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Offlinebrocepheushyde
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Registered: 04/16/20
Posts: 85
Last seen: 3 years, 4 months
WBS No foul odor, zero visible growth after 15 days
    #26857091 - 08/01/20 11:14 AM (3 years, 5 months ago)

Let me preface by saying that I know I need to get a pressure cooker to get bulk tubs going. Just sorta experimenting with this.

I steam sterilize in a large soup pot with a lid. I can fit like 8 quart sized jars in it comfortably but i only do 6 at a time usually.



After soaking and prepping the grains, I dried them and jarred them up. Put them in the pot raised off the bottom and fill it with the water up to 1/4 way up the jars. I then set the pot on top of 2 stove burners, both on high until the water starts boiling then reduce to medium heat so my jars dont crack. Put the lid on and leave it set for 90 minutes. Then I let it set for 24 hours, and the following day I run the same steaming process over again for 2 hours. And let them cool for a few hours before inoculation.

I made my own syringes this time around, both times. I was as sterile as I couldve been without a SAB both times, making one inside a ziplock bag (2nd one), and one inside a shot glass (the first one) with packing tape over top of it and a slit to stick my syringe thru.

I dont have a SAB yet but im working on it. I also just got my agar dishes in a couple weeks ago. Excited to get to be working with cloning and getting some actual canopies for once.

BUTTTTT I have a minor snag.

Everything looked pretty good going in both rounds. But the first 5 jars i did havent shown any signs of growth at all, so I opened the jars and sniffed them and they just smell like wet bird seed i guess is the best description. Same as it did after I prepped it both times. No foul odor, either. The first 5 were inoculated on 7/16 and havent shown any growth at all. The 6 that I inoculated on 7/18 have already shown a bit of growth. I went ahead and shook them, and thats why you dont see any mycelium in the left jar. I shook them all up fr... Ive shaken the non colonized jars a couple times to make sure that spores just arent meeting one another or something but after 2 weeks somethings gotta be up i imagine?

The jar on the left is a 7/18 jar. The jar on the right is a 7/16 jar. They look the same to me. 💁 Any guesses on whats going on here guys?

 


In some other news, I ran the same steam sterilizing process on 10 half pint popcorn jars and had a 0 contamination rate. :> I am thinking about dumping the 5x 7/16 jars out for the birds and running 5 more pcorn jars. Thoughts? They colonized pretty aggressively both in grain and in substrate. Theyve been ready for a few days but I spawned them on 7/7. Those are pictured below too juat for shits and gigs and so i dont have to clutter the board with another post. Theyre starting to pin already 2 days after swapping out lids.



Edited by brocepheushyde (08/01/20 11:17 AM)


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Offlinebrocepheushyde
Stranger
Registered: 04/16/20
Posts: 85
Last seen: 3 years, 4 months
Re: WBS No foul odor, zero visible growth after 15 days [Re: brocepheushyde]
    #26857737 - 08/01/20 07:39 PM (3 years, 5 months ago)

Any advice?


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OfflineZakkery
I'm a teapot User Gallery


Registered: 10/15/18
Posts: 293
Loc: UK Flag
Last seen: 6 days, 18 hours
Re: WBS No foul odor, zero visible growth after 15 days [Re: brocepheushyde] * 1
    #26858165 - 08/02/20 04:43 AM (3 years, 5 months ago)

By the sounds of it you likely just have dirty spawn. Spore syringe to grain is a game of chance, let alone doing it with syringes prepared using the methods you have.

Your grains might also just not be close enough to sterile after being steamed. If you're having success with the popcorn you might as well keep that up, but definitely focus most of your efforts getting set up for agar>grain. Including making a SAB. That's the recipe for long term success.

Also try to get your hands on a PC as you are already aware. The steaming works fine for agar plates and things that are a bit more resilient like PF cakes, but for grain you want that hot PC action.


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