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shroomshine
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second opinion on LC test (agar pour plate)
#26857597 - 08/01/20 05:41 PM (3 years, 5 months ago) |
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someone on here told me its good to go but I have my serious doubts!!
I would love to get a second opinion!
so the big issue I am having is white/off grey mold that I had a very hard time distinguishing from mycelium, so I made some bad Xfers and potentially knocked the LC up with moldy myc as well. To test my LC I made a pour plate.
this is my LC as of today, I inoculated it via "poke" method on 07/09:
 there are particles in there because I didn't clean up the glass after I crushed it (yea I know) it had those in right after it came out off the PC
this is the pour plate after 5 days:
   
and this is the pour plate today (7 days):
  I can see a lot of fluffy stuff and the patch that is/was clearly mycelium kinda stalled out.. Idk if the mycelium is just growing all over the place now or if its mold again... it does not smell like mushrooms 
thanks guys!!
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Capsicum Chinense
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Re: second opinion on LC test (agar pour plate) [Re: shroomshine]
#26857602 - 08/01/20 05:42 PM (3 years, 5 months ago) |
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Totally untrained eyes; looks like mold to me.
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sandman420
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It never did find its way into a organized growth did it. Poor lil guy.
It looked like it might at 5 days but went bananas in the next few days.
Doesnt look good.
Edited by sandman420 (08/01/20 06:07 PM)
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shroomshine
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Re: second opinion on LC test (agar pour plate) [Re: sandman420]
#26857762 - 08/01/20 07:55 PM (3 years, 5 months ago) |
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Quote:
sandman420 said: It never did find its way into a organized growth did it. Poor lil guy.
It looked like it might at 5 days but went bananas in the next few days.
Doesnt look good.
I am glad you edited your post because I have to say that giving wrong advise is one thing but coming back and being all cute about it is different. I read here a lot and its not the first time that I noticed you giving advise on a topic where it seams you don't really know what's up. Don't get me wrong, nobody is infallible but being a punk about it is unnecessary.
Peace
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sandman420
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Re: second opinion on LC test (agar pour plate) [Re: shroomshine]
#26858289 - 08/02/20 07:32 AM (3 years, 5 months ago) |
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Not sure what you are on about
I said it was looking "about like what my lc on agar looks like" on your other post at 5 days but I never saw what a shit show it was at 7 days. You wanna call out my bad advice is fine but at least call it out instead of being vague. LC always looks odd at first on agar IMO.
It certainly didnt look shit enough on day 5 for me to say for sure contam, since LC always starts funny. So not sure what the hell you want.
I'm sorry your LC is hot garbage dont take it out on me. I edit nearly all of my posts to organize my thoughts and better reflect my original intentions.
And since you said I dont know whats up, this is sitting in my shelf right now

whats sitting on yours?
I do mybest here to help people because it makes me feel good. I don't need some backlash from the person I'm trying to assist like I'm some idiot that doesn't know anything. I've been doing this for nearly 20 years I know wahts up.
In the future I will maybe have to add "to the best of my experience and what I can see" so the little puppy I throw a bone to doesnt snap my finger.
Not gonna have some NOBODY come in here and slander me. I cant be right all the time and I genuinely tried to help you now I dont much care for you.
Edited by sandman420 (08/02/20 08:32 AM)
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ManifoldPrime
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Re: second opinion on LC test (agar pour plate) [Re: sandman420]
#26858421 - 08/02/20 08:50 AM (3 years, 5 months ago) |
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Looks like mold OP.
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Nichrome
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Re: second opinion on LC test (agar pour plate) [Re: ManifoldPrime]
#26858427 - 08/02/20 08:57 AM (3 years, 5 months ago) |
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Photos are always tough to decipher. To me that looks like standard cubensis mycelium. Personally I don't see any mold and I test several LC's per week and spend hours every day with my face glued to a microscope. Looks good to me albeit tomentose and wacky growth patterns. Personally I like the weird stuff. The only way to know for sure is to do a transfer and see how it acts as a radial colony Or put it on grain and try to fruit it out. I suspect if you transfer a piece it will behave in a more standard way like people want it to.
Edited by Nichrome (08/02/20 09:06 AM)
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sandman420
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Re: second opinion on LC test (agar pour plate) [Re: Nichrome]
#26858435 - 08/02/20 09:01 AM (3 years, 5 months ago) |
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careful she may say you dont know anything if anything goes slightly wrong.
Edited by sandman420 (08/02/20 09:05 AM)
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Nichrome
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Re: second opinion on LC test (agar pour plate) [Re: sandman420]
#26858443 - 08/02/20 09:05 AM (3 years, 5 months ago) |
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Quote:
sandman420 said: I wouldn't attempt to help her she may say you dont know anything if anything goes slightly wrong.
I really don't care or have time to get butt hurt. Comments like this are no help to anyone and seem spiteful. Where does the rip in the fabric stop?
This post is about LC test plates and nothing else. Please stay on topic.
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sandman420
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Re: second opinion on LC test (agar pour plate) [Re: Nichrome]
#26858446 - 08/02/20 09:06 AM (3 years, 5 months ago) |
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you right but excuse me for being upset at being called out like your some angel that never got upset. I have a lot of time.
Forgot that mushcult must stay on topic.
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shroomshine
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Re: second opinion on LC test (agar pour plate) [Re: sandman420]
#26858528 - 08/02/20 09:52 AM (3 years, 5 months ago) |
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Thanks everybody for the opinions! I am leaning more towards mold but still going to knock a jar up to make sure...
Oh and "your ego is not your amigo" (never)
See ya!
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Re: second opinion on LC test (agar pour plate) [Re: shroomshine]
#26858674 - 08/02/20 11:30 AM (3 years, 5 months ago) |
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To me, this is myc. All of the plate.
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mushboy
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Re: second opinion on LC test (agar pour plate) [Re: shroomshine]
#26858695 - 08/02/20 11:37 AM (3 years, 5 months ago) |
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Quote:
shroomshine said: Pour
Pouring for an agar test can be tricky. Either you slop the lc everywhere or the growth is too erratic to determine. I recommend getting extra long inoc loops or long sterile swabs. Tilt your lc jar and quickly dip the loop/swab into the broth and then swipe.
The swipe will give you much better indications of how clean your broth is.
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shroomshine
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Re: second opinion on LC test (agar pour plate) [Re: mushboy]
#26858942 - 08/02/20 01:34 PM (3 years, 5 months ago) |
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Thank you guys for the reply!
I think that's mycelium too, but I also think that mold grew over it after a few days That's kinda like what happened on my agar plates.. mycelium starts to grow but then mold jumps from its back, takes over and eventually wins the race. I also did an (intense) smell examination of the test plate and it smells more like my grandmas closet than mushrooms  I feel like I have learned a lot in the past two weeks tho looking at agar pictures and hopefully can make out the difference better in the future!
Yes, I do regret making a pour plate because of my inexperience when it comes to interpretation. It sounded cool tho...
Well we will see. 20mL just went to grain and I have to say I find failure quite inspiring. First LC shit the bed, I don't care. I am already into months of failing (yes I grew some mushrooms, but the "perfect" run is still to come). Not going to give up! Too stoked about my new hobby and online community.
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Babnik


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Re: second opinion on LC test (agar pour plate) [Re: shroomshine]
#26859009 - 08/02/20 02:02 PM (3 years, 5 months ago) |
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If you struggle hard with agar, you can always do agar jar.
It's taken people far.
Faster than a speeding bullet, racier than a racing car.
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