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simonbelmont
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Registered: 07/31/20
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Last seen: 3 years, 5 months
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First Attempt
#26856196 - 07/31/20 08:27 PM (3 years, 6 months ago) |
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My first try I decided 3ccs from sponsor here in three grain bags, and pimped out a mono tub similar to the one built in North Spores how to video on YouTube. It has been nine days since inoculation and I've been stupidly configuring and reconfiguring the environment my bags are in temp from 70 to 85 now closer to 70 and no light to a room with ambient light. Seems there's no growth or signs of contams how long should I wait it out before pitching?
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,173
Loc: rural ghetto
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3ccs of what? Spores? If it is, I wouldnt get my hopes up. Spores to grain are almost 100% doomed to fail. Now, 3ccs of untested liquid culture, you might have a better chance. Study agar.
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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simonbelmont
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Registered: 07/31/20
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Last seen: 3 years, 5 months
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Yes spores okay I have looked into it a bit. I was really hoping to get away with spores and either a mono tub Tek or pf as I have no pressure cooker or flow hood petry dishes etc etc. If I did up my game to that it would be great, seems like it makes the process of spawning so much easier. Thank you for the insight. I'll keep looking into it! I know I can still use those teks with agar and with a lot more ease since you can remove contams just a matter of getting everything I need together and a clean space to do it in.
Edited by simonbelmont (07/31/20 09:50 PM)
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,173
Loc: rural ghetto
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Dont cut corners as it would mean more wasted time and materials.. if you spawn something with a bit contam to a tub, you will have wasted time and materials. But its ok. We learn as we go.
You dont need a flowhood. Build a still-air-box. You dont need petri dishes, you can use PP plastic containers or small glass jars. Get a cheap ass PC as soon as you can. Borrow some money from your older sister. Hehe
Best of luck. For germination, you can start to worry at the 2nd week mark.
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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simonbelmont
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Registered: 07/31/20
Posts: 8
Last seen: 3 years, 5 months
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Wow hey thanks a lot after further reading and your advice I will give it a shot. Nothing lost nothing gained I really appreciate the insight.
Hehe no older sister 😅 33 and have developed fascination with amateur mycology and psychedelics and their potential benefits.
Edited by simonbelmont (08/01/20 08:15 AM)
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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Quote:
pablokabute said: Spores to grain are almost 100% doomed to fail.
That's a huge exaggeration. I probably had an 85-90% success rate when I was shooting spores to grain. I only ever trusted one particularly reputable spore vendor though.
I would be more worried about the presterilized grain bags.
There are a lot of reasons I wouldn't recommend spores to grain, but "100% doomed to fail" is just false.
Make sure your grain prep is on point, and get your spores from a trusted source. It can work, but it isn't ideal.
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EntheoGod
Entheo



Registered: 03/06/15
Posts: 648
Last seen: 8 months, 14 days
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Quote:
Genghis Chron said:
Quote:
pablokabute said: Spores to grain are almost 100% doomed to fail.
That's a huge exaggeration. I probably had an 85-90% success rate when I was shooting spores to grain. I only ever trusted one particularly reputable spore vendor though.
I would be more worried about the presterilized grain bags.
There are a lot of reasons I wouldn't recommend spores to grain, but "100% doomed to fail" is just false.
Make sure your grain prep is on point, and get your spores from a trusted source. It can work, but it isn't ideal.
That’s not an exaggeration. Believe me. Spores in syringes almost 100% of the time have bacteria inside of them. I’m sure if you work with agar, then you will see this for yourself. Agar is something I’ve recently gotten into after having a literal 100% bacteria rate with every spore syringe used. Yes I got lucky with a lot of them and succeeded in putting ms syringe to grain but after moving to agar, I will NEVER put spores to grain ever again. It’s such a waste of potential and almost always performs at a lesser quality compared to substrate prepped with spawn that originated on agar.
I would say clean spawn is the first step in any of this hobby. Without it, nothing will succeed. It doesn’t matter how clean your grains are, or your substrate. An uncolonized grain is a vector for contamination. 🤷♂️ It’s best to ONLY put mushie spores in the grains instead of bacteria and cube spores. Spore syringes are full of bacteria. You just shoot them into grains and hope the myc outruns everything else.
Edited by EntheoGod (08/01/20 08:57 AM)
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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He didnt say "100% have bacteria" he said "100% doomed to fail".
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EntheoGod
Entheo



Registered: 03/06/15
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Last seen: 8 months, 14 days
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Basically same thing. Bacterial spawn CAN fruit but barely and not happily.
It nearly 100% of the time has bacteria. If you can do brf tek you can do agar.
Like NIKE says Just do it. 🤣
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The Fresh Prints
Smell ya later



Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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My point was that it can work. I'm not advocating for it. Just saying its not 100% doomed.
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,173
Loc: rural ghetto
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Yea. Spores to grain, successfully colonized, can still be spawned and yield fruits, what Im trying to imply with 100% doomed to fail is so I can scare away OP so he starts studying agar by himself. Hahahaha. But yea, slighly bacterial grain spawn can still be spawned..
But sometimes, there are unseen molds lurking inside those grains shot with spores and spawning it is 100% doomed to fail.
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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simonbelmont
Stranger

Registered: 07/31/20
Posts: 8
Last seen: 3 years, 5 months
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Okay lol gonna do pasty's agar one question....after they come out of the PC and I nocc em up how do I store them and how long till I can use them to make lc? People like you are why this forum rules. Thanks all!
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