The more organized / symmetrical the better.

Good job!

I'll also check back later and see if you posted more pics.

PS: Please don't tell us where the syringe came from. Those spores are only for looking at under microscopes.


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Edited by Rapjack (08/04/20 03:38 PM)


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OfflineTerski
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Registered: 06/19/20
Posts: 23
Last seen: 2 years, 6 months
Re: First time with Agar, help with spotting contam? [Re: Rapjack]
    #26862900 - 08/04/20 03:52 PM (3 years, 6 months ago)

Thanks man I'm just getting started and already having fun with all this I think the big thing is just patience and not overthinking things which I am great at haha here's a couple more with light shining through, I suspect this first one has a little mold growing up around the top edge of our can see beyond my writing? Didn't know if you meant light shing onto the plates or through the plates so did a couple of each also sorry about the condensation I now know to pour my agar at a slightly lower temp than I tried this time.



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OfflineRapjack
Oat Soakin' Toker
I'm a teapot

Registered: 05/15/17
Posts: 483
Loc: Elsewhere
Last seen: 2 years, 4 months
Re: First time with Agar, help with spotting contam? [Re: Terski]
    #26863600 - 08/04/20 11:17 PM (3 years, 6 months ago)

Definitely mold in the first pic. It looks like it might have snuck in through that crack in the parafilm. The others look good from what I can tell. Look forward to seeing you progress and welcome to the Shroomery.


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Edited by Rapjack (08/04/20 11:17 PM)


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