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Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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OfflineEntheoGod
Entheo
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Registered: 03/06/15
Posts: 648
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Re: Agar: What Am I Doing Wrong?? [Re: wolfXsoul]
    #26856806 - 08/01/20 08:02 AM (3 years, 7 months ago)

Pressure cook them with foil around them after properly prepping the agar. Pressure cook for 30 minutes at 15 psi. Leave them to cool in the pressure cooker and remove and immediately place them inside your sab, once removed from the foil and you’ve prepped your sab, making sure to wipe each with alcohol before placing it in the prepped sab for inoculating.

That is most definitely bacteria and can either be caused by air making its way inside after they’re pressure cooked or during inoculating. I mean obviously you could have not sterilized the agar in the first place but I’m assuming you’re pressure cooking them correctly. Agar gives EVERYTHING that touches it the nutrients to grow so clean work is a MUST with it. I’m still currently working on my first agar plates and I’ve learned so much! I hope it’s not your pressure cooker just so you don’t have to fork out another 60-80$.

Good luck.🍀

Edited by EntheoGod (08/01/20 08:05 AM)

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Invisiblesandman420
Saint PP
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Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Agar: What Am I Doing Wrong?? [Re: EntheoGod]
    #26856809 - 08/01/20 08:05 AM (3 years, 7 months ago)

Double layer the micropore too.

Are the lids loose when you remove or are they still tight.

I really think if it was airborne contam from lid problem then you would have more molds in there instead of straight bacteria.


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Edited by sandman420 (08/01/20 08:06 AM)

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OfflinewolfXsoul
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Registered: 06/05/20
Posts: 12
Last seen: 3 years, 6 months
Re: Agar: What Am I Doing Wrong?? [Re: sandman420]
    #26857443 - 08/01/20 03:26 PM (3 years, 7 months ago)

Have some grain jars at about 30% right now with no issues that i can see.

I'm doing a test run at the moment with 2 different pp5 containers and 4oz glass jars to see if there's any difference.  Also, apparently I've been using the wrong amount of water for my model of instapot (1 cup vs 2 cups), so ive adjusted that.  Perhaps the 1 cup of water wasn't creating enough steam.

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