Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
OfflineSeamuss
Bad attitude


Registered: 07/02/20
Posts: 154
Last seen: 9 months, 21 days
Can you re use a un-germinated agar dish?
    #26861280 - 08/03/20 05:32 PM (3 years, 6 months ago)

Decided to get into agar recently, I may have went over board at first haha, I germinated around 40 Petri dishes, have done some transfers, attempted to clone some wild GT(unsuccessfully), and have weeded thought a bit of contamination along the way. Some of them I admit looks pretty cool, and I can’t bring myself to throw all of them out just yet, though I have started to toss most to make room for up coming transfers. I have 15 dishes out of the 40 that I inoculated with a MSS that did not germinate and didn’t contaminate. So my question is, has anyone ever re used these ungerminated plates? Or is that just too risky for a transfer, especially my first T2s


Extras: Filter Print Post Top
Offlineskullhuman
the skullman cometh
Male User Gallery


Registered: 06/15/09
Posts: 1,473
Last seen: 9 months, 1 day
Re: Can you re use a un-germinated agar dish? [Re: Seamuss]
    #26861289 - 08/03/20 05:35 PM (3 years, 6 months ago)

Something could still take in those plates, I would just redo them so that you can be sure you're starting from 0.


--------------------


Extras: Filter Print Post Top
OfflineRapjack
Oat Soakin' Toker
I'm a teapot

Registered: 05/15/17
Posts: 483
Loc: Elsewhere
Last seen: 2 years, 4 months
Re: Can you re use a un-germinated agar dish? [Re: Seamuss]
    #26861295 - 08/03/20 05:38 PM (3 years, 6 months ago)

I wouldn't trust em. Especially when pouring more dishes is pretty cheap. If you're trying to make clean myc you want to be 100% sure your starting base is clean too. Instead, when you pour new dishes you can put three or four wedges on each one to not use as many while cleaning multiple cultures.


--------------------


Extras: Filter Print Post Top
OfflineSeamuss
Bad attitude


Registered: 07/02/20
Posts: 154
Last seen: 9 months, 21 days
Re: Can you re use a un-germinated agar dish? [Re: Rapjack]
    #26861348 - 08/03/20 06:01 PM (3 years, 6 months ago)

I have noticed in some threads where others have taken 2-3 transfers and put them on one dish and I decided to do that as well on my most recent transfers. Definitely a better way to consolidate and save some plates. Maybe I’ll try to use them for new germs if something goes bad with my transfers in the near future.


Extras: Filter Print Post Top
Offlineskullhuman
the skullman cometh
Male User Gallery


Registered: 06/15/09
Posts: 1,473
Last seen: 9 months, 1 day
Re: Can you re use a un-germinated agar dish? [Re: Seamuss]
    #26861352 - 08/03/20 06:03 PM (3 years, 6 months ago)

Why try to save plates anyway? Just make more when you need more.  Agar is cheap and so are plates.


--------------------


Extras: Filter Print Post Top
OfflineSeamuss
Bad attitude


Registered: 07/02/20
Posts: 154
Last seen: 9 months, 21 days
Re: Can you re use a un-germinated agar dish? [Re: skullhuman]
    #26861374 - 08/03/20 06:24 PM (3 years, 6 months ago)

I just feel like throwing 15 good plates away to make 20 more is a bit of a waste. I won’t waste a clean culture on one for sure but damn these are ready to go! Lol


Extras: Filter Print Post Top
OfflineRapjack
Oat Soakin' Toker
I'm a teapot

Registered: 05/15/17
Posts: 483
Loc: Elsewhere
Last seen: 2 years, 4 months
Re: Can you re use a un-germinated agar dish? [Re: skullhuman]
    #26861420 - 08/03/20 06:50 PM (3 years, 6 months ago)

Quote:

skullhuman said:
Why try to save plates anyway? Just make more when you need more.  Agar is cheap and so are plates.




I like to test a bunch of clones at once. Having three plates instead of 12 for example is huge savings when it's multiple transfers to clean them. Plates are cheap but the time to prepare more is just not worth it if I'm transferring asap anyway. Or if a culture is really iffy I'll do four wedges and only transfer from the best one. For the final ones that go to grains I'll do one wedge per, and make multiple copies.

The caveat though is you need good sterile technique.


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* When using agar dishes wouldn't it be wise too TheGreenLantern 1,665 3 04/13/02 01:35 PM
by ParticleMan
* Cool! Successful agar dishes...what now....? Frankenstein 2,446 3 04/11/02 07:55 AM
by Anno
* made agar dishes, now what? thisone 1,431 3 11/14/03 06:45 PM
by thisone
* old agar dish boomer420 1,323 8 04/14/04 07:08 PM
by thisspock
* AgaR dishes MsPacMan 1,440 4 10/07/02 09:05 PM
by MsPacMan
* Re: I need glass slides, testubes, and agar dishes Anonymous 1,830 9 08/20/00 09:25 PM
by Anonymous
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,314 38 05/11/01 11:08 AM
by MAIA
* How to make agar 2 agar transfer? esp 6,677 7 01/08/04 11:08 AM
by YidakiMan

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
107 topic views. 28 members, 135 guests and 52 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.007 seconds on 12 queries.