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Offlineplutoniusspasticus
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Registered: 08/01/20
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Why are my petri dishes contaminating?
    #26859017 - 08/02/20 02:06 PM (3 years, 5 months ago)

Hi everyone,

I'm a noob but I think I've got a reasonably sterile technique where I can make clean plates and do clean transfers. However whenever I inoculate with my ms syringe, it comes out all slimy. Have I somehow contaminated my syringe? I'm not sure what I'm doing wrong. Any ideas would be appreciated.
Here's an example:



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InvisibleRoger Clemency
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Registered: 03/23/20
Posts: 2,005
Re: Why are my petri dishes contaminating? [Re: plutoniusspasticus]
    #26859035 - 08/02/20 02:13 PM (3 years, 5 months ago)

A lot of syringes come out like that on agar. Since spore prints aren’t totally sterile some bacteria can get in and then I guess it germinates because wherever the spore solution touches you get slimy bacteria.

I try to use prints only unless there’s something specific I want and I can only find a syringe.

You can try something called a Hot pour or sterilize a half pint of grain, germinate your spores in there, then take a single grain back to agar to clean it up.


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