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OfflineCristianosolo
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From Spores to Agar. Just a question ....
    #26857474 - 08/01/20 03:46 PM (2 days, 12 hours ago)

Recently i am trying to improve my tecnique from Spores to Agar agar. I tried with the inocultion loop, i tried witht the q~tips, i did a agar agar mix with less agar agar ....
I usually put the spores in the center of my container .
Is it possible that the mycelium is coming out not from the center but from the edge or not exacly from where i left the spores?
If the answer is yes , how is it possible?

I had few containers with new born mycelium starting from another location from where i left the spores
Thanks


Edited by Cristianosolo (08/01/20 04:51 PM)


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OfflineCapsicum Chinense
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Re: From Spores to Agar. Just a question .... [Re: Cristianosolo]
    #26857577 - 08/01/20 05:18 PM (2 days, 11 hours ago)

No, that's unlikely. Not technically impossible, but growth where you did not inoculate is probably mold.

Look up the streaking technique, there's a way to inoculate agar that streaks around the edges of the plate and can help you clean up your spores more quickly.


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OfflineTight Lunchbox
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Re: From Spores to Agar. Just a question .... [Re: Capsicum Chinense]
    #26857617 - 08/01/20 05:55 PM (2 days, 10 hours ago)

If condensation inside the dish drips down on to the media spores can be carried away to the edge of the plate when moving it around.


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Invisiblec10h12n2o
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Re: From Spores to Agar. Just a question .... [Re: Tight Lunchbox]
    #26857793 - 08/01/20 08:19 PM (2 days, 8 hours ago)

Depends how you inoculated. Probably contams or spores rolled around by too much liquid

Check the "working with spores" section of my sig agar link for a great streaking technique that will give you lots to choose from


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OfflineCristianosolo
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Re: From Spores to Agar. Just a question .... [Re: c10h12n2o]
    #26859344 - 08/02/20 05:32 PM (1 day, 11 hours ago)

Quote:

c10h12n2o said:
Depends how you inoculated. Probably contams or spores rolled around by too much liquid

Check the "working with spores" section of my sig agar link for a great streaking technique that will give you lots to choose from



Thanks ...


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InvisibleNichrome
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Re: From Spores to Agar. Just a question .... [Re: Cristianosolo]
    #26859422 - 08/02/20 06:11 PM (1 day, 10 hours ago)

Sometimes a drop of water will transport spores to the edge without you knowing.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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