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OfflineBmShroom
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Tub - No pins in a month, but all white
    #26862528 - 08/04/20 11:54 AM (3 years, 5 months ago)

Hey guys, this is my second attempt at UnBodified tub grow. First one ever did great, even though I knew less than what I do now. First pins in 2 weeks after mixing oat grains with coir substrate. The surface didn't even look fully colonized. I'm attaching a few pics of that first tub.






That was a few months ago. Now I'm trying again. So whats confusing is why this new tub (that I think looks way better than the first) is not pinning yet?
The lids were flipped as per Bod's tek, both times. I've just set the lid up on the blue latches to give it more FAE, thinking it may trigger pinning. That was a couple of days ago. For about 3 and a half weeks there was no fan. The sides of tub were covered in moisture that whole time. There is now an indirect fan blowing in the room. There are still some beads of moisture left on the surface. Before the fan - the whole surface was covered in small droplets, without me even misting it. Sub temp is steady 75F.
I'm just being a bit paranoid of not seeing fruits yet. Can you take a look at let me know if the surface looks good? White is turning yellowish.
Also when someone says the tub "should be fully colonized" - do they mean the whole substrate should be white if I look at the tub from the side OR do they mean that the SURFACE must be fully white?









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OfflineRapjack
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Re: Tub - No pins in a month, but all white [Re: BmShroom]
    #26862535 - 08/04/20 11:58 AM (3 years, 5 months ago)

It stalled because it's dried out. The surface needs a RH of about 95% to produce pins, give it a light mist every time it dries out again and you might be able to get some fruits still. You want a fine mist to be covering the surface at all times.

The yellowish color is what happens when myc gets damaged by heat or dryness.


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Edited by Rapjack (08/04/20 12:00 PM)


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OfflineBmShroom
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Re: Tub - No pins in a month, but all white [Re: Rapjack]
    #26862561 - 08/04/20 12:11 PM (3 years, 5 months ago)

Dang it! Yea I had to be away for 2 weeks and someone was supposed to mist it....


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OfflineBmShroom
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Re: Tub - No pins in a month, but all white [Re: Rapjack]
    #26862565 - 08/04/20 12:13 PM (3 years, 5 months ago)

Besides that yellow color - would you say that colonization of the surface is good?


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InvisibleRoger Clemency
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Re: Tub - No pins in a month, but all white [Re: BmShroom]
    #26862673 - 08/04/20 01:21 PM (3 years, 5 months ago)

Both tubs look very bacterial to me, and the second looks dry as well.

You can see on the first tub how the myc never really moved gracefully off the grains and onto all the bulk sub. The second tub looks very unhappy and maybe a healthy tub could get that look from serious drying but the side view also shows myc not really coming off the grains well.

How did you inoculate your spawn?


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OfflineBmShroom
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Re: Tub - No pins in a month, but all white [Re: Roger Clemency]
    #26862698 - 08/04/20 01:42 PM (3 years, 5 months ago)

Used agar petri dishes to grow out what looked to be healthy myc. Transferred 3 times for it to look good. I do my work in SAB. All was white and rhizomorphic, did not see any other colors. Then used the whole 60mm agar round per jar.
Funny you say bacterial because I have 10 jars with myc that looks all puffy like a cloud and myc just wont move down to the bottom of the jar for 2 weeks now. As I understand fluffy myc is a sign of bacteria?




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OfflineA.k.aM
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Re: Tub - No pins in a month, but all white [Re: BmShroom]
    #26862729 - 08/04/20 02:07 PM (3 years, 5 months ago)

That first one needs way more air too. I’m surprised it fruited at all really based on how little it colonized.


Those jars are definitely contaminated too, how do the plates look?


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OfflineBmShroom
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Re: Tub - No pins in a month, but all white [Re: A.k.a]
    #26862734 - 08/04/20 02:11 PM (3 years, 5 months ago)

here is my post I just made about the petris

https://www.shroomery.org/forums/showflat.php/Number/26862727


Edit - unrelated


Edited by BmShroom (08/04/20 02:39 PM)


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Offlinegabbk
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Re: Tub - No pins in a month, but all white [Re: BmShroom]
    #26862735 - 08/04/20 02:12 PM (3 years, 5 months ago)

Yes, it is pretty bacterial to me.

