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OfflineBloob
Biochem Student

Registered: 09/25/19
Posts: 16
Last seen: 1 month, 21 days
Agar Plates and Liquid Culture problems
    #26862512 - 08/04/20 11:40 AM (3 years, 5 months ago)

Hi!

Ok so lets start with the agar plates, first time i try them.
So they have been inoculated over a week ago and shows no sign of mycelium nor contamination.
I am thinking I might have sprayed a bit to much with the syringe adding to much water to it, but it doesn't look wet at this moment.
Other thing that could be the problem is that the recipe I follow says to use Potato flakes, but I could not find this in my country so I went with potato Flour instead which I assume is not too far off

Using this guide:
https://www.shroomery.org/forums/showflat.php/Number/19208976#19208976

Picture:




Next up is my Liquid cultures, this is the third time I am trying it and same thing happens every time.
First two times I used 4% honey in water.
All guides recommend to get the PC up to 15Psi then boil for 15-20 min. But when I do this i get crystallization at the bottom of the jar, it takes like 7min for my stove to even reach 15Psi and then 15 more minutes is probably to long.
So the next time i started the stove and just timed it 15min even though it just hit 15Psi at half time, still getting the crystallization at the bottom.
Both attempts show no sign of mycelium or anything after two weeks even.

So the third attempt I used malt extract instead since I have seen more success for others with this. And maybe I wont get the crystallization, but of course same thing happens. All though this last attempt shows signs of contaminations. (White fluff on top in the picture bellow)

Guide:
https://www.shroomery.org/forums/showflat.php/Number/18381574


Picture:



Worth noting that all of these attempts have been made with syringes that I made with my own spore prints of my own spawns. All though they have worked in my BRF Jars.
Thankful for all the tips I can get :smile:


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