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Ombisha
Transmutant



Registered: 02/17/20
Posts: 667
Loc: Tartaria
Last seen: 1 year, 4 months
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Lions Mane not doing anything
#26859210 - 08/02/20 04:08 PM (3 years, 6 months ago) |
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Hello everyone,
figured i would try branching out into growing gourmet/medicinal mushrooms for fun. Ordered some spawn online - wine cap, oysters, reishi and lions mane. As soon as they arrived (about 5 days ago) , i dropped them onto agar dishes (2 dishes per spawn "jar"). All are already spreading out fine, with the exception of Pleurotus citrinopileatus (the spawn i received was bacterial...) and lions mane.
The lions mane spawn seemed fine, but it is not doing anything since i dropped it on the plates. I am using grain water agar (dont judge me), so could that be the reason its not growing? The spawn was on some sort of grain, so i cant see why that would be so...
Any ideas? Does it just take long time to grow? Does it need to be incubated in some specific way?
Thanks
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 12 days
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Re: Lions Mane not doing anything [Re: Ombisha]
#26859332 - 08/02/20 05:29 PM (3 years, 6 months ago) |
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LM should grow very quickly. Are you getting a good view into you plates? I mean no condensation or hard to see thru plastic... LM mycelium is very fast but super thin/wispy to begin with so maybe you're just not seeing it.
If the others are growing fine, I doubt it's your agar, and there's nothing weirdly specific about growing LM mycelium, so I can't think of anything else it would be.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Ombisha
Transmutant



Registered: 02/17/20
Posts: 667
Loc: Tartaria
Last seen: 1 year, 4 months
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Re: Lions Mane not doing anything [Re: Forrester]
#26860036 - 08/03/20 05:02 AM (3 years, 6 months ago) |
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I am getting a good look, took a picture. Looks like the grain may have slightly "puffed up", but refuses to spread onto the agar. Overnight appeared these drops of metabolites, but no contamination is visible on the agar, so im not sure what caused them.
Any ideas?
Also, by your profile im judging, that you should learn to swim, sir.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 12 days
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Re: Lions Mane not doing anything [Re: Ombisha]
#26861018 - 08/03/20 02:34 PM (3 years, 6 months ago) |
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My only guess is, due to the presence of metabolites on the grain there, it isn't spreading because it's contaminated, probably bacteria.
But that's just a guess. I'd take some different grains and try again on another plate. If the same happens, just try to use the spawn and then clone a fruit if you wanna save the culture.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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sendmehummus
AT Hiker


Registered: 08/03/20
Posts: 383
Loc: USA
Last seen: 2 years, 3 months
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Re: Lions Mane not doing anything [Re: Ombisha]
#26861397 - 08/03/20 06:38 PM (3 years, 6 months ago) |
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Soo a few months ago I learned a hard lesson in plate incubation when I did tramsfers of King, Golden and Pearl Oysters. I live in PA, the room temp usually stays between 60-70 degrees. Naturally the King and Pearl dishes did fantastic while the Golden crept out a little and just stopped completely. I was like wtf thought all oysters were supposed to be aggressive. But the room temp you are leaving your plates in is really important for that growth. Maybe double check what temps LM mycelium prefers? You may have already done this just giving you my two cents! Good luck!
-------------------- Be patient and keep it simple.
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