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Seamuss
Bad attitude


Registered: 07/02/20
Posts: 154
Last seen: 9 months, 21 days
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Can you re use a un-germinated agar dish?
#26861280 - 08/03/20 05:32 PM (3 years, 6 months ago) |
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Decided to get into agar recently, I may have went over board at first haha, I germinated around 40 Petri dishes, have done some transfers, attempted to clone some wild GT(unsuccessfully), and have weeded thought a bit of contamination along the way. Some of them I admit looks pretty cool, and I can’t bring myself to throw all of them out just yet, though I have started to toss most to make room for up coming transfers. I have 15 dishes out of the 40 that I inoculated with a MSS that did not germinate and didn’t contaminate. So my question is, has anyone ever re used these ungerminated plates? Or is that just too risky for a transfer, especially my first T2s
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skullhuman
the skullman cometh



Registered: 06/15/09
Posts: 1,473
Last seen: 9 months, 1 day
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Re: Can you re use a un-germinated agar dish? [Re: Seamuss]
#26861289 - 08/03/20 05:35 PM (3 years, 6 months ago) |
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Something could still take in those plates, I would just redo them so that you can be sure you're starting from 0.
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Rapjack
Oat Soakin' Toker


Registered: 05/15/17
Posts: 483
Loc: Elsewhere
Last seen: 2 years, 4 months
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Re: Can you re use a un-germinated agar dish? [Re: Seamuss]
#26861295 - 08/03/20 05:38 PM (3 years, 6 months ago) |
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I wouldn't trust em. Especially when pouring more dishes is pretty cheap. If you're trying to make clean myc you want to be 100% sure your starting base is clean too. Instead, when you pour new dishes you can put three or four wedges on each one to not use as many while cleaning multiple cultures.
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Seamuss
Bad attitude


Registered: 07/02/20
Posts: 154
Last seen: 9 months, 21 days
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Re: Can you re use a un-germinated agar dish? [Re: Rapjack]
#26861348 - 08/03/20 06:01 PM (3 years, 6 months ago) |
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I have noticed in some threads where others have taken 2-3 transfers and put them on one dish and I decided to do that as well on my most recent transfers. Definitely a better way to consolidate and save some plates. Maybe I’ll try to use them for new germs if something goes bad with my transfers in the near future.
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skullhuman
the skullman cometh



Registered: 06/15/09
Posts: 1,473
Last seen: 9 months, 1 day
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Re: Can you re use a un-germinated agar dish? [Re: Seamuss]
#26861352 - 08/03/20 06:03 PM (3 years, 6 months ago) |
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Why try to save plates anyway? Just make more when you need more. Agar is cheap and so are plates.
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Seamuss
Bad attitude


Registered: 07/02/20
Posts: 154
Last seen: 9 months, 21 days
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Re: Can you re use a un-germinated agar dish? [Re: skullhuman]
#26861374 - 08/03/20 06:24 PM (3 years, 6 months ago) |
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I just feel like throwing 15 good plates away to make 20 more is a bit of a waste. I won’t waste a clean culture on one for sure but damn these are ready to go! Lol
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Rapjack
Oat Soakin' Toker


Registered: 05/15/17
Posts: 483
Loc: Elsewhere
Last seen: 2 years, 4 months
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Re: Can you re use a un-germinated agar dish? [Re: skullhuman]
#26861420 - 08/03/20 06:50 PM (3 years, 6 months ago) |
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Quote:
skullhuman said: Why try to save plates anyway? Just make more when you need more. Agar is cheap and so are plates.
I like to test a bunch of clones at once. Having three plates instead of 12 for example is huge savings when it's multiple transfers to clean them. Plates are cheap but the time to prepare more is just not worth it if I'm transferring asap anyway. Or if a culture is really iffy I'll do four wedges and only transfer from the best one. For the final ones that go to grains I'll do one wedge per, and make multiple copies.
The caveat though is you need good sterile technique.
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