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OfflineCapMeh
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LC syringe to agar, how does my plate look? Cubensis
    #26859299 - 08/02/20 05:12 PM (3 years, 5 months ago)

Hey everyone, I’m still new to agar and was wondering if I could get some input on what to look for in a colonizing plate. The picture below is of Jedi mind fuck (cubensis) from an LC syringe from a random source. I inoculated about 14 plates and 1 quart rye jar. The jar is colonizing with no visible contam, and all the plates produced uneven thick fluffy myc. My main question is, regarding the picture below, what do the obvious separations of mycelium mean? And what about the stripe pattern? If you were to transfer from this plate using clock position where would you take it?



Any insight would be much appreciated! Thank you


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Offlinejcm4620
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: CapMeh]
    #26859311 - 08/02/20 05:20 PM (3 years, 5 months ago)

any sight at all would be appreciated lol i cant see a god famn thing in that pic


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PANAEOLUS FRUITING MADE SIMPLE



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OfflineCapMeh
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: jcm4620]
    #26859314 - 08/02/20 05:22 PM (3 years, 5 months ago)

Sorry I ran out of space haha I was mainly trying to show the sectoring. Let me delete some stuff and post more


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OfflineCapMeh
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: CapMeh]
    #26859356 - 08/02/20 05:38 PM (3 years, 5 months ago)

These no-pour dishes suck for taking pictures.. hope this helps



I noticed this one has a blue tint on the surface...?


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Offlinejcm4620
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: CapMeh]
    #26859417 - 08/02/20 06:09 PM (3 years, 5 months ago)

man i honestly cant give an opinion here i cant see anything good enough. all i can suggest is to look for growth like this if its cubensis



or if its panaeolus look for this



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PANAEOLUS FRUITING MADE SIMPLE



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OfflineCapMeh
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: jcm4620]
    #26859438 - 08/02/20 06:23 PM (3 years, 5 months ago)

Yeah I understand, can you at least see the sectoring in the first picture? Does that mean different genetics? Contam? What about the stripe pattern?


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OfflineNichrome
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: CapMeh]
    #26859516 - 08/02/20 06:51 PM (3 years, 5 months ago)

It does not look to be contaminated but that is a very diverse colony.


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OfflineCapMeh
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: Nichrome]
    #26859531 - 08/02/20 06:56 PM (3 years, 5 months ago)

Quote:

Nichrome said:
It does not look to be contaminated but that is a very diverse colony.




Okay interesting, so hopefully the separations in mycelium are just different cube genetics not connecting? I used an LC syringe and had trouble getting just one drop out, so it traveled all over the surface of the plate. Thank you!


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OfflineCapMeh
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: CapMeh]
    #26859535 - 08/02/20 06:58 PM (3 years, 5 months ago)

I’m hoping it’s just diverse tomentose growth.. but I don’t really know how this works yet.


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Offlinejcm4620
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: CapMeh]
    #26859564 - 08/02/20 07:10 PM (3 years, 5 months ago)

ya u really need better pics for me to even say one wah or another. just use the pics i posted as a ruff guide on what to look for


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OfflineCapMeh
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: jcm4620]
    #26859570 - 08/02/20 07:14 PM (3 years, 5 months ago)

I hear ya jcm, I mean I think they’re pretty clean I’m just curious what I’m looking at in the formation and division.


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OfflineCapMeh
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Re: LC syringe to agar, how does my plate look? Cubensis [Re: CapMeh]
    #26875114 - 08/11/20 01:23 PM (3 years, 5 months ago)

Okay I have some update pictures. I took transfers from two of the most uniform looking plates, and this is what they look like now.



They’re much more uniform after transferring. At this point I’m LC syringe>agar> first agar transfer. How do they look? Is it fuzzy because of tomentose mycelium? We’re the first plates chaotic looking because LC rolled around the surface of the agar cause many cubensis colonies to grow? I want to know how to tell that apart from mold contam. Any insight would be appreciated thank you.


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