The yellow tone is a clear sign of bacterial issue. The mycelium is producing metabolites (yellow liquid) to fight it.
It will fruit eventually if you mantain it's humidity correctly, but the yields and speed growth will be much lower compared to a healthy mycelium.
I've had it happen to me the very first grow (with agar and SAB also). But I couldn't recognize visually bacterial contam yet at that time. And it happened to me because of my poor sterile technique at that time. When that happened to me, my jars took 6 weeks to colonize and 31 days to pin, when normally it should be around 2-4 weeks and 1-3 weeks respectively.

Recognizing fungal contamination is pretty easy. It is generally pink, black, or blue. Don't get me wrong, some types of bacteria could also manifest with colors and various forms too, but the type of bacteria that I'm referring to (which I also think you are going through) is the one that goes undercover because it "hitch-hikes" onto the mycelium.
Personally, when my agars get this type of bacteria I can see a halo around the edge as shown in this picture:


You also can recognize that those jars are not 100% clean. It should look more like cottonish instead of a liquid yoghurt so to speak. More similar like this, to give an example:


Another indicator is, when you break the grains apart it should break easily within a few shakes. If you have to make a big effort, that's a big sign of not a good spawn.

Oh! I almost forget another one more indicator, the smell! If it smells fruity or acidic, it's a great sign of bacteria!

Hope it helps!


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Edited by gabbk (08/04/20 02:24 PM)


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InvisibleSaul Ptamets
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Re: Tub - No pins in a month, but all white [Re: gabbk]
    #26862747 - 08/04/20 02:18 PM (3 years, 5 months ago)


idk if anyone can see but do any of these look contaminated????


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Offlinesmalltalk_canceled
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Re: Tub - No pins in a month, but all white [Re: Saul Ptamets]
    #26862749 - 08/04/20 02:22 PM (3 years, 5 months ago)

Dear brother, you must zoom in on every jar and preferably on the spots you are worried about. Not even Shroom Tzu can see contams at that distance


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OfflineBmShroom
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Re: Tub - No pins in a month, but all white [Re: gabbk]
    #26862753 - 08/04/20 02:24 PM (3 years, 5 months ago)


Quote:

gabbk said:
Yes, it is pretty bacterial to me.

The yellow tone is a clear sign of bacterial issue. The mycelium is producing metabolites (yellow liquid) to fight it.
It will fruit eventually if you mantain it's humidity correctly, but the yields and speed growth will be much lower compared to a healthy mycelium.
I've had it happen to me the very first grow (with agar and SAB also). But I couldn't recognize visually bacterial contam yet at that time. And it happened to me because of my poor sterile technique at that time. When that happened to me, my jars took 6 weeks to colonize and 31 days to pin, when normally it should be around 2-4 weeks and 1-3 weeks respectively.

Recognizing fungal contamination is pretty easy. It is generally pink, black, or blue. Don't get me wrong, some types of bacteria could also manifest with colors and various forms too, but the type of bacteria that I'm referring to (which I also think you are going through) is the one that goes undercover because it "hitch-hikes" onto the mycelium.
Personally, when my agars get this type of bacteria I can see a halo around the edge as shown in this picture:


You also can recognize that those jars are not 100% clean. It should look more like cottonish instead of a liquid yoghurt so to speak. More similar like this, to give an example:


Another indicator is, when you break the grains apart it should break easily within a few shakes. If you have to make a big effort, that's a big sign of not a good spawn.

Oh! I almost forget another one more indicator, the smell! If it smells fruity or acidic, it's a great sign of bacteria!

Hope it helps!




Thank you for your input! So have this jar that I'm not sure if contaminated or not. I was expecting more aggressive myc growth after 2 weeks left alone. Was going to use it for G2G. It looks similar to yours, but what do you think?



