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OfflineJohnBravo
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Registered: 06/24/19
Posts: 21
Last seen: 3 years, 1 month
No growth on PDA agar - What's wrong?
    #26860738 - 08/03/20 12:32 PM (3 years, 5 months ago)

Hey all. I purchased a sleeve of 10 PDA agar from a well-reviewed seller on Etsy. He said the mix was 4g/L potato infusion, 20g/L dextrose, 15g/L agar.

This is my first foray into agar. Working in front of a flowhood. Did the first test by opening a few empty plates in front of the flowhood and incubated for a few days - no growth. A good thing!

However, then I used the dregs of an LC to inoculate the plate, using good sterile procedure. No growth after 8 days. I figured maybe since it was the last little bits of myc in the LC, perhaps it wasn't viable.

So I went with the original spore solutions. Again, no growth after roughly 13 or 14 days. I'm not sure what I'm doing wrong. For both inoculations, I basically just squirted a bit of LC/spore solution onto the plate and tipped it back and forth to distribute, as I read on a thread here.

Here are a few pics of what im seeing. You can see the spores (little black spots) just kind of chilling there.





Any ideas?


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OfflineRapjack
Oat Soakin' Toker
I'm a teapot

Registered: 05/15/17
Posts: 483
Loc: Elsewhere
Last seen: 2 years, 3 months
Re: No growth on PDA agar - What's wrong? [Re: JohnBravo]
    #26860759 - 08/03/20 12:38 PM (3 years, 5 months ago)

That's really bizarre. It looks like there's not even bacteria growing on them. My guess is that the dishes got mislabeled or the seller added some kind of preservative. When I was doing homemade PDA I never saw this.


Edited by Rapjack (08/03/20 12:39 PM)


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InvisibleZifozonke
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Registered: 03/24/19
Posts: 1,258
Re: No growth on PDA agar - What's wrong? [Re: Rapjack]
    #26860824 - 08/03/20 01:00 PM (3 years, 5 months ago)

Time to start doing your own
https://www.shroomery.org/forums/showflat.php/Number/4825501#4825501
Itll work out much cheaper and at least youll know whats gone into it.


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OfflineJohnBravo
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Registered: 06/24/19
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Last seen: 3 years, 1 month
Re: No growth on PDA agar - What's wrong? [Re: Zifozonke]
    #26861999 - 08/04/20 05:15 AM (3 years, 5 months ago)

Thanks for the input so far. Anyone else have any ideas?


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OfflineCapsicum Chinense
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Registered: 08/09/17
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Re: No growth on PDA agar - What's wrong? [Re: JohnBravo]
    #26862011 - 08/04/20 05:35 AM (3 years, 5 months ago)

The only test I can imagine is to make/buy another plate(s) and try the same spores again. That still wouldn't explain why the LC didn't take, though...


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OfflineRapjack
Oat Soakin' Toker
I'm a teapot

Registered: 05/15/17
Posts: 483
Loc: Elsewhere
Last seen: 2 years, 3 months
Re: No growth on PDA agar - What's wrong? [Re: Capsicum Chinense]
    #26863591 - 08/04/20 11:07 PM (3 years, 5 months ago)

If the spores are old they could be dried out, you could make a spore syringe and try it after a week. But it's odd to me that there isn't even bacteria, maybe it's an unusually clean print!


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