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Offlineredpillcottage
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Correct agar technique - please advise me. spore syringe to agar
    #26856496 - 08/01/20 02:25 AM (11 days, 14 hours ago)

Hey there.

Can someone point me in the right direction for the proper technique to go from spore syringe to agar?

So I vaguely remember how to isolate a pure culture way back when i did biology at uni. so you get the bacteria, fungus, or whatever sample and wipe it on the side of the agar dish, flame the loop, drag a corner across, flame the loop drag the corner. eventually, you start to get isolated cultures from one bacteria or in this case a spore. Is this something I want to do?



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Invisiblec10h12n2o
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Re: Correct agar technique - please advise me. spore syringe to agar [Re: redpillcottage]
    #26856513 - 08/01/20 02:37 AM (11 days, 14 hours ago)

Check my sig, there is a section on streaking spore syringes to agar. "Working with spores"


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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Offlinecoversall
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Re: Correct agar technique - please advise me. spore syringe to agar [Re: redpillcottage]
    #26856514 - 08/01/20 02:38 AM (11 days, 14 hours ago)



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Invisiblesandman420
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Re: Correct agar technique - please advise me. spore syringe to agar [Re: coversall]
    #26856836 - 08/01/20 10:24 AM (11 days, 6 hours ago)

you just have to squeeze out a bit after flaming the needle to get the plunger loosey goosey before trying to shoot one, else a big rush will come out...

So flame, squirt as little as possible into a sacrificial container to feel the plunger loosen up, barely press the plunger to make 1 single micro-drop on each plate of the stack without flaming anymore in between just move up the sleeve from the bottom boop boop boop.

Tilt it around a bit after wrapping the plate thats it.

Make your agar a little sofgter than normal too, like 14g instead of 20g per liter.

Do at least 10-20 plates to get a some good ones.

May as well just do a whole sleeve in one go.


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Edited by sandman420 (08/01/20 10:29 AM)


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Invisiblec10h12n2o
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Re: Correct agar technique - please advise me. spore syringe to agar [Re: sandman420]
    #26857344 - 08/01/20 04:10 PM (11 days, 52 minutes ago)

I much prefer serial streaking over just rolling the liquid around. The pic op posted is exactly what i do

It gives you waaaaay more to choose from, way more organized growth with less strains per colony in the later zones

But whatever works works


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (08/01/20 04:11 PM)


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OfflineCatsLoveHouseMusic
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Re: Correct agar technique - please advise me. spore syringe to agar [Re: c10h12n2o]
    #26857366 - 08/01/20 04:34 PM (11 days, 27 minutes ago)

I was just going to post this guys tek, the guy who posted above me. I do a mix of this guys tek^^^^ and a simple drop in the middle of agar. See what happeneds. Just be really sanitary, I go above what more probably call sanitary and my agar always works out.


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