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OfflineCowboy Buckie
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Easiest way to extract from liquid culture jar.
    #26862044 - 08/04/20 06:28 AM (3 years, 5 months ago)

I've been having good luck with liquid culture jars except trying to get the liquid from the jars. I have professionally made lids it an injection port and fresh air port.  The problem is when I stick syringe through the injection port I can not draw do the a vacuum created. I've tried putting a cpl 16 gauge needles through the port as well but this doesn't seem to help either. I'm using 1 1/2" needles so I have to tilt the jar slightly to reach the liquid.
thnks


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Offlinetonz0Funguy
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Registered: 09/03/19
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Re: Easiest way to extract from liquid culture jar. [Re: Cowboy Buckie]
    #26862122 - 08/04/20 07:39 AM (3 years, 5 months ago)

If your purchased the correct syringe filter you should have 0 problems this is what allows air into the jar. I turn whole jar upside down when i extract lc from the jar and it shouldn't leak with the lids you speak of.


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OfflineCowboy Buckie
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Re: Easiest way to extract from liquid culture jar. [Re: tonz0Funguy]
    #26863873 - 08/05/20 06:47 AM (3 years, 5 months ago)

Quote:

tonz0Funguy said:
If your purchased the correct syringe filter you should have 0 problems this is what allows air into the jar. I turn whole jar upside down when i extract lc from the jar and it shouldn't leak with the lids you speak of.




Well this would explain a few things. Thank you. Do these filters have a name?


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Offlinetonz0Funguy
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Re: Easiest way to extract from liquid culture jar. [Re: Cowboy Buckie]
    #26863901 - 08/05/20 07:16 AM (3 years, 5 months ago)

The hydrophobic PTFE membrane with a polypropylene 25mm will work these are pc'able. Go on ebay or any vendor here will have them or you can be lazy and order pre-made I did lol I only have 2 lc lids. I prefer to go from agar these days. I like to see its clean toss on mini-cakes on a pp5 patsy plate and knock up things, plus a slurry in hurry is always fun!!! A mini-cake are great!!


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"The search bar is a oracle"


Edited by tonz0Funguy (08/05/20 07:22 AM)


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