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OfflineBmShroom
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Re: Tub - No pins in a month, but all white [Re: BmShroom]
    #26862764 - 08/04/20 02:37 PM (3 years, 5 months ago)

Quote:

BmShroom said:
here is my post I just made about the petris

https://www.shroomery.org/forums/showflat.php/Number/26862727




My bad, that post is unrelated to my current issue. Since its a new run of plates. Here is what one of the plates looked like for the jars that went into this tub:


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Offlinegabbk
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Re: Tub - No pins in a month, but all white [Re: BmShroom]
    #26862780 - 08/04/20 02:43 PM (3 years, 5 months ago)

That jar looks good to my eye! You could go ahead and spawn it, but I would suggest not to G2G with it. There's something at the upper part of the jar that stranges me a little, maybe those branches on the glass (but I dunno exactly about that, I've never had it happen to me; I would wait for someone to take a look ok
n this). And taking account what you are telling, the jar shouldn't stall or get slower when being close to finish colonizing the spawn, instead it should be faster (the more mycelium, the more faster it takes over). So my best advice would be to wait to get better at sterile technique to do a proper G2G. I've done G2G also when I began, to save time ahead, but did it with my mentioned bacterial jar and expanded that shit through all ten jars :eek:. When I realised, I immediately wished there was a chance to go back in time for not doing that :lol:

Edit: I've just seen that Petri. It looks super clean and awesome!


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Edited by gabbk (08/04/20 03:02 PM)


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OfflineBmShroom
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Re: Tub - No pins in a month, but all white [Re: gabbk]
    #26862795 - 08/04/20 02:55 PM (3 years, 5 months ago)

Quote:



Edit: I've just seen that Petri. It looks super clean and awesome!




OK, not going to G2G, may be that was my issue to begin with. I forgot that yes I did G2G for this new tub.

:smile:  Could you look at my post about plates? I'm not sure about them. https://www.shroomery.org/forums/showflat.php/Number/26862727


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InvisibleBlazer420
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Re: Tub - No pins in a month, but all white [Re: Saul Ptamets]
    #26862808 - 08/04/20 03:03 PM (3 years, 5 months ago)

Quote:

Saul Ptamets said:

idk if anyone can see but do any of these look contaminated????




Looking rather wet.


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Offlinegabbk
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Re: Tub - No pins in a month, but all white [Re: BmShroom]
    #26862828 - 08/04/20 03:14 PM (3 years, 5 months ago)

Ohh so you did the same as I did hahaha. Well, at the end of the day the most important thing on this hobby is learning!

I took a look on those plates. They seem REALLY weird. Very cobwebly like:shocked:
That seems very different to a regular normal tormentose growth. To be honest I would ditch these plates and aim for something like the last agar you posted on here. That's a pretty neat agar.
You will also recognize a proper tormentose in some plates (not that agar on the other post). It could even occur when transferring the same mycelium to another plate; it could switch from rhyzo to tormentose but you don't have to worry about it. It doesn't tell anything. I've had some tormentose mycelium s that grew as fast as rhyzos


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Re: Tub - No pins in a month, but all white [Re: Blazer420]
    #26863400 - 08/04/20 08:12 PM (3 years, 5 months ago)




does the bag look fine and in the jars is it the verm that's yellow or is that contam


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OfflineBmShroom
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Re: Tub - No pins in a month, but all white [Re: Saul Ptamets]
    #26943720 - 09/19/20 08:43 AM (3 years, 4 months ago)

Quote:

Saul Ptamets said:



does the bag look fine and in the jars is it the verm that's yellow or is that contam




Hey Saul, did you make a new thread about your question? How did those turn out?


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InvisibleSaul Ptamets
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Re: Tub - No pins in a month, but all white [Re: BmShroom]
    #26944150 - 09/19/20 01:12 PM (3 years, 4 months ago)

the cakes werent contaminated but still, fuck cakes they're retarded, never doing them again